WorksheetsGlycolysis Quiz
Total questions: 43
Worksheet time: 22mins
Glycolysis
does not require O2 to generate energy.
requires O2 to generate energy.
is inhibited by O2.
rate is increased in the presence of O2
The fate of pyruvate produced during glycolysis depends primarily on the availability of
NAD+ to keep the pathway going.
molecular oxygen.
ADP for conversion to ATP.
coenzyme A for further metabolism of pyruvate.
phosphoric acid for the synthesis of ATP.
In aerobic metabolism, what is the fate of pyruvate produced by glycolysis?
Pyruvate loses carbon dioxide, and the remaining two carbon atoms become linked to coenzyme A.
Pyruvate loses carbon dioxide, producing acetaldehyde, which, in turn, is reduced to ethanol.
Pyruvate is reduced to lactate.
None of these
In humans, pyruvate can be converted to
acetyl-CoA only.
lactate only.
ethanol only.
acetyl-CoA and lactate.
Which of the following is not an end product of glucose metabolism via either aerobic or anaerobic means?
ethanol
carbon dioxide
lactate
fructose
all of these are end products of glucose metabolism
What is the net ATP yield per glucose during glycolysis?
1
2
3
4
6
In the conversion of glucose to pyruvate, how many of the actual steps involve electron transfer?
none
1
2
3
4
The order of compounds in the conversion of glucose to pyruvic acid is as follows: (PEP =phosphoenolpyruvate)
Fructose-bisphosphate, fructose-6-phosphate, 1,3-phosphoglyceric acid, 3-phosphoglyceric acid, PEP.
Fructose-6-phosphate, fructose-bisphosphate, PEP, 1,3-phosphoglyceric acid, 3-phosphoglyceric acid.
Fructose-6-phosphate, fructose-bisphosphate, 1,3-phosphoglyceric acid, 3-phosphoglyceric acid, PEP.
Fructose-6-phosphate, fructose-bisphosphate, 3-phosphoglyceric acid, 1,3-phosphoglyceric acid, PEP.
T/F: Biofuels are related to glycolysis because fermentation is an end process of anaerobic glycolysis
True
False
T/F: Many carbohydrate sources can produce ethanol.
True
False
Which of the following terms describes an enzyme that transfers a phosphate group from ATP to a substrate?
kinase
isomerase
mutase
dehydrogenase
T/F: The DG values for glycolytic reactions at physiological conditions may be exergonic, even though the DG°' at "standard" conditions, may be endergonic.
True
False
The reactions where glucose is converted to glucose 6-phosphate and fructose 6-phosphate is converted to fructose 1,5-bisphosphate are examples of:
exergonic reactions
priming reactions
phosphorylation reactions
kinase reactions
all of these
The phosphorylation of glucose to glucose 6-phosphate:
is so strongly exergonic that it does not require a catalyst.
is an exergonic reaction not coupled to any other reaction.
is an endergonic reaction that takes place because it is coupled to the exergonic hydrolysis of ATP.
is an exergonic reaction that is coupled to the endergonic hydrolysis of ATP.
The enzyme glucokinase:
phosphorylates a number of different sugars, including glucose, fructose, and mannose.
specifically phosphorylates glucose rather than other sugars.
is the only kinase involved in glycolysis.
none of the above.
The binding of glucose to hexokinase
is an example of lock-and-key binding of a substrate to the active site of an enzyme.
is an example of induced-fit binding of a substrate to the active site of an enzyme.
differs from the binding of substrates to other kinases.
is not well characterized.
The phosphorylation of fructose 6-phosphate to fructose-1,6-bisphosphate is the committed step in glycolysis because:
it is the rate-limiting step.
it is the most strongly exergonic step in the pathway.
fructose 1,6-bisphosphate can undergo no other reactions than those of glycolysis.
two phosphate groups are involved.
Which of the following sugars can be a substrate for hexokinase?
glucose
fructose
mannose
all of these
none of these
Which of the following enzymes interconverts an aldose and a ketose?
kinase
isomerase
mutase
dehydrogenase
phosphorylase
Which enzyme is the key regulatory enzyme in glycolysis?
Glyceraldehyde-3-phosphate dehydrogenase
Enolase
Phosphofructokinase
Aldolase
Which of the following exercise(s) allosteric control in the reaction of phosphofructokinase?
ATP
fructose 2,6-bisphosphate
both of these
neither of these
The reaction of fructose 1,6-bisphosphate to give glyceraldehyde-3-phosphate and dihydroxyacetone phosphate is an example of:
a reverse aldol condensation.
hydrolysis.
oxidation.
dehydration.
The equilibrium for isomerization of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate is favored because:
the standard free energy is negative
glyceraldehyde-3-phosphate is being continuously drained off for the subsequent reaction in the glycolytic pathway
the value of the equilibrium constant favors the reaction
it is driven by the hydrolysis of ATP
The isomerization of dihydroxyacetone phosphate to give glyceraldehyde 3-phosphate:
is catalyzed by the enzyme triose phosphate isomerase.
requires several enzymes.
requires coenzyme A.
requires thiamine pyrophosphate.
The step that commits the cell to metabolize glucose is catalyzed by:
hexokinase.
phosphoglucomutase.
aldolase.
phosphofructokinase.
The equilibrium for the formation of glyceraldehyde-3-phosphate from dihydroxyacetone phosphate is driven by:
the negative free energy change for the reaction.
having the product of the reaction continuously consumed.
coupling to ATP hydrolysis.
none of these
Which of the following enzymes catalyzes the cleavage of fructose bisphosphate into two 3-carbon units?
Aldolase
Enolase
An isomerase
A mutase
None of these enzymes carries out that reaction.
T/F: Every reaction in a metabolic pathway must have a negative DG, or the pathway cannot run.
True
False
T/F: The reaction with the largest negative ÄG is the hexokinase reaction
True
False
T/F: The overall pathway of glycolysis has a negative ÄG
True
False
Which of the following terms describes an enzyme that catalyzes electron transfer reactions?
dehydrogenase
isomerase
kinase
phosphatase
Which of the following enzymes forms a thioester using a cysteine residue as a key intermediate?
hexokinase
triose phosphate isomerase
glyceraldehyde-3-phosphate dehydrogenase
enolase
In glycolysis, ATP is synthesized by:
substrate-level phosphorylation.
oxidative phosphorylation.
photophosphorylation.
both substrate-level and oxidative phosphorylation.
all three of the above methods.
Enolase catalyzes
the cleavage of fructose bisphosphate into two 3-carbon units.
the dehydration of 2-phosphoglycerate.
the conversion of phosphoenolpyruvate to pyruvate.
the conversion of glucose-6-phosphate to fructose-6-phosphate.
T/F: The nicotinamide-binding region in glyceraldehyde-3-phosphate dehydrogenase is similar to all other NAD binding domains.
True
False
T/F: During glycolysis, ATP is synthesized from ADP and a phosphate group transferred from an acid anhydride
True
False
Which of the following is required for substrate-level phosphorylation?
The substrate must contain multiple phosphate groups.
Molecular oxygen must present.
The standard free energy of the hydrolysis reaction is more negative than that for hydrolysis of the new phosphate compound being formed.
All of the above are necessary for substrate-level phosphorylation.
T/F: The amino acid cysteine is important in adding a second phosphate to glyceraldehyde phosphate in the glyceraldehyde-3-phosphate dehydrogenase reaction.
True
False
Which of the following enzymes of glycolysis is not involved in regulation of the pathway?
Hexokinase
Phosphofructokinase
Aldolase
Pyruvate kinase
All of these proteins regulate glycolysis.
Which of the following glycolytic enzymes forms a mixed anhydride from phosphoric acid?
hexokinase.
phosphofructokinase.
glyceraldehyde-3-phosphate dehydrogenase.
phosphoglycerate kinase.
pyruvate kinase.
How many enzymes of glycolysis are control points for the pathway?
1
2
3
4
All the enzymes serve as control points.
Which of the following steps in glycolysis directly produces ATP?
Glyceraldehyde-3-phosphate dehydrogenase reaction
Hexokinase reaction
Phosphoglycerate kinase reaction
Phosphofructokinase reaction
Which molecule acts as an allosteric inhibitor of phosphofructokinase in glycolysis?
ATP
ADP
Glucose-6-phosphate
Fructose-1,6-bisphosphate
