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MicroBio

Total questions: 100

Worksheet time: 1hrs 1mins

Name
Class
Date
1.

Viruses can be cultured using the same methods of bacteria and fungi

a)

True

b)

False

2.

Pasture developed a vaccine for what virus ?

a)

Chicken pox

b)

Rabies

c)

measles

d)

TLS

3.

Viruses were discovered by filtering infectious fluid through a filter designed to trap what ?

a)

Bacteria

b)

Algae

c)

Fungi

d)

Membranes within the cell

4.

What word is Latin for Poison ?

a)

Disease

b)

Rabies

c)

Bacteria

d)

Virus

5.

Why were infectious fluids designed to pass through porcelain filters?

a)

to calm the virus

b)

To stimulate the bacterial cell

c)

To trap bacteria

d)

to release bacteria

6.

What did the experiment of the bacteria through the porcelain filters teach us ?

a)

That bacteria was the cause of disease

b)

That disease was caused by something smaller than bacteria

c)

That viruses couldn't fit through the filters

d)

That Viruses are larger than Bacteria

7.

What types of cells can Viruses infect

a)

Only bacterial cells

b)

Only animal cells

c)

Only prokaryotic ones

d)

all of them

8.

How do viruses reproduce

a)

They take over the living cells machinery

b)

They slide in the cell on their own

c)

They can reproduce themselves

d)

They put themselves in our bloodstream first

9.

Are viruses alive ?

a)

yes

b)

no

10.

How large are viruses ?

a)

100-150 nm

b)

300-600nm

c)

20-200 nm

d)

10-100nm

11.

Viruses are not organisms, instead what are they ?

a)

pathogens

b)

diseases

c)

bacteria

d)

Infectious Particles

12.

protein shells are also known as

a)

Capsid

b)

Nuclei

c)

Cell Envelope

d)

Chemical Machinery

13.

Can cells make their own proteins ?

a)

yes

b)

no

14.

How many enzymes do Viruses have ?

a)

200+

b)

none

c)

1-5 depending on the type

d)

only 1

15.

What percentage of the bacterial chromosomes is composed of viral genes ?

a)

20-25%

b)

5-10%

c)

10-20%

d)

30-60%

16.

why are some viruses considered "mimi" viruses ?

a)

because they are mimicking a microbe

b)

because they are larger than they average

c)

They are considered extra small

d)

They are very long and fat

17.

Which of these is a cocci occurring in pairs?

a)

Streptococci

b)

Diplococci

c)

Streptococci

d)

Staphylococci

18.

Based on the diagram above, the flagella arrangement of bacteria cell is known as ___________.

a)

Petritrichous

b)

Monotrichous

c)

Lophotrichous

d)

Amphitrichous

19.

To differentiate between Gram positive and Gram negative bacteria (a)   procedure will be used.

20.

A Gram negative cell wall is (a)   than a Gram positive one.

21.

Which of the following statement is TRUE regarding Gram positive bacteria?

a)

Cell wall have high lipid content

b)

Lipopolysacharides layer is present

c)

crystal violet stain is retained

d)

Have 2 layers of cytoplasmic membrane.

22.

What is the function of Pilus in prokaryotic?

a)

for swimming movement

b)

Attachment to surface

c)

Transfer of DNA to other cells

d)

Serve as defense mechanism

23.

Lipopolysaccharides also can be known as

a)

Exotoxin

b)

Endotoxin

24.

(a)   is ability to produce disease in host organism.

25.

Endotoxin is extremely potent than exotoxin.

a)

True

b)

False

26.

Bacteria endopsore usually occur in Gram positive bacteria.

a)

True

b)

False

27.

A slippery outer covering in some bacteria that protects them from phagocytosis by host cells is

a)

cell wall

b)

capsule

c)

peptidoglycan

d)

Teichoic acid

28.

Pleomorphic is the term used particularly for bacteria having

a)

One shape

b)

Rod

c)

Spiral

d)

Variable shape

29.

The space between the cytoplasmic membrane and the outer membrane is called the periplasm.

a)

True

b)

False

30.

Bacteria are best measured in nanometers?

a)

True

b)

False

31.

Prokaryotic and eukaryotic cells have many differences, but they also share some common features. Which of the following may be found in either type of cell?

a)

Golgi bodies

b)

mitochondria

c)

ribosomes

d)

nucleus

32.

Father of Microbiology is

a)

Louis Pasteur

b)

Lister

c)

Robert Koch

d)

Leeuwenhoek

33.

The main feature of prokaryotic organism is

a)

Absence of locomotion

b)

Absence of nuclear envelope

c)

Absence of nuclear material

d)

Absence of protein synthesis

34.

Hanging drop method for mobility study was given by

a)

Koch

b)

Pasteur

c)

Hooke

d)

Lister

35.

E.coli was first isolated by

a)

Hooke

b)

Pasteur

c)

Koch

d)

Escherich

36.

The bacteria were first observed in the year 1675 by

a)

Carolus Linnaeus

b)

Alexander Fleming

c)

Anton Von Leenuwenhoek

d)

Robert Brown

37.

The study of Fungi is

a)

Algalogy

b)

Mycology

c)

Bacteriology

d)

Phycology

38.

Microorganisms which responsible for producing during rainy season is

a)

Bacillus sp.

b)

Actinomycetes sp.

c)

Saccharomyces sp.

d)

None of the above

39.

Aeromicrobiology refers to

a)

Study of living microbes

b)

study of Algae

c)

Study of living microbes which are suspended in the air

d)

none of the above

40.

The bacterium that is most commonly used in genetic engineering is

a)

E.coli

b)

Klebsiella

c)

Bacillus substilus

d)

Staphylococcus auerus

41.

A book of Micrographia was written by

a)

Leeuwenhoek

b)

Robert Hooke

c)

Fracastaro

d)

Pastuer

42.

Counter stain used in Gram's Staining is

a)

Crystal Violet

b)

Ethanol

c)

Safranin

d)

Malachite green

43.

Gram positive bacteria

a)

E.coli

b)

Lactobacillus sp.

c)

Pseudomonas sp.

d)

All of the these

44.

Gram staining is an example of

a)

Acid fast staining

b)

Simple staining

c)

Negative staining

d)

Differential staining

45.

Scanning Electron Microscopy (SEM) is best used to study

a)

small internal structures

b)

surface morphology

c)

Living, unstained cells

d)

all of these

46.

Transmission Electron Microscopy (TEM) is best used to study

a)

small internal structures

b)

surface morphology

c)

crystalline structure

d)

all of these

47.

Which of the following is not a prokaryote?

a)

Bacteria

b)

Paramecium

c)

Cyanobacteria

d)

Rickettsia

48.

A 40X objective and 10X produce a magnification of

a)

50X

b)

100X

c)

400X

d)

4000X

49.

The first physician to make practical application of the germ theory of disease to surgery was

a)

Joseph Lister

b)

Louis Pastuer

c)

Robert Koch

d)

Edward Jenner

50.

The primary use of Koch postulates is to

a)

Clearly identify and characterize a particular microorganism

b)

Isolate microbes from diseased animals

c)

demonstrate that disease is caused by microorganisms

d)

develop vaccines for specific disease

51.

Mordant used in Gram's Staining is

a)

Crystal Violet

b)

Safranin

c)

Ethanol

d)

Iodine

52.

Lipid contents are more in

a)

Gram negative bacteria

b)

Gram positive bacteria

c)

Both

d)

None of the above

53.

The ability of microscope to distinguish between two objects into two separate objects is called

a)

Resolving power

b)

Numerical aperture

c)

Wavelegth

d)

Distance

54.

Light gathering capacity of a microscope is

a)

Angular aperture

b)

Numerical aperture

c)

Wavelength

d)

All of the above

55.

In electron microscope, what material is used as an objective lens?

a)

Magnetic coils

b)

Superfine glass

c)

Electrons

d)

Aluminium foils

56.

Which one of the following produces images?

a)

Light Microscope

b)

Transmission Electron Microscope

c)

Scanning Electron Microscope

d)

All of the above

57.

Mycology is the study of __________.

a)

fungi

b)

bacteria

c)

algae

d)

protozoa

58.

Pasteur developed vaccine against _____________.

a)

small pox

b)

cow pox

c)

anthrax

d)

measles

59.

Phycology is the study of _____________.

a)

bacteria

b)

viruses

c)

fungi

d)

algae

60.

_______________ coined the term "cell"?

a)

Joseph Lister

b)

Edward Jenner

c)

Louis Pasteur

d)

Robert Hooke

61.

Hooke's microscope developed in 1670s was named ____________

a)

Circa

b)

Leica

c)

Micrographia

d)

Zeiss

62.

The experiment above was conducted by ________________ to disprove spontaneous generation.

a)

Pasteur

b)

Spallanzani

c)

Needham

d)

Redi

63.

_________________ demonstrated that hand washing can prevent the spread of disease.

a)

Jenner

b)

Lister

c)

Semmelweis

d)

Koch

64.

____________ was named Father of chemotherapy after his discovery of salvarsan.

a)

Jenner

b)

Ehrlich

c)

Semmelweis

d)

Lister

65.

______________________ is a process in mining that extracts valuable metals from a low-grade ore with the help of microorganisms.

a)

Bioleaching

b)

Biomineralization

c)

Biotransformation

d)

Biosorption

66.

______________ is the ability of biological materials including microorganisms to accumulate heavy metals from wastewater through metabolically mediated or physico-chemical pathways of uptake.

a)

Biosorption

b)

Bioleaching

c)

Biomineralization

d)

Bioaccumulation

67.

Identify the image of microbiologist

a)

Robert Brown

b)

Pasteur

c)

Kotch

d)

Hesse

68.
A microorganism that can cause disease is called a(n) ________________.
a)
pathogen
b)
host
c)
virulence
d)
autogenous
69.
Forms of life that are most resistant to extremes of heat and dryness are __________.  
a)
bacteria
b)
viruses
c)
fungi
d)
spores
70.

I am a unicellular prokaryote with a peptidoglycan cell wall. What am I?

a)

Virus

b)

Bacteria

c)

Archaea

d)

Protist

71.

Known as the Father of Microbiology due to his work with "animacules" found on his own teeth is:

a)

Jonas Salk

b)

Robert Koch

c)

Semmelweiss

d)

Anton Van Leeuwenhoek

72.
Which best compares bacteria and viruses?
a)
Bacteria are smaller than viruses.
b)
Bacteria cause disease, but viruses do not.
c)
Bacteria are living organisms, but viruses are not.
d)
Bacteria have genetic material, but viruses do not.
73.

who is father of microbiology ?

a)

Louis pasture

b)

Alexender

c)

jenner

d)

Ronaldo

74.
Which was most likely classified as a pandemic?
a)
People in eleven states were sickened after eating beef contaminated with the E. Coli bacteria. The tainted beef was traced to a meat-packing plant.
b)
Two camps, in close proximity to each other, experienced an outbreak of cholera. The infection was traced to contaminated drinking water.
c)
A measles outbreak in Indiana was attributed to children whose parents did not have them vaccinated for measles.
d)
During 1918 to 1919, an estimated 20-40% of the world's population suffered from the Spanish flu.
75.
The bacteria that causes Bubonic Plague is transmitted to humans by fleas. Which term best describes the role of the fleas in transmitting Bubonic Plague?
a)
Decomposer
b)
Pathogen
c)
Parasite
d)
Vector
76.
Which best explains how a virus affects other organisms?
a)
Viruses increase the survival of other organisms.
b)
Viruses immediately destroy all cells they come in contact with.
c)
Viruses live and reproduce in between the cells of other organisms.
d)
Viruses invade the cells of other organisms, using them to reproduce.
77.

Identify a gram positive bacteria.

a)

E. coli

b)

Salmonella typhi

c)

Staphylococcus aureus

d)

Pseudomonas putida

78.

Ames test is used to identify------

a)

A bacteria

b)

A mutagen

c)

A virus

d)

A disease

79.

Who is considered as the father of Microbiology.

(a)  

80.

The main feature of prokaryotic organism is

a)

Absence of locomotion

b)

Absence of nuclear envelope

c)

Absence of nuclear material

d)

Absence of protein synthesis

81.

Hanging drop method for mobility study was given by

a)

Koch

b)

Pasteur

c)

Hooke

d)

Lister

82.

The bacterium that is most commonly used in genetic engineering is

a)

E.coli

b)

Klebsiella

c)

Bacillus substilus

d)

Staphylococcus auerus

83.

Identify the fungus.

a)

Aspergillus sp.

b)

Penicillium sp.

c)

Rhizopus sp.

d)

Mucor sp.

84.

Dermal infections caused by group of fungi is called:

a)

Dermatomycosis

b)

Influenza

c)

Measles

d)

Mumps

85.

An inflammation of stomach and bowels due to eating or drinking microbial contaminated meal is:

a)

Dermatomycosis

b)

Food poisoning

c)

Foodborne infection

d)

Nosocomial infection

86.

The illness caused by bacterial or other toxins in food is:

a)

Dermatomycosis

b)

Food poisoning

c)

Foodborne infection

d)

Nosocomial infection

87.

Drinkable water is called:

a)

Hard water

b)

Non-potable water

c)

Potable water

d)

Soft water

88.

Microorganisms that can harm us are called:

a)

Normal flora

b)

Non-Pathogenic

c)

Pathogens

d)

Probiotics

89.

Microorganisms benefits us by providing:

a)

Antibiotics

b)

Pathogenes

c)

Spoilage of crops

d)

Spoilage of food

90.
What is the first thing you should do?
a)

put on gloves

b)

wash your hands

c)

sterilize your workspace

d)

put on gown

91.

What is the goal of aseptic techniques

a)

To prevent contamination from the worker only

b)

To prevent contamination from the environment only

c)

To prevent contamination from the worker and the environment

92.
If you see someone in a laboratory using aseptic technique, what might that person be doing?
a)
Talking to a co-worker
b)
Looking through a microscope
c)
Properly cleaning equipment
d)
Adjusting the room temperature
93.

What is the bunsen burner used for?

a)

Nutrients for bacteria

b)

Sterilizing equipment

c)

Collecting/spreading bacteria

d)

Stopping contamination

94.

Avoid contamination of materials during microbiological activity

a)

Safety shower

b)

Fume chamber

c)

Laminar flow cabinet

d)

Fire extinguisher

95.

What is the instrument that used for sterilizing media and apparatus?

a)
b)
c)
d)
96.

What do you do if you spill a microbial culture?

a)

Tell the instructor

b)

Clean it up before anyone notices

c)

Lick it up

d)

Wipe it up with your shirt

97.
 If you do not understand a direction or part of a lab procedure, you should 
a)
skip it and go on to the next part.
b)
ask the instructor before proceeding
c)
try several methods until something works
d)
figure it out as you do the lab
98.

What type of shoes should you wear to lab?

a)

flip flops

b)

socks and sandals

c)

closed toed shoes

d)

no shoes required

99.
What is the last thing you should do?
a)

take off your gloves

b)

wash your hands

c)

sterilize your workspace

d)

put on gown

100.

One word For Quiz organizor?Type Honestly❤?

4 lines

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