WorksheetsELISA and Immunology Quiz
Total questions: 25
Worksheet time: 6hrs 15mins
What does ELISA stand for?
Enhanced Ligand Immuno Specific Assay
Enzyme Linked Immunosorbent Assay
Enzyme Linked Inhibitory Serological Assay
Endocrine Linked Immuno Specific Assay
What is the primary function of antibodies?
To produce hormones
To identify and neutralize foreign objects
To carry oxygen
To digest food
What type of protein are antibodies?
Alpha globulin
Delta globulin
Gamma globulin
Beta globulin
What is an antigen?
A type of hormone
A substance that stimulates antibody production
An enzyme that digests proteins
A protein that transports oxygen
What does an immunoassay detect?
Blood glucose only
Antigen or antibody in a sample
Enzymes only
Hormones only
Which of the following is NOT a type of ELISA?
Competitive ELISA
Sandwich ELISA
Indirect ELISA
Direct Induction ELISA
In competitive ELISA, what happens when more sample antigen is present?
More labeled antigen is retained
Stronger signal
Less labeled antigen is retained
No change in signal
What is coated on the plate in Sandwich ELISA?
Antigen
Enzyme
Buffer
Antibody
In indirect ELISA, what is added to detect the primary antibody?
Blocking buffer
Secondary antibody
Antigen
Labeled antigen
What does the enzyme in ELISA do?
Bind directly to antigen
Change substrate into a colored product
Produce antibodies
Inhibit antigen binding
Monoclonal antibodies are produced by:
Polyclonal mixture
Hybridomas
Serum plasma
Bacteria
What is the function of blocking buffer in ELISA?
Promote antigen binding
Prevent non-specific binding
Activate enzyme
Enhance color change
Which ELISA type involves enzyme-linked primary antibodies?
Indirect ELISA
Sandwich ELISA
Competitive ELISA
All of the above
What is the first step in an ELISA experiment?
Add enzyme
Read absorbance
Label the 96-well plate
Draw standard curve
What is used to interpret ELISA results quantitatively?
Histogram
Pie chart
Standard curve
Line graph
What is measured in an ELISA assay?
Weight
Volume
Absorbance
Fluorescence
Which axis represents concentration in a standard ELISA curve?
X-axis
Y-axis
Z-axis
None
Which axis represents absorbance in ELISA graph?
X-axis
Y-axis
Z-axis
None
Why is ELISA used in pregnancy tests?
To detect glucose
To detect HCG hormone
To measure hemoglobin
To detect insulin
What does a strong color change in indirect ELISA indicate?
High antigen concentration
Low antibody concentration
High primary antibody concentration
High enzyme activity
What is the analyte in an immunoassay?
Only antibody
Only antigen
Antibody or Antigen
Neither
Polyclonal antibodies are found in:
Antiserum
Monoclonal culture
ELISA plate
White blood cells
Which organism is commonly used to produce antibodies in large quantity?
Mouse
Rabbit
Goat
Fish
What binds to the antigen in indirect ELISA first?
Substrate
Secondary antibody
Primary antibody
Enzyme
How is the unknown concentration in ELISA determined?
From enzyme activity
From signal strength
From standard curve
By dilution
