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BIOCHEM LEC FINALS

Total questions: 99

Worksheet time: 1hrs 16mins

Name
Class
Date
1.

give 3 functions of an enzyme:

a)

protein that catalyzes chemical reactions and is catalyzed by ph, temp, and concentration

b)

speeds up reaction progress with same outcome

c)

helps regulate metabolism

d)

only found in animals

2.

pairs of reactions that occur together

a)

coupled reactions

b)

energy

c)

opposites

3.

released by absorption of the other

a)

coupled reactions

b)

energy

c)

opposites

4.

space between outer and inner membrane

a)

intermembrane space

b)

matrix

5.

enclosed by the inner membrane

a)

intermembrane space

b)

matrix

6.

is the first step - catalyzed by enzymes in the saliva, stomach, and small intestines.

a)

digestion

b)

carbohydrates

c)

protein

d)

triacylglycerols

7.

hydrolyzed into monosaccharides beginning with amylase enzymes in saliva and continuing in the small intestine

a)

digestion

b)

carbohydrates

c)

protein

d)

triacylglycerols

8.

digestion begins when stomach acid denaturesthe protein and pepsin begins to xleave the large protein backbone into smaller peptides.

a)

digestion

b)

carbohydrates

c)

protein

d)

triacylglycerols

9.

emulsified by bile secreted by the liver, then hydrolyzed by lipase into 3 fatty acids and a glycerol backbone

a)

digestion

b)

carbohydrates

c)

protein

d)

triacylglycerols

10.

monosaccharides, amino acids, and fatty acids are degraded into acetyl groups, which are then bonded to coenzyme A forming acetyl-CoA

a)

Formation of acetyl CoA

b)

Citric acid cycle

c)

Electron acid chain and oxidative phosphorylation

11.

produces energy stored as a nucleoside triphosphate and reduced coenzymes

a)

Formation of acetyl CoA

b)

Citric acid cycle

c)

Electron acid chain and oxidative phosphorylation

12.

within the mitochondria, the electron transport chain and oxidative phosphorylation produce ATP (adenosine 5'-triphosphate)

a)

Formation of acetyl CoA

b)

Citric acid cycle

c)

Electron acid chain and oxidative phosphorylation

13.

cleaves one phosphate group and provides the energy for the phosphorylation of Glucose

a)

Hydrolysis of ATP

b)

Formation of ADP and Hydrogen phosphate (HPO42-)

c)

Phosphorylation

d)

Release of energy

14.

releases 7.3kcal/mol of energy

a)

Hydrolysis of ATP

b)

Formation of ADP and Hydrogen phosphate (HPO42-)

c)

Phosphorylation

d)

Release of energy

15.

reverse reaction where ADP is added

a)

Hydrolysis of ATP

b)

Formation of ADP and Hydrogen phosphate (HPO42-)

c)

Phosphorylation

d)

Release of energy

16.

fuels physical processes when hydrolyzed to ADP

a)

Hydrolysis of ATP

b)

Formation of ADP and Hydrogen phosphate (HPO42-)

c)

Phosphorylation

d)

Release of energy

17.

fuels physical processes when hydrolyzed to ADP

a)

Hydrolysis of ATP

b)

Formation of ADP and Hydrogen phosphate (HPO42-)

c)

Phosphorylation

d)

Release of energy

18.

choose two truths for DNA fingerprinting

a)

Any types of skin cell can be obtained as a DNA fingerprint

b)

Infectious agent consisting of a DNA/RNA molecule

c)

DNA is first amplified by PCR, and then cut by restriction enzymes

d)

Produces antibodies to ward off infection

19.

Virus

a)

Any types of skin cell can be obtained as a DNA fingerprint

b)

Infectious agent consisting of a DNA/RNA molecule

c)

DNA is first amplified by PCR, and then cut by restriction enzymes

d)

Produces antibodies to ward off infection

20.

Vaccine

a)

Any types of skin cell can be obtained as a DNA fingerprint

b)

Infectious agent consisting of a DNA/RNA molecule

c)

DNA is first amplified by PCR, and then cut by restriction enzymes

d)

Produces antibodies to ward off infection

21.

Choose two truths for retrovirus

a)

Virus with an RNA core

b)

Infectious agent consisting of a DNA/RNA molecule

c)

DNA is first amplified by PCR, and then cut by restriction enzymes

d)

Synthesized by reverse transcription

22.

4 elements needed for PCR

a)

Copied segments of DNA & Two primers

b)

Glowing primers & magic wishes

c)

DNA polymerase enzyme & Nucleoside triphosphate

d)

Whispered taq and golden polymerase

23.
  • 1. Heat the DNA segment to unwind the double helix to form single strands

  • 2. Add primers that are complementary to the DNA sequence at either end of the DNA segment

  • 3. Use DNA polymerase and added nucleotides to lengthen the DNA segment

    • after each cycle, the amount of DNA is doubled, so after 20 cycles, 1m copies have been made

a)

Process DNA recombination

b)

Process of PCR

24.

THREE TYPES OF RNA

a)

tRNA

b)

rRNA

c)

mRNA

d)

pRNA

25.

One or more nucleotides is/are lost from a DNA molecule

a)

Deletion

b)

Insertion

c)

Silent

26.

sum of all chemical reactions that take place in an organism

a)

catabolism

b)

metabolism

c)

anabolism

27.

breakdown of large molecules from smaller ones; energy is generally absorbed during anabolism

a)

catabolism

b)

metabolism

c)

anabolism

28.

synthesis of large molecules from smaller ones; energy is generally absorbed during anabolism.

a)

catabolism

b)

metabolism

c)

anabolism

29.

series of consecutive reactions that can be linear/cyclic

a)

metabolic pathway

b)

linear

c)

cyclic

30.

series of reactions that generates a final product from any of the reactants

a)

metabolic pathway

b)

linear

c)

cyclic

31.

series of reactions that regenerates the first reaction

a)

metabolic pathway

b)

linear

c)

cyclic

32.

Occurs when one or more nucleotides is/are added to a DNA molecule

a)

Deletion

b)

Insertion

c)

Silent

33.

Has a negligible effect on the organism, because the resulting amino acid is identical

a)

Deletion

b)

Insertion

c)

Silent

34.

mutation passed through generations

a)

genetic diseases

b)

cystic fibrosis

c)

galactosemia

35.

extremely thick lung mucus and low pancreatic secretions

a)

genetic diseases

b)

cystic fibrosis

c)

galactosemia

36.

deficiency of enzyme needed for galactose metabolism

a)

genetic diseases

b)

cystic fibrosis

c)

galactosemia

37.

key elements for DNA recombination

a)
  • 1. DNA molecule into a new DNA segment will be inserted

  • 2. The enzyme cleaves DNA at specific locations

  • 3. Gene from the second organism will be inserted into the original DNA molecule.

b)
  1. 1. DNA requires presence of RNA

  2. 2. Recombination only occurs between homologous DNA sequences

  3. 3. Always results to a hybrid DNA molecule

c)
  1. 1. DNA requires presence of histones

  2. 2. Always occurs during DNA replication

  3. 3. Always results in creation of genetic mutation

38.

Process of DNA recombination

a)
  • 1. DNA mixes without any control or guidance.

  • 2. It then changes without using a blueprint.

  • 3. it then changes happen without any energy or effort.

b)
  • 1. Bacterial plasmid DNA is cut by the restriction endonuclease EcoRI

    • this gives a double strand of linear plasmid DNA with two ends ready to bond, called Sticky ends.

  • 2. The second sample of HUMAN DNA is cut with the same EcoRI

    • this forms human DNA segments with sticky ends -- complementary to the plasmid DNA

  • 3. Combining the two pieces of DNA (w/ DNA ligase enzyme) forms DNA containing the new segment

c)
  1. 1. Goes back to original form

  2. 2. Happens in one side

  3. 3. Changes only in nucleus

39.

give 3 rules of replication

a)

pair with T, and G must pair with C.

b)

none of the above

c)

Replication only occurs in ONE direction. Which is the template strand, from the 3' end to the 5' end

d)

New strand may either be a leading strand, or a lagging strand growing.

40.

Bases for DNA double helix

a)

Pyrimidine

b)

ribose

c)

deoxyribose

d)

Purine

41.

CHECK WHAT APPLIES FOR DNA

a)

B-form double helix. Double-stranded w/ longer nucleotides

b)

Transfer of genetic information

c)

self replicating

d)

synthesized from source

42.

CHECK WHAT APPLIES FOR RNA

a)

B-form double helix. Double-stranded w/ longer nucleotides

b)

Transfer of genetic information

c)

self replicating

d)

synthesized from source

43.

4 PARTS OF DNA

a)

ADENINE & GUANINE

b)

NONE OF THE CHOICES

c)

CYTOSINE & THYMINE

44.

CHOOSE TWO TRUTHS FOR THE CENTRAL DOGMA OF PROTEIN SYNTHESIS

a)

Information stored in DNA is used to synthesize proteins

b)

NONE OF THE CHOICES

c)

Replication uses the DNA for self-replication

45.

What is the correct order for protein synthesis

a)

Splitting of two strands > segment replication > glued together

b)

segment replication > splitting to two strands > glued together

c)

glued together > segment replication > splitting of two strands

46.

4 PARTS OF RNA

a)

ADENINE & GUANINE

b)

NONE OF THE CHOICES

c)

CYTOSINE & URACIL

47.

"IDINE" at the end

a)

Pyrimidine derivation

b)

Purine derivation

c)

Deoxyribonucleoside derivation

48.

"OSINE" at the end

a)

Pyrimidine derivation

b)

Purine derivation

c)

Deoxyribonucleoside derivation

49.

"DEOXY" at the beginning

a)

Pyrimidine derivation

b)

Purine derivation

c)

Deoxyribonucleoside derivation

50.

Effects of adding Cytidine 5'monophosphate

a)

CMP

b)

dAMP

c)

ADP

51.

Effects of adding Deoxyadenosine 5'-monophosphate

a)

CMP

b)

dAMP

c)

ADP

52.

Effects of adding Adenosine 5'-diphosphate

a)

CMP

b)

dAMP

c)

ADP

53.

Effects of adding Adenosine 5'-triphosphate

a)

ATP

b)

dAMP

c)

ADP

54.

CHOOSE 2 TRUTHS OF POLYNUCLEOTIDE

a)

Consists a backbone that is made of alternating sugar and phosphate groups

b)

Proposed by Watson and Crick in 1953

c)

Has one free phosphate group at the 5' end and one free OH group at the 3' end

d)

consists of two polynucleotide strands that wind into a right-handed double helix

55.

CHOOSE 2 TRUTHS OF DNA DOUBLE HELIX

a)

Consists a backbone that is made of alternating sugar and phosphate groups

b)

Proposed by Watson and Crick in 1953

c)

Has one free phosphate group at the 5' end and one free OH group at the 3' end

d)

consists of two polynucleotide strands that wind into a right-handed double helix

56.

Give 2 definitions of a catalyzed enzyme

a)

Catalyzes by a lower free energy

b)

2x10^3kcat(s^-1)

c)

they are permanent and indestructible

d)

changes equilibrium of action

57.

Give 2 definitions of an uncatalyzed enzyme

a)

Catalyzes by a higher free energy

b)

2x10^-8knon(s^-1)

c)

they are permanent and indestructible

d)

changes equilibrium of action

58.

Functions of citric acid cycle

a)

used by cells to generate energy from carbohydrates

b)

produces ATP directly

c)

usually is the aconitase -- binds to the subtrate citrate making isocitrate

d)

occurs in cytoplasm

59.

functions of oxido-reductases

a)

catalyze oxidation and reduction reactions in which electrons travel from one molecule to the other

b)

produces ATP directly

c)

usually is the aconitase -- binds to the subtrate citrate making isocitrate

d)

occurs in cytoplasm

60.

functions of transferase

a)

catalyze oxidation and reduction reactions in which electrons travel from one molecule to the other

b)

produces ATP directly

c)

catalyze transportation of a functional group from one molecule to another

d)

occurs in cytoplasm

61.

functions of hydrolase

a)

catalyze oxidation and reduction reactions in which electrons travel from one molecule to the other

b)

catalyze the hydrolysis of chemical bonds with the action of water (OH)

c)

catalyze transportation of a functional group from one molecule to another

d)

occurs in cytoplasm

62.

functions of lyases

a)

catalyze oxidation and reduction reactions in which electrons travel from one molecule to the other

b)

Catalyze the breakdown of various chemical bonds by means other than hydrolysis and oxidation

c)

catalyze transportation of a functional group from one molecule to another

d)

occurs in cytoplasm

63.

functions of isomerases

a)

catalyze oxidation and reduction reactions in which electrons travel from one molecule to the other

b)

Catalyze the breakdown of various chemical bonds by means other than hydrolysis and oxidation

c)

Catalyze structural shifts in molecules, causing changes in shape.

d)

occurs in cytoplasm

64.

functions of ligases

a)

catalyze oxidation and reduction reactions in which electrons travel from one molecule to the other

b)

Catalyze the breakdown of various chemical bonds by means other than hydrolysis and oxidation

c)

Catalyze structural shifts in molecules, causing changes in shape.

d)

Catalyze ligation-- the combination of pairs of substrates

65.

choose two truths for lock and key

a)

Proposed by Emil Fischer in 1894

b)

In this model, the enzyme and sometimes the substrate change shape as they interact until the active site is fully bound

c)

The enzyme and the substrate have three-dimensional shapes that fit for eachother

d)

Proposes enzyme molecules that can change their shape, depending on the interaction with the substrate.

66.

choose two truths for induced fit

a)

Proposed by Emil Fischer in 1894

b)

In this model, the enzyme and sometimes the substrate change shape as they interact until the active site is fully bound

c)

The enzyme and the substrate have three-dimensional shapes that fit for eachother

d)

Proposes enzyme molecules that can change their shape, depending on the interaction with the substrate.

67.

choose two truths for coenzymes

a)

Some enzymes require an additional chemical, called a cofactor, for catalysis to occur.

b)

In this model, the enzyme and sometimes the substrate change shape as they interact until the active site is fully bound

c)

The enzyme and the substrate have three-dimensional shapes that fit for eachother

d)

The cofactor could be a metal ion or an organic molecule, such as a vitamin. Cofactors may bind loosely or tightly to enzymes.

68.

Coenzymes are simple organic compounds that are endowed with specific structural features that allow them to help accelerate the enzymatic reactions. They function as carriers of atoms or electrons which are essential for normal metabolism.

a)

true

b)

false

69.

Nucleic acids are unbranched polymers composed of repeating monomers called NUCLEOTIDES

a)

true

b)

false

70.

A nucleotide monomer contains: monosaccharide and an n-containing base,

a)

true

b)

false

71.

the 5 types of n-containing bases are purine, pyrimidine, DNA, and RNA

a)

true

b)

false

72.

monosaccharide DNA has aldopentose/d-2-deoxyribose & RNA has aldopentose d-ribose

a)

true

b)

false

73.

Stores genetic information

a)

DNA

b)

RNA

74.

Translates genetic information

a)

DNA

b)

RNA

75.

Choose two truths for enzyme inhibition

a)

The binding of enzymes to the active sites, causes the inhibitors to reduce the compatibility of substrate and enzyme and this leads to the inhibition of Enzyme-Substrate [ES] complexes' formation. The Inhibition of forming the [ES] complex prevents the catalyzation of reactions and decreases (at times to zero) the amount of product produced by a reaction.

b)

Inhibition may be irreversible if the inhibitor attaches covalent bonds. Inhibition may be reversible if the inhibitor attaches weak bonds.

c)

none

76.

Choose two truths for competitive inhibitors

a)

chemicals that resemble an enzyme's normal substrate and compete with it for the active site.

b)

are molecules that do not enter the enzyme's active site but bind to another part of the enzyme molecule.

c)

They block the active sites from the substrate

77.

Choose two truths for non-competitive inhibitors

a)

chemicals that resemble an enzyme's normal substrate and compete with it for the active site.

b)

are molecules that do not enter the enzyme's active site but bind to another part of the enzyme molecule.

c)

The substrate is still able to access the active site, however, it alters the configuration of the enzyme so it is no longer fully functional

78.

a high temperature increases the rate of enzyme activity due to the substrate molecules moving faster making an enzyme likely to come in contact with the substrate quickly. the enzyme then becomes denatured rendering inactive.

a)

effects of temperature

b)

effects of pH

c)

effects of concentration

d)

effect of mechanical stress

79.

Enzymes have 7-8 pH and can only react to a specific pH

a)

effects of temperature

b)

effects of pH

c)

effects of concentration

d)

effect of mechanical stress

80.

choose two truths of transcription

a)

synthesis of mRNA from DNA

b)

Each triplet is called a codon

c)

U replaces T on mRNA

d)

UAC is a codon for the amino acid serine; UGC is a codon for the amino acid cysteine

81.

choose two truths of genetic code

a)

synthesis of mRNA from DNA

b)

Each triplet is called a codon

c)

U replaces T on mRNA

d)

UAC is a codon for the amino acid serine; UGC is a codon for the amino acid cysteine

82.

contains sequence of codons

a)

mRNA

b)

tRNA

c)

rRNA

83.

brings specific amino acids to peptide chain

a)

mRNA

b)

tRNA

c)

rRNA

84.

contains binding sites for protein synthesis occurrence.

a)

mRNA

b)

tRNA

c)

rRNA

85.
  • 1. begins with mRNA binding to the ribosome

  • 2. tRNA brings first amino acid ALWAYS to the codon AUG

a)

initiation

b)

elongation

c)

termination

86.

proceeds as the next tRNA molecule delivers the next amino acid, and a peptide bond forms between the two amino acids.

a)

initiation

b)

elongation

c)

termination

87.

Stops by (UAA, UAG, or UGA) then releases the complete protein

a)

initiation

b)

elongation

c)

termination

88.

Change in nucleotide sequence in a molecule of DNA

a)

mutation

b)

mutagen

c)

point mutation

89.

Possible cause of changes/mutations

a)

mutation

b)

mutagen

c)

point mutation

90.

Substitution of one nucleotide for another

a)

mutation

b)

mutagen

c)

point mutation

91.

THREE PARTS OF PYRIMIDINE

a)

CYTOSINE

b)

ADENINE

c)

THYMINE

d)

URACIL

92.

THREE PARTS OF PURINE

a)

CYTOSINE

b)

ADENINE

c)

THYMINE

d)

GUANINE

93.

rate of reaction is doubled and saturates with the substrate while affecting the rate of reaction

a)

effects of temperature

b)

effects of pH

c)

effects of concentration

d)

effect of mechanical stress

94.

What is the enzymatic rate reaction

(a)  

95.

what's the t in trna

(a)  

96.

what's the m in mrna

(a)  

97.

what's the r in rrna

(a)  

98.

Amplifies a specific portion of a DNA molecule, producing millions of exact copies

(a)  

99.

what is this

(a)