WorksheetsCrispr Ihub
Total questions: 16
Worksheet time: 13mins
What does the acronym CRISPR stand for?
Controlled Reservative Image Spectacle Palindromic Recounts
Clustered Regularly-Interspaced Short Palindromic Repeats
CRISPR can edit DNA. What organisms naturally has CRISPR?
Viruses
Humans
Plants
Bacteria
Why do bacteria have CRISPR? Check all that apply.
To be able to remember which viruses they have been infected with
To be able to edit virus DNA
To cut apart virus DNA thereby stopping the virus infection
To be able to edit their own DNA
A PAM is
a 15-20 bp sequence snipped from a viral genome that serves as a tracer DNA to locate viruses later
a 3 bp sequence that adjacent to viral DNA in a CRISPR array
a girl's name popular in the 1960s
where the DNA is cut
What is the role of the guide RNA?
It helps the cell repair the DNA
ensures that the Cas9 enzyme cuts at the right point in the genome
Recognizes PAM sequences and opens up the DNA
makes a cut across both strands of the DNA
How does the guide RNA help the cell know where to cut the DNA?
Guide RNA helps to find the PAM
Guide RNA is complementary to the DNA sequence
Guide RNA always binds to the same sequence of DNA
Like restriction sites, they are always palindromes
The following sequence is a known GENE MUTATION that geneticists would like to remove. What would be the guide RNA they need to build into a CRISPR-Cas9 enzyme?
TTA CCG
AAU GGC
AAT GGC
TTA CCG
GCC ATT
What does the cas9 protein do?
Prevents viruses from entering bacteria
Cut RNA
Cut DNA
Binds to RNA
How does CRISPR edit DNA? Check ALL that apply.
CRISPR chemically changes A's to C's
CRISPR cuts the DNA and the cell makes errors when it tries to fix the broken DNA
CRISPR cuts the DNA and the cell can use a similar DNA sequence as a template to try to fix the DNA
CRISPR changes all PAM's so that they are no longer able to be recognized
CRISPR-Cas9 is a type of ________ system found in bacteria.
digestive
endocrine
circulatory
immune
CRISPR-Cas9 was discovered in which of the following organisms?
protists
fungi
bacteria
viruses
Scientists have adapted it to turn it into a biotechnology tool for _______ DNA.
building
editing
destroying
synthesizing
The enzyme portion of this complex is called ________.
CRISPR
Replicase
Lysozyme
Cas9
What type of RNA is used as a GPS of sorts, to find or locate specific genes?
mRNA
gRNA
tRNA
rRNA
What is PAM?
a 3 letter sequence for binding on the target DNA
the main gene portion of the DNA
the cut site for Cas9
a 10 letter sequence for binding on the target DNA
Once the DNA and RNA are bound, Cas9 is activated to cut the DNA ____ upstream from the binding sequence.
1 base
3 bases
6 bases
10 bases
