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secretttt q2

Total questions: 93

Worksheet time: 58mins

Name
Class
Date
1.

What tests are used to determine and products of

glucose fermentation?

a)

A. Methyl red and Voges-Proskauer

b)

B. SIM and LDC

c)

C. Urease test and Simmons citrate

d)

D. None of the above.

2.

Lactose is a disaccharide consisting of _____ and _____ connected by a galactoside bond.

a)

A. Sucrose and fructose

b)

B. Glucose and Frustose

c)

C. Glucose and Galactose

d)

D. Galactose and Sucrose

3.

______ is structurally similar to lactose, but is more readily transported through the bacterial plasma membrane and does not require β-galactoside permease.

a)

A. Sucrose

b)

B. SIM

c)

C. Urease

d)

D. ONPG

4.

Polynucleotide composed of repeating purine and

pyrimidine mononucleotide monomeric units.

a)

A. Protein

b)

B. Lipid

c)

C. Carbohydrate

d)

D. DNA

5.

Oxidase positive organism except:

a)

A. Pseudomonas

b)

B. Moraxella

c)

C. Serratia

d)

D. Campylobacter

6.

Indicator used in Simmon Citrate Utilization:

a)

A. Bromthymol purple

b)

B. Bromthymol blue

c)

C. Methyl red

d)

D. Phenol red

7.

Rapid urease positive:

a)

A. Salmonella

b)

B. Pantoea

c)

C. Klebsiella

d)

D. Providencia

8.

The following are results for Pseudomonas aeruginosa except:

a)

A. Cetrimide (-)

b)

B. MR (-)

c)

C. Citrate (+)

d)

D. Nitrate reduction (+)

9.

The _____ is a numeric value that expresses how close the organism is to the mean profile of an organism suspected to be a match.

a)

A. Similarity index

b)

B. SD

c)

C. Coefficient of Variance

d)

D. Similarity profile

10.

The production of H2S is indicated by a ____, and a ___ color after the addition of Kovac’s reagent is positive for indole.

a)

A. Black precipitation, pink to red

b)

B. White precipitation, red

c)

C. Gas production, purple

d)

D. None

11.

A semi-solid agar medium used to detect motility and indole and ornithine decarboxylase production.

a)

A. MIO

b)

B. PAD

c)

C. SIM

d)

D. TSI

12.

Most useful in conjunction with TSI in screening stool specimens for the presence of enteric pathogens differentiating salmonella spp.

a)

A. MIO

b)

B. PAD TSI

c)

C. LIA

d)

D. SIM

13.

Decarboxylation occurs ______

a)

A. Anaerobically only

b)

B. Aerobically

c)

C. Both A and B only

d)

D. None

14.

Determines whether an organism possesses the enzyme that deaminates phenylalanine to phenylpyruvic acid

a)

A. PAD

b)

B. TSI

c)

C. SIM

d)

D. LIA

15.

The enzyme that hydrolyzes lactose into glucose and galactose.

a)

A. β-galactosidase

b)

B. LCAT

c)

C. Lipase

d)

D. β-galactoside permease

16.

Clouding of the medium or spreading growth from the line of inoculation.

a)

A. Fermentation

b)

B. Motility

c)

C. Urease hydrolysis

d)

D. Oxidation

17.

Prokaryotes and Eukaryotes are chemically similar in the:

a)

A. They possess DNA & RNA

b)

B. Food metabolism

c)

C. Reproduce sexually

d)

D. Both possess organelles

18.

These are heterotrophic eukaryotes that obtain nutrients through absorption.

a)

A. Algae

b)

B. Viruses

c)

C. Bacteria

d)

D. Fungi

19.

They are the smallest infectious particles (virions). They are neither prokaryotic/eukaryotic.

a)

A. Algae

b)

B. Viruses

c)

C. Bacteria

d)

D. Protozoa

20.

Prokaryotes are characterized by the following except:

a)

A. DNA is enclosed by nuclear membrane

b)

B. Do not possess membrane bound organelles

c)

C. DNA is not associated by histone

d)

D. Cell wall is made of peptidoglycan

e)

E. None of these

21.

The peptidoglycan layer of bacteria is composed of:

a)

A. N-acetylmuramic acid & N-acetylgalatosyl transferase

b)

B. N-acetylglucosamine and N-acetyl-d-glucosyl transferase

c)

C. Galactosyl transferase and N-acetylgalactosyl transferase

d)

D. N-acetyl-d-glucosamine and N-acetyl-d-muramic acid

22.

Gram negative bacteria possess a cell wall which is composed of:

a)

A. Peptidoglycan and phospholipid

b)

B. Phospholipid, Lipopolysaccharide, and peptidoglycan

c)

C. Polysaccharide and peptidoglycan

d)

D. Peptidoglycan and lipoprotein

23.

Gram positive bacteria possess a cell wall which is composed of:

a)

A. Peptidoglycan and phospholipid

b)

B. Phospholipid, Lipopolysaccharide, and peptidoglycan

c)

C. Polysaccharide and peptidoglycan

d)

D. Peptidoglycan and lipoprotein

24.

Plasma membrane is made up of phospholipid bilayer which contains:

a)

A. Protein group (Pollar) and fatty acids (nonpollar)

b)

B. Carbohydrate molecules (pollar) and fatty acids (non pollar)

c)

C. Fatty acid (non polar) and phosphate group + glycerol

d)

D. Carbohydrate molecules (polar) and Protein

25.

All bacteria are non or not acid fast except:

a)

A. Mycobacterium

b)

B. Mycoplasma

c)

C. Ureaplasma

d)

D. Acinetobacter

26.

All are non-acid-fast stains except,

a)

A. India ink

b)

B. Auramine

c)

C. Acridine Orange

d)

D. Calcoflour White

27.

Most pathogenic bacteria grow best at:

a)

A. Neutral pH

b)

B. Modern temperatures

c)

C. high temperature

d)

D. A & B

28.

Bacteria replicate by:

a)

A. Mitosis

b)

B. Meiosis

c)

C. Binary fission

d)

D. Both A & B

29.

Organisms that can survive in the presence of oxygen but do not use oxygen on metabolism

a)

A. Aerobes

b)

B. Obligate Aerobes

c)

C. Anaerobes

d)

D. Aerotolerant Anaerobes

30.

Bacteria contain extra information on small circular pieces of extrachromosomal:

a)

A. Pili

b)

B. Fimbriae

c)

C. Plasmids

d)

D. Endospore

31.

It is the uptake and incorporation of naked DNA into

a bacterial cell.

a)

A. Tranduction

b)

B. Transformation

c)

C. Mutation

d)

D. Conjugation

32.

It is the transfer of genetic material from a donor bacterial strain to a recipient strain.

a)

A. Transduction

b)

B. Transformation

c)

C. Mutation

d)

D. Conjugation

33.

It is the transfer of bacterial genes by bacteriophage from one cell to another.

a)

A. Transduction

b)

B. Transformation

c)

C. Mutation

d)

D. Conjugation

34.

It is an anaerobic process carried out by both obligate and facultative anaerobes

a)

A. Fermentation

b)

B. Respiration

c)

C. Both

d)

D. None of these

35.

It is an efficient energy generating process in which molecular oxygen is the final electron acceptor

a)

A. Fermentation

b)

B. Respiration

c)

C. either

d)

D. None of these

36.

Chocolate agar is an example of which of the following?

a)

A. Non selective media

b)

B. Selective media

c)

C. Differential media

d)

D. Enriched media

37.

Which of the ff is an example of a selective and differential medium?

a)

A. Blood agar

b)

B. Chocolate agar

c)

C. MacConkey Agar

d)

D. Modified Thayer Martin

38.

What is the primary stain used in fluorescent staining?

a)

A. Carbol fuchsin

b)

B. Rhodamine auramine

c)

C. Kinyoun's method

d)

D. Ziehl Neelsen method

39.

It is known as the Hot Method in acid fast staining:

a)

A. Kinyoun’s method

b)

B. Ziehl neelsen method

c)

C. Pappenheim’s

d)

D. Baumgarten

40.

What is the best staining method used to detect

Mycobacterium organisms in clinical samples?

a)

A. Acid fast staining

b)

B. Gram Staining

41.

The slightly acid fast organism

a)

A. Nocardia

b)

B. Mycobacterium

c)

C. Mycoplasma Neisseria

42.

It provides nutritional growth requirements to support highly fastidious organisms such as Haemophilus spp. And Neisseria gonorrhoeae:

a)

A. BAP

b)

B. MAC

c)

C. CHOC

d)

D. SSA

43.

What media is best used to characterize gram negative rods if it is LF or NLF?

a)

A. MAC

b)

B. TCBS

c)

C. Skirrow agar

d)

D. MSA

44.

It is a reaction caused by enzymatic or toxin activity of bacteria

a)

A. Streptolysin

b)

B. Erythrogenic toxin

c)

C. Streptokinase

d)

D. Hemolysis

45.

A comparative examination of the morphology of organisms growing on various media

a)

A. Plate reading

b)

B. Gram Staining

c)

C. Biochemical test

d)

D. Culture media

46.

It is a partial lysing of erythrocytes on BAP.

a)

A. Alpha hemolysis

b)

B. Hyaluronidase

c)

C. Beta hemolysis

d)

D. Alpha prime

47.

What is the organism that produce alpha hemolysis on BAP.

a)

A. C. Perfringens

b)

B. Neisseria

c)

C. Streptococcus pneumonia

d)

D. S. agalactiae

48.

It is the complete clearing of erythrocytes on BAP.

a)

A. Double hemolysis

b)

B. Alpha prime

c)

C. Beta hemolysis

d)

D. Alpha hemolysis

49.

This medium does not display true hemolysis.

a)

A. SSA

b)

B. BAP

c)

C. CHOC

d)

D. HEA

50.

It describe as large medium, small or pinpoint growth in a solid medium:.

a)

A. Growth

b)

B. Colony

c)

C. Culture

d)

D. Media

51.

Pili antigens are associated with:

a)

A. S. aureus

b)

B. S. pyogenes

c)

C. S. pneumoniae

d)

D. N. gonorrhea

e)

E. None of these

52.

Process of forming protein from specific RNA molecule:

a)

A. Replication

b)

B. Translation

c)

C. Transcription

d)

D. Mutation

53.

Process of forming RNA from specific DNA molecule:

a)

A. Replication

b)

B. Translation

c)

C. Transcription

d)

D. Mutation

54.

Salmonella typhi as well as other organism a which are surrounded by flagella are said to be

a)

A. Atrichous

b)

B. Lopotrichus

c)

C. Amphitrichious

d)

D. Peritrichious

55.

Simple chlorophyllous organisms, unicellular, others are aggregation of similar cell with little or no differentiation in complex structure or function.

a)

A. Bacteria

b)

B. Fungi

c)

C. Algae

d)

D. Protozoa

e)

E. Virus

56.

Smallest infectious particle, obligate intracellular

parasite possessing one type of nucleic acid

a)

A. bacteria

b)

B. Algae

c)

C. virus

d)

D. fungi

e)

E. protozoa

57.

Sodium desoxychollate and other bile salt will inhibits:

a)

A.Gram positive

b)

B.Gram negative

c)

C Both

d)

D None

58.

Sodium azide may be added to culture media to inhibits:

a)

A.Gram positive

b)

B gram negative

c)

C. Both

d)

D none

59.

Special media used to isolate Vibrio species.

a)

A. Bordet-Gengou Agar

b)

B. Buffered Charcoal Yeast Extract

c)

C. Cystine-Glucose Agar

d)

D. Thiosulfate Citrate Bile-Salts

60.

Staphylococcus aureus taken from a pure culture

smeared as Gram negative cocci. What part of the

staining procedure must be wrong?

a)

A. Over decolorization

b)

B. Underdecolorization

c)

C Understaining

d)

D. Thick bacterial smear

61.

Sterilization procedure used on labile substances

such as serum, plasma, vitamins and

carbohydrates.

a)

A Moist heat

b)

B. Dry heat

c)

C. filtration

d)

D. all

e)

E. none of these

62.

Studies on biodegradation of toxic waste:

a)

A. Immunology

b)

B. Microbial ecology

c)

C. Microbial Genetic

d)

D. Microbial Physiology

e)

E. Molecular Biology

63.

Substance added to a culture media to prevent the swarming of Proteus except

a)

A. Penicilin

b)

B. NaCl

c)

C. Increase agar

d)

D. Chloral hydrate

64.

Substance responsible for Acid-fastness:

a)

A. hydroxy methoxy acid

b)

B. Phospholipid

c)

C. Peptidoglycan

d)

D. Butyric acid

e)

E. teichoic acid

65.

Thayer Martin medium is a culture medium to

Isolate:

a)

A Clostridium

b)

B Serratia

c)

C Neisseria

d)

D Campylobacter

66.

The acidic dye used in Gram Staining:

a)

A. carbol fuchsin

b)

B. methylene blue

c)

C safranin

d)

D. Crystal violet

e)

E. Iodine

67.

The action of heat sterilization to bacteria cells:

a)

A. alteration of microbial content

b)

B. Dissociation of CHO and Protein

c)

C. Coagulation and Denaturation of protein

d)

D. Spores inactivation

e)

E. Inhibition of protein synthesis

68.

The average size of bacteria

a)

A 0.05 - 2.0 um.

b)

B. 0.005 - 2.0 um.

c)

C. 0.2-5.0 um

d)

D. 2.0-5.0 um

e)

E. 0.04-2.0 um

69.

The basic dye used in gram staining:

a)

A. Carbol fuchsin

b)

B. Methylene blue

c)

C. Safranin

d)

D. Crystal Violet

e)

E. Iodine

70.

The basic dye used in gram staining:

a)

A. Carbol fuchsin

b)

B. Methylene blue

c)

C. Safranin

d)

D. Crystal Violet

e)

E. Iodine

71.

The causative agent of tuberculosis:

a)

A. Leptospira interrogans

b)

B. Listeria monocytogenes

c)

C. Lactobacillus acidophillus

d)

D. Legionella pneumophila

e)

E. none of these

72.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

72 Autoclave
A. 100 deg.C

B. moist heat

(a)  

73.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

73 B.anthracis

A. spore (+)
B. aerobic

(a)  

74.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

74. Blood agar
A. chocolate
B. hemin

(a)  

75.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

75. C. diphtheriae
A. granulated
B. LAMB

(a)  

76.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

76. Catalase Test
A. Lactase
B. Fermentation

(a)  

77.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

77. Citrate Utilization
A. NH3
B. Bromthymol blue

(a)  

78.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

78. Coagulase Test
A. Citrated plasma
B. Gel Formation

(a)  

79.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

79. Differential Stain
A. India Ink
B. Nigrosin

(a)  

80.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

80. DNase Test
A. Clearing
B. Inhibition

(a)  

81.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

81. EMB
A. Lactose Fermenter
B. Yellow

(a)  

82.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

82. Gram Positive
A. Binian
B. Stem-Ster

(a)  

83.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

83. Gram Positive
A. Cell wall
B. Peptidoglycan

(a)  

84.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

84. Granule
A. Polysaccharide
B. Virulence

(a)  

85.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

85. Hemolysis Test
A. Lysis of RBC
B. Clearing

(a)  

86.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

86. Hydrolysis of Starch
A. Iodine solution
B. Clearing

(a)  

87.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

87. Indol Production
A. Ehrlich reagent
B. Ether

(a)  

88.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

88. Indol Production
A. Ehrlich's reagent
B. 40% KOH

(a)  

89.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

89. LIA
A. decarboxylation
B. Red

(a)  

90.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

90. M. tuberculosis
A. Acid Fast
B. Mycolic Acid

(a)  

91.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

91. Microscope
A. Hooke
B. Leeuwenhoek

(a)  

92.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

92. MR Test
A. Dextorse
B. Acidity

(a)  

93.

A. if A correct

B. if B correct

C. if both A & B are correct in one principle
D. If Both A & B are correct but in different principle
E. both A and B are incorrect

93. MSA
A. Sucrose
B. Phenol Red

(a)  

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