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WorksheetsMIDTERM LAB EXAM HEMATOLOGY
Total questions: 61
Worksheet time: 53mins
What does the hematocrit test measure?
Total plasma volume
Volume of platelets
Volume of red blood cells compared to total blood volume
Volume of white blood cells
What is the ideal use of the hematocrit test?
To measure blood pressure
To follow the progress of anemic or bleeding patients
To diagnose infections
To assess liver function
Which equipment is used in the microhematocrit procedure?
Blood pressure cuff
Capillary tube
Syringe
Test tube
How long should the blood sample be centrifuged in the microhematocrit test?
2 minutes
10 minutes
4 minutes
6 minutes
What is the first step in the microhematocrit determination process?
Sealing the capillary tube
Centrifuging the sample
Performing a capillary puncture
Filling the capillary tube
Interpret hematocrit result.
Female: 48%
Increased
Decreased
Normal
Interpret hematocrit result.
Male: 35%
Increased
Decreased
Normal
Interpret the result based on initial visualization of microhematocrit tube.
Increased
Decreased
Normal
Identify LABEL 1
(a)
Identify LABEL 3
(a)
What does ESR stand for?
Erythrocyte Sediment Rate
Erythrocyte Serum Ratio
Erythrocyte Sedimentation Rate
Erythrocyte Serum Rate
What is the primary purpose of the ESR test?
To diagnose specific diseases
To indicate inflammation or tissue injury
To measure blood pressure
To assess kidney function
What is the normal range of ESR for adult males using the Wintrobe method?
0-30 mm/hr
<50 mm/hr
0-20 mm/hr
>50 mm/hr
Which factor does NOT affect the sedimentation rate in the ESR test?
Temperature of the environment
Patient's age
Concentration of alpha and beta globulin
Rouleaux formation
What is the first stage of the ESR process?
Settling or sedimentation
Reading the results
Rouleaux formation
Packing of the sedimentation cell column
What type of blood is used for the ESR test?
Citrated blood
Whole blood
Oxalated blood
Heparinized blood
How long should the tube be allowed to stand for the ESR test?
15 minutes
2 hours
1 hour
30 minutes
What is the expected ESR range for children?
0-9 mm/hr
0-20 mm/hr
0-30 mm/hr
0-13 mm/hr
What is the significance of the sedimentation rate results?
They measure blood viscosity
They indicate the presence of inflammation
They assess liver function
They are used to diagnose specific diseases
Which of the following is NOT a material used in the ESR test?
Pasteur pipette
Wintrobe tube
Syringe
Stop watch
What is the primary use of a hemocytometer?
To analyze blood chemistry
To measure blood pressure
To determine blood type
To count blood cells
Which of the following is NOT a reason to use a hemocytometer?
Automated cell counters are unavailable
Blood cell counts are extremely low
To measure blood glucose levels
To count cells in body fluids
What is the depth of the scales on a Neubauer slide?
0.05 mm
0.1 mm
0.2 mm
0.3 mm
What color bead is found on an RBC pipette?
Red
White
Blue
Green
What is the dilution factor when using a Thoma pipette for RBC counting?
1:400
1:200
1:100
1:300
What is the area of the smallest square on the grid of a hemocytometer?
0.001 mm²
0.0025 mm²
0.005 mm²
0.01 mm²
Which solution is NOT mentioned as a diluting fluid for RBC counting?
Isotonic saline
Hayam’s solution
Gower’s solution
Sodium bicarbonate solution
What is the normal range of erythrocytes for males?
6.0 – 7.0 x 10^6/μL
5.5 – 6.5 x 10^6/μL
4.2 – 5.4 x 10^6/μL
3.6 – 5.0 x 10^6/μL
What should be done to the Neubauer chamber before use?
Heat to sterilize
Clean with 70% ethanol
Soak in water
Rinse with saline
Which pipette has a larger bulb?
Neither has a bulb
Both are the same
WBC pipette
RBC pipette
What is the volume correction factor if the counted volume is 0.02 cumm?
100
25
200
50
What is the main reason for falsely low RBC counts?
Collection from hemoconcentrated area
Blood dilution with tissue fluid
Errors in calculation
Improper pipetting
What is the purpose of using an anti-coagulant in the diluting fluid?
To increase viscosity
To preserve RBC shape
To prevent cell clumping
To enhance color
Which objective lens is used first to focus on the large central square?
40x
10x
100x
4x
What is being counted in the five red small squares?
(a)
What is being read in the 4 blue large squares?
(a)
Count the RBC in the Upper left Corner. (save your answer here in scratch paper to be used in another questions)
(a)
Count the RBC in the Lower left corner (save your answer here in scratch paper to be used in another questions)
(a)
Count the RBC in the middle square (E) (save your answer here in scratch paper to be used in another questions)
(a)
Count the RBC in the Upper Right corner (save your answer here in scratch paper to be used in another questions)
(a)
Count the RBC in the lower right corner. (save your answer here in scratch paper to be used in another questions)
(a)
Compute the RBC count you counted in the 5 squares in the last 5 questions.
(a)
Interpret the calculated RBC count from the previous question. FEMALE
Decreased
Increased
Normal
What is the purpose of a buffy coat smear?
For WBCs < 1.0×10^9/L
For blood typing
For RBCs
For platelets
Which type of blood smear is prepared from fresh blood?
Thick smear
Spun smear
Buffy coat smear
Wedge smear
What can excessive anticoagulant in a specimen cause?
Change in RBC morphology
Increase in WBC count
Decrease in platelet count
Thickening of blood
What is the main component of a blood smear?
Anticoagulant
Glass slide
Blood film
WBCs
Which type of blood cell is classified as a granulocyte?
Monocyte
Neutrophil
Platelet
Lymphocyte
What is the effect of old blood on RBC morphology?
Increases oxygen capacity
Causes spiculated cells
Makes them larger
No effect
What is the recommended time frame for preparing a wedge blood smear?
4 to 5 hours
2 to 3 hours
Immediately
1 hour
What type of blood smear is used for detecting blood parasites?
Spun smear
Buffy coat smear
Thick blood smear
Wedge smear
What is the role of a clean glass slide in blood smear preparation?
To reduce drying time
To enhance color
To increase thickness
To prevent contamination
What is the primary purpose of a peripheral blood smear?
To check for heart disease
To perform a blood transfusion
To analyze blood cell morphology
To measure blood pressure
Which of the following is NOT a common clinical indication for peripheral blood film analysis?
Suspected chronic myeloproliferative disease
High blood sugar levels
Unexplained jaundice
Unexplained cytopenia
What is the size range of a normal red blood cell?
10-12 μm
8-10 μm
6-8 μm
4-6 μm
Which staining method is commonly used for blood smears?
Hematoxylin and Eosin Stain
Romanowsky Stains
Fluorescent Stain
Silver Stain
What is the lifespan of platelets?
20-30 days
4-9 days
10-15 days
1-3 days
Which type of white blood cell is characterized by 2-5 nuclear lobes?
Basophil
Monocyte
Eosinophil
Neutrophil
What is the normal value range for neutrophils in a leukocyte differential count?
75-90%
50-70%
25-40%
0-10%
What is the method used for automated blood smear analysis?
Manual Counting
Erlich Method
Wright Stain
DiffMaster
Which of the following is a method of blood smear preparation?
Cross-sectional method
Longitudinal method
Battlement method
All of the above
