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WorksheetsMEDICAL MICROBIOLOGY
Total questions: 10
Worksheet time: 5mins
Sandwich ELISA plate coated with
Ag
Ab
Ag-Ab complex
Protein
Enzyme converts a colorless substrate to a colored product, indicating the presence of
Ag
Ag : Ab binding
Ab
Hormone
ELISA assay is a widely used to detect
Hormones and antibodies
Bacteria
Viruses
All of the above
.... ELISA
Direct
Sandwich
Indirect
.... ELISA
Direct
Sandwich
Indirect
.... ELISA
Direct
Sandwich
Indirect
The first layer on the ELISA plate will be the
Antigen
antibody
virus
substrate
Why is it necessary to wash the samples repeatedly?
it is important to keep the lab wells clean
it is important to wash away all bound and unbound antibodies from the wells
it is important to wash away the unbound antibodies
washing the wells is just solid lab practice
What is a primary antibody?
the second antibody used to detect foreign particle
second cell produced by the macrophage
particle produced by the antigen
the first antibody used to detect foreign particle
When a positive + result occurs ELISA, then one could conclude that...
a + result always means illness
it is a false + and no illnes
the antibody is present
