NEW
Font size
S
M
L
XL
WorksheetsPglo
Total questions: 13
Worksheet time: 8mins
Name
Class
Date
1.
What is the circular piece of DNA found in bacteria called?
a)
homologous chromosome
b)
sister chromatid
c)
plasmid
d)
arabinose
2.
Making changes in the DNA of an organism is
a)
selective breeding
b)
artificial selection
c)
genetic engineering
d)
genetic transformation
3.
Which gene WAS NOT included in the +pGlo plasmid?
a)
antibiotic resistance gene
b)
green fluorescent protein gene
c)
hemophilia gene
4.
What abbreviation represents the sugar molecule needed to "turn on" the green fluorescent protein gene?
a)
LB
b)
AMP
c)
ARA
d)
+pGlo
5.
What is the name of the plasmid we used for lab?
a)
GFP
b)
ampR
c)
araC
d)
pGLO
6.
Which gene is responsible for making the bacteria resistant to ampicillin?
a)
ampR
b)
pGLO
c)
araC
d)
GFP
7.
What was used to cut the plasmid to enable us to insert the desired gene?
a)
ligase
b)
restriction enzymes
c)
GFP
d)
pGLO
8.
What did we use to create temperature differential to sweep plasmid inside
a)
heat shock
b)
ice
c)
arabinose
d)
CaCl2
9.
Which plate will have bacterial growth and bacterial GLOWth?
a)
LB
b)
LB/Amp
c)
LB/Amp/Ara
d)
All
10.
What is used to ensure the bacteria transformed with the gene for antiobiotic resistance as only the transformed bacteria will grow on this medium?
a)
ampicillan
b)
arabinose
c)
LB
d)
GFP
11.
Which gene is responsible for the bacteria glowing?
a)
ampR
b)
araC
c)
GFP
d)
pGLO
12.
Which gene is responsible for "turning on" the glow?
a)
ampR
b)
ARAc
c)
GFP
d)
LB
13.
Which protein is responsible for allowing the bacteria to grow in the presence of ampicillin?
a)
GFP
b)
araC
c)
Beta Lactamase
d)
Restriction Enzyme
Reset
