WorksheetsENTERIC BACTERIA PART 1
Total questions: 28
Worksheet time: 18mins
All Enterobacteriaceae are Cytochrome oxidase negative, except which genus?
(a)
This genus of enteric organisms are generally non-motile at 37C, but motile at room temperature.
(a)
Scientist who first described E. coli ( complete name)
(a)
Causative agent of “MONTEZUMA’S REVENGE” (acronym only)
(a)
The binding unit of this toxin binds with GM1 ganglioside receptor
Verotoxin 1
ETEC LT toxin
EHEC ST toxin
Binds to glomerular endothelium and inactivates von Willebrand factor-cleaving protease
Verotoxin 1
ETEC LT toxin
EHEC ST toxin
Pathologic condition characterized by microangiopathic hemolysis, severe thrombocytopenia and Kidney Failure.
(answer in full)
(a)
Results from the transformation of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP).
lysis of phagocytic vacuole and bacterial replication into the cell cytoplasm
cessation of protein synthesis
hypersecretion of fluids and electrolytes into the lumen of the intestines
Binding site of Verotoxin 1 B subunit
GM1 ganglioside receptor
Globotriaosylceramide
Translocated intimin receptor
Causes loss of intestinal barrier function through disruption of tight junctions and induces host cell death through apoptosis.
Translocated intimin receptor
intimin
Escherichia coli secreted protein F
O157 : H7
ETEC
EIEC
EPEC
EHEC
EAEC
On which media could we initially identify EHEC?
MAC
EMB
HEA
BAP
Mucus secretion and formation of a thick biofilm is characteristic of which E. coli strain?
(use acronym)
(a)
Which organism/s may also produce the green metallic sheen produced by E. coli on EMB?
Klebsiella
Citrobacter
Enterobacter
Salmonella
Non-lactose fermenting Klebsiella
K. rhinoscleromatis
K. oyxtoca
K. ozaenae
K. pneumoniae subsp. pnuemoniae
Indole (+) Klebsiella spp (use this format: E. coli)
(a)
Positive result for Moeller KCN Broth
dissolution/ clear solution
turbidity
blackening
formation of red color
Antibiotic therapy disrupts the normal gut microbiota and promotes the overgrowth of Klebsiella oxytoca with subsequent production of cytotoxin called (a) .
Antibiotic-associated hemorrhagic colitis resolves spontaneously within 2-3 days when the offending antibiotics are discontinued.
TRUE
FALSE
Causative agent of donovanosis (use format: E. coli
(a)
Fish-eye colonies are produced by which group (genus) of enteric organisms?
(a)
Caused a nationwide (U.S) outbreak of septicemia resulting from contaminated IV fluids
Enterobacter cloacae
Enterobacter agglomerans
Enterobacter aerogenes
Rarely isolated from blood cultures.
Enterobacter cancerogenus
Enterobacter hormaechei
Enterobacter gergoviae
Pink to red pigment of Serratia
(a)
To which of the following assays does Salmonella demonstrate a (+) result?
KCN growth
Lysine decarboxylation
ONPG
Only urease (+) Providencia species
Providencia alcalifaciens
Providencia heimbachae
Providencia rettgeri
Providencia stuartii
When different Proteus species swarm towards each other, a line of inhibited growth results where strains meet. This is known as (a)
Most notable characteristic of Proteus colonies
burnt chocolate odor
swarming
hemolysis
