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MICR210 - Progress Test 2 (LO4-6)

Total questions: 39

Worksheet time: 20mins

Name
Class
Date
1.

What bacteria is this test used to identify?

a)

Campylobacter jejuni

b)

Vibrio cholerae

c)

Helicobacter pylori

d)

Aeromonas hydrophila

2.

A key symptom associate with this disease is 'rice water stool'.

a)

Vibrio cholerae

b)

Yersinia enterocolitica

c)

Plesiomonas shigelloides

d)

Shigella sonnei

3.

What can you determine from this triple sugar iron slant?

a)

The organism produced H2S gas

b)

The organism fermented glucose

c)

The organism fermented sucrose or lactose

d)

The organism produced gas

4.

A patient hooked their finger with a fish hook. Swelling and discharge appeared the next day and the wound was cultured.

BA: grey NH

Gram: Gram-negative bacilli

Oxidase: positive

What can you presume is the identification of this organism?

a)

Campylobacter jejuni

b)

Plesiomonas shigelloides

c)

Vibrio cholerae

d)

Enterotoxigenic E.coli

5.

A technologist reported Escherichia coli based on the following test results.
Gram: gnb

Oxidase: negative

TSI: A/A gas

Motility = pos

Indole = pos

Lysine = pos

Urea = pos

Citrate = neg

What test needs to be repeated for this ID to be valid?

a)

Indole

b)

Lysine

c)

Urea

d)

Citrate

6.

What culture media from a stool culture is incubated at 42°C?

a)

CIN

b)

STEC

c)

CAMP

d)

XLD

7.

Which of the following colonial morphologies is suggestive of Proteus mirabilis?

a)

b)

c)

d)

8.

Identify the bacteria based on the biochemical reactions:

a)

Escherichia coli

b)

Proteus mirabilis

c)

Klebsiella pneumoniae

d)

Citrobacter freundii

9.

What member of Enterobactericeae would have this biochemical reaction?

a)

Citrobacter freundii

b)

Klebsiella oxytoca

c)

Yersinia enterocolitica

d)

Morganella morganii

10.

Which of the following organisms will be motile at 37°C?

a)

Klebsiella pneumoniae

b)

Shigella sonnei

c)

Yersinia enterocolitica

d)

Enterobacter freundii

11.

What would the O-F Glucose test result be for an organism with this TSI result?

a)

b)

c)

12.

Serratia marcescens is described as pigmented, making it impossible to determine lactose fermentation. What biochemical test can be used to determine its ability to ferment lactose?

a)

O:F test

b)

ONPG

c)

VP

d)

Indole

13.

Interpret this nitrate reductase test.

Note; Sulfanilic acid, a-napthylamine and zinc dust were added to the tube)

a)

Nitrate negative

b)

Nitrate positive

c)

Nitrite positive

d)

Invalid

14.

A shiny grey BH bacteria is growing on the BA and has a grape-like odor. What is the expected oxidase result?

a)

positive

b)

negative

15.

Interpret this OF test result for Stenotrophomonas maltophlia.

a)

Oxidizer

b)

Non-Utilizer

c)

Fermenter

d)

Invalid

16.

What is the treatment of choice for Stenotrophomonas maltophilia?

a)

Penicillin

b)

Metrodidazole

c)

Nitrofurantoin

d)

Sulfamethoxazole trimethoprim

17.

Which Gram stain most closely resemble Acinetobacter baumannii?

a)

b)

c)

18.

A 5-year-old female is bit by her cat and presents to emergency with swelling and pus in the right index finger. What is the most likely pathogen for this source?

a)

Proteus mirabilis

b)

Pasteurella multocida

c)

Achromobacter xylosoxidans

d)

Haemophilus influenzae

19.

What test is used to differentiate between Haemophilus influenzae and Haemophilus parainfluenzae?

a)

BA with staph streak

b)

Oxidase

c)

Porphyrin

d)

Cefinase

20.

What is the identity of this organism that was isolated from a 3-month old baby that was unvaccinated?

a)

Neisseria gonorrhoeae

b)

Haemophilus influenzae

c)

Streptococcus agalactiae

d)

Moraxella catarrhalis

21.

An anaerobic bacteria growing on the VITK fluoresces chartreuse. What is the most likely identification of this organism?

a)

Prevotella sp.

b)

Porphyromonas sp.

c)

Bacteroides fragilis group

d)

Fusobacterium sp.

22.

Name the organism.

BA: no growth

CHOC: sm grey

Gram: Gram negative diplococci

Oxidase: positive

Carbohydrate Utilization: glucose pos; maltose neg; lactose neg; sucrose neg

a)

Neisseria meningitidis

b)

Neisseria lactamica

c)

Neisseria gonorrhoeae

d)

Moraxella catarrhalis

23.

Interpret the following aerotolerance test:
CHOC: sm grey

VITK: sm grey

Gram: Gram positive cocci

a)

Strict aerobe

b)

Strict anaerobe

c)

Facultative anaerobe

d)

Capnophile

24.

A Gram-negative cocci organism is Butyrate esterase positive. What is the likely identification.

a)

Haemophilus influenzae

b)

Neisseria gonorrhoeae

c)

Moraxella catarrhalis

d)

Pasteurella multocida

25.

Interpret the MIL

a)

Motility: pos

Indole: pos

Lysine: neg

b)

Motility: neg

Indole: neg

Lysine: neg

c)

Motility: pos

Indole: neg

Lysine: pos

d)

Motility: neg

Indole: neg

Lysine: pos

26.

A smelly anaerobe is isolate on a VITK with the following results. What is the identification of the organism?

Gram: Gram positive bacilli, large

Reverse CAMP: negative

Nagler: negative

Toxin production: positive

a)

Campylobacter jejuni

b)

Clostridiodes difficile

c)

Clostridium perfringens

d)

Fusobacterium nucleatum

27.

An organism growing on VITK after 10 days shows white colonies that resemble a molar tooth. Sulfur granules were noted in the aspirate that was submitted.

What is the Gram stain of this organism?

a)

Gram-positive bacilli, branching

b)

Gram-positive bacilli, large

c)

Gram-negative coccobacilli

d)

Gram-negative diplococci

28.

Name the organism that was isolated from a urine sample:

BA: grey glistening NH, mucoid

MAC: LF mucoid

Oxidase: negative

TSI: A/A gas

Motility: nonmotile

Indole: positive

Lysine: positive

VP: positive

a)

Escherichia coli

b)

Klebsiella oxytoca

c)

Enterobacter cloacae

d)

Citrobacter freundii

29.

What organism would the DNase test be helpful at identifying?

a)

Staphylococcus saprophyticus

b)

Moraxella catarrhalis

c)

Pasteurella multocida

d)

Veillonella sp.

30.

What bacteria are empirically treated with penicillin? Select all that apply.

a)

Streptococcus pyogenes

b)

Pasteurella multocida

c)

Bacteroides fragilis

d)

Pseudomonas aeruginosa

31.

What stool pathogen is nonmotile (Genus only)?

(a)  

32.

KVC disks are performed on Fusobacterium necrophorum. What are the expected results?

a)

K= resistant

V= resistant

C= variable

b)

K= resistant

V= resistant

C= resistant

c)

K= sensitive

V= resistant

C= sensitive

d)

K= resistant

V= sensitive

C= resistant

33.

What is the presumptive organism ID?

BA: grey NH, lavender-green hue

Gram: gnb

Oxidase: negative

OF: Oxidizer

Nitrate: variable

Motility: motile

MAC: NLF

a)

Pseudomonas aeruginosa

b)

Acinetobacter baumanii

c)

Stenotrophomonas maltophilia

d)

Achromobacter xylosoxidans

34.

Which of the following is NOT true about Enterobacteriaceae?

a)

All are oxidase negative

b)

All are described as grey glistening NH

c)

All are Gram-negative bacilli

d)

All can ferment glucose

35.

A mannitol fermenter is seen on the Cefsulodin-Irgasan-Novobiocin agar. What biochemical test would help differentiate the stool pathogen from NF?

a)

Motility

b)

Citrate

c)

Rhamnose

d)

Oxidase

36.

Shigella species can be identified by isolating its specific somatic antigens found on the cell wall. What type of antigens are these commonly known as?

a)

S-antigens

b)

H-antigens

c)

K- antigens

d)

O-antigens

37.

A strain of Pseudomonas aeruginosa is identified as a Carbapenemase-Producing Organism (CPO). What antibiotic will be ineffective for treatment?

a)

Sulfamethoxazole-trimethoprim

b)

Ticarcillin

c)

Meropenem

d)

Tobramycin

38.

What biochemical test used to identify Enterobacteriaceae detects neutral-end products from glucose fermentation?

a)

Voges-Proskauer (VP)

b)

Citrate

c)

Indole

d)

Triple Sugar Iron (TSI)

39.

What biochemical test can help differentiate Peptostreptococcus anaerobius from other anaerobic Gram-positive cocci?

a)

KVC disks

b)

SPS disk

c)

reverse CAMP

d)

Indole