WorksheetsIMMUNOLOGY
Total questions: 10
Worksheet time: 5mins
What is the role of Properdin in complement pathway regulation?
Cleave and inactivate C3b and C4b
Accelerate the dissociation of C3 convertase
Stabilize the alternative pathway C3 convertase
Inhibit MAC formation
What is a Membrane Attack Complex?
A Thing Made Out Of C9
A Thing Made Out Of C6, C7, C8 And C9
An Attack System
Impossible To Know
Which pathway of complement activation involves the interaction between lipid-carbohydrate complexes on the surface of microbes and complement protein factors B, D, and P?
Classical pathway
Alternative pathway
Lectin pathway
Terminal pathway
What is the main function of complement related to the bursting of microbial cells?
Immune complex clearance
Cytolysis
Phagocytosis
Inflammation
Why do you assay your samples in duplicates?
To act as a control of the assay
To test for pipetting error
Reproducibility of results
All of the above
The first layer on the ELISA plate will be the
Antigen
antibody
virus
substrate
Why is it necessary to wash the samples repeatedly?
it is important to keep the lab wells clean
it is important to wash away all bound and unbound antibodies from the wells
it is important to wash away the unbound antibodies
washing the wells is just solid lab practice
Why is a positive and negative control necessary in the setup of ELISA?
positive controls are equal to negative controls
ELISA is subject to errors; if controls fail the results are untrustworthy
ELISA is a well run test that typically does not need controls
negative and positive controls are needed to exclude all results
What is a primary antibody?
the second antibody used to detect foreign particle
second cell produced by the macrophage
particle produced by the antigen
the first antibody used to detect foreign particle
When a positive + result occurs ELISA, then one could conclude that...
a + result always means illness
it is a false + and no illnes
the antibody is present
