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Biology End of Semester Review 2025

Total questions: 69

Worksheet time: 2hrs 53mins

Name
Class
Date
1.

Cells pass through a G2 checkpoint before entering mitosis. Ideally, if DNA damage is detected, the cells do not enter mitosis until the damage is repaired. Why is DNA damage repaired before cells enter mitosis?

a)

So that another round of DNA synthesis does not have to take place

b)

So that the cytoplasm can be divided equally between the two daughter cells

c)

So that healthy daughter cells are produced, allowing the organism to continue growing

2.

What process results in two complete strands of DNA from one original strand?

a)

DNA Transcription

b)

DNA Replication

c)

RNA Translation

3.

Two DNA molecules are the result of DNA replication. Which of the following is a true statement regarding DNA replication?

a)

Each new DNA molecule is made up of one new strand and one original strand.

b)

Each DNA molecule has two original strands.

c)

One of the DNA molecules has two new strands while the other has two original strands.

4.

Scientists observe that in certain turtle species, eggs incubated in cooler parts of the nest will hatch a greater proportion of male offspring, while eggs incubated in warmer parts of the nest will hatch a greater proportion of female offspring.

Which statement about cell differentiation best explains the scientists’ observations?

a)

The sex genes that produce male offspring become denatured when the eggs are incubated at high temperatures.

b)

incubation temperatures during embryonic development affect the expression of sex genes.

c)

Environmental temperatures during mating determine the sex of the offspring.

5.

As unspecialized cells mature, what controls their development into specific cell types?

a)

The rate at which the cells grow

b)

The genes expressed within the cells

c)

The nutrients available to the cells

6.

Which of the following factors can influence gene expression?

a)

a) Genetic variation

b)

b) Environmental factors

c)

c) Both genetic variation and environmental factors

d)

d) none of the above

7.

Genetic variation refers to:

a)

a) Differences in DNA sequences among individuals

b)

b) Environmental changes that affect gene expression

c)

c) Random mutations that occur during development

d)

d) None of the above

8.

The process represented in the diagram is most closely associated with the cell organelle known as the

a)

ribosome

b)

nucleolus

c)

mitochondrion

d)

chloroplast

9.
How is the information in a DNA molecule expressed in an organism?
a)
through plasmids
b)
through RNA polymerase
c)
through recombinant DNA
d)
through proteins
10.

Cells differentiate to perform different functions because

a)

all genes are turned on inside that cell

b)

all genes are turned off inside that cell

c)

different genes are turned on inside that cell

d)

different genes are turned on or off inside that cell

11.

During the process of differentiation

a)

all sections of DNA are activated to determine the structure of a body cell

b)

ONLY ONE section of DNA are deactivated to determine the structure of a body cell

c)

only specific sections of DNA sequence are activated to determine the structure and functions of a body cell

12.
DNA molecule segment is : TTACGCAAG
The mutated DNA segment is TTCGCAAG. This is an example of ___ mutation.
a)
Substitution
b)
Deletion
c)
Insertion
d)
Inversion
13.
Normal-
GAG-CTC-GAC-AGA
Mutant
GAG CTC-CAC-AGA
a)
no mutation
b)
substitution
c)
insertion
d)
deletion
14.

Which type of mutation is MOST LIKELY to cause the greatest disruption?

a)

Frameshift

b)

Silent

c)

Point

15.

Cell differentiation is critical during embryonic development. The process of cell differentiation results in the production of many different kinds of specialized cells. This process is most directly influenced by

a)

DNA

b)

Lipids

c)

Carbohydrates

d)

ATP

16.

What is the role of mRNA in expressing specialized structures?

a)

Making energy available for building specialized structures

b)

Serving as a template for build specialized proteins

c)

Bonding Amino Acids to form polypeptide chains

d)

Serving as a stored blueprint in the nucleus for building specialized proteins

17.

What is the role of stem cells in cell differentiation?

a)

They serve as a precursor for generalized cells

b)

These cells can turn into specialized cells and back into generalized cells when needed

c)

They are generalized cells that can lead to specialized cells

d)

They are specialized cells that can lead to generalized cells

18.

Which reason explains why nerve cells function differently than muscle cells?

a)

In nerve cells all the genes are expressed.

b)

In muscle cells all the genes are expressed.

c)

In both kinds of cells the same genes are expressed.

d)

In both kinds of cells different genes are expressed.

19.

From a single fertilized ovum undergoing a series of rapid cell divisions, a human infant develops. The embryonic cells become specialized for a variety of functions. Which of these statements best describes how different cell types develop?

a)

Each cell type contains only the active parts of the DNA needed for that cell type.

b)

Each cell has multiple copies of DNA that are affected in different ways by the environment to change the function of the cell at regular intervals.

c)

Each cell has an identical copy of DNA with enzymes controlling the expression of specific genes, leading to a variety of cells.

20.

The p53 gene stops the progression of the cell cycle when DNA damage is detected. Which of these is most likely to happen in an individual with a mutation in their p53 gene?

a)

Cell growth would be unregulated which could lead to tumor formation.

b)

Cells with these mutations would no longer undergo mitosis.

c)

The type of mutations that occur in daughter cells would decrease.

d)

The gene would not be replicated before cell division.

21.

Which statement best describes the importance of cell cycle checkpoints in preventing the development of cancerous cells?

a)

Checkpoints initiate mutations during DNA replication in the cell cycle.

b)

Checkpoints increase the rate at which the cells go through the cell cycle.

c)

Checkpoints allow the cell to correct errors as it continues through the cell cycle.

d)

Checkpoints give the cell time to assemble molecules needed for mitosis in the cell cycle.

22.
a)

The cells will continue to grow, causing a tumor in the body.

b)

The cells will replicate normally, replacing body cells that die.

c)

The cells will stop replicating once one layer is produced, as other body cells will crowd them out.

23.

What are onconogenes?

a)

genes that signal part of the protein synthesis cycle

b)

mutated forms of genes which can transform a cell into a tumor cell

c)

normal cells produced during mitosis

24.
What is a substance called if it speeds up a chemical reaction?
a)
reusable
b)
catalyst
c)
specific
d)
fragile
25.

Enzymes are an example of

a)

Lipids

b)

Carbohydrate

c)

Protein

d)

Nucleic Acid

26.
In the diagram, what is letter A? 
a)
product
b)
substrate
c)
enzyme-substrate complex
d)
active site
27.
Letter D...
a)
active site
b)
enzyme
c)
substrate
d)
products
28.
Letter B...
a)
active site
b)
enzyme
c)
substrate
d)
products
29.
Letter C...
a)
active site
b)
enzymes
c)
substrate
d)
products
30.
Letter E...
a)
active site
b)
enzyme
c)
substrate
d)
products
31.
An enzyme speeds up a reaction by
a)
lowering the activation energy.
b)
raising the activation energy.
c)
releasing energy.
d)
absorbing energy.
32.

When looking at the enzyme substrate model, the substrate turns into what at the end of the chemical reaction?

a)

Nothing

b)

It does not change

c)

Products

d)

Pieces

33.

What are two factors that can affect an enzymes ability to function?

a)

No changing

b)

temperature and pH

c)

night and day

d)

hot and cold

34.

How does an enzymes shape relate to its function?

a)

the shape of the enzyme has no relation to its function

b)

the enzyme is shaped specially for certain substrates to perform a specific reaction

c)

the enzyme shape acts as a receptor to certain products

d)

the enzyme shape can fit into specific substrates to cease a reaction

35.
The graph shows how enzymes affect reactions in cells. Which statement best summarizes the information shown in the graph?
a)
Enzymes increase the energy of the reactants.
b)
Enzymes decrease the energy level of the products.
c)
Enzyme decrease the activation energy of the reaction.
d)
Enzymes reverse the direction of the reaction.
36.

How many nucleotides make up a codon?

a)

1

b)

3

c)

5

d)

15

37.

What is the order for Protein Synthesis?

a)

Transcription -> Translation -> Protein

b)

Translation - > Transcription -> Protein

c)

Protein -> Transcription -> Translation

d)

Translation -> Protein -> Transcription

38.
How would the DNA sequence GCTATA be transcribed to mRNA?
a)
GCUAUA
b)
CGATAT
c)
CGAUAU
d)
GCUTUT
39.
Genes are found on...
a)
chromosomes
b)
alleles
c)
proteins
d)
anthers
40.
Nucleotides are made up of a sugar, phosphate, and..?
a)
nitrogenous base
b)
nucleic acid
c)
cholesterol
d)
fatty acid
41.

The information in DNA is written according to the genetic code and carried by the -

a)

sequence of nitrogen bases/nucleotides

b)

direction of the phosphate groups

c)

hydrogen bonds between nitrogen bases

d)

enzymes which unwind the double helix

42.

Characteristics such as a widow's peak or attached earlobes are determined by the genetic code. Which components of DNA are referred to as the genetic code?

a)

Phosphate groups

b)

Deoxyribose sugars

c)

Nitrogenous bases

d)

Hydrogen bonds

43.

DNA Replication results in

a)

One new strand and one original strand in one molecule of DNA

b)

Mirror image strands on one molecule of DNA

c)

One DNA molecule

d)

A completely new strand with different nucleotide base sequences

44.

In  Texas  Longhorn cattle,  coat coloration is an inherited trait.  What is the genetic basis of coat-color variation in  Texas  Longhorn cattle?

a)

Differences in the nucleotide sequences of genes 

b)

Differences in the numbers of chromosomes in cells 

c)

Differences in the diets of individual cattle

d)

Differences in the  environmental conditions of  different geographic areas

45.
What determines the code, or information, of a DNA molecule?
a)

the shape (structure) of the nitrogen bases of the nucleotide

b)

the order (sequence) of the nitrogen bases of the nucleotides

c)

the color of the nitrogen bases of the nucleotides

d)

the frequency (number) of nitrogen bases of the nucleotides

46.

Click to enlarge the picture of the model. Which explanation best describes the purpose of Step 1 in this process?

a)

To make an RNA copy of the DNA

b)

To transfer the amino acids to the polypeptide chain

c)

To catalyze the reaction that bonds amino acids together

47.

CRISPR-Cas9 is a genetic modification technique that edits parts of the genome of an organism. Using this technique scientists can add, remove, or modify sections of the DNA sequence.

How can scientists use this technique to control gene expression?

a)

By activating or deactivating specific genes

b)

By identifying how closely related one individual is to another

c)

By determining the number of chromosomes in an organism

48.

Click to enlarge photo. Use the information about the Rockcress plant from the table to answer the following:

What type of mutation MOST LIKELY occurred in the R gene that resulted in strong defenses against leaf-spot disease?

a)

deletion

b)

translocation

c)

substitution

d)

insertion

49.
What goes in box 3
a)
Aabb
b)
AABB
c)
AAbb
d)
aaBb
50.

What goes in box 15

a)

aabb

b)

AABB

c)

aaBB

d)

AAbb

51.
What is the genotype for a pea plant heterozygous for round seeds (R), and homozygous recessive for green seeds (y)?
a)
Ry
b)
RRyy
c)
RrYy
d)
Rryy
52.
Using the dihybrid cross and key provided, what is the probability of the offspring being tall and white?
a)
9
b)
2
c)
3
d)
6
53.

Based off this punnett square, what fraction of the offspring will have wrinkled, yellow seeds?

a)

9/16

b)

3/16

c)

1/16

d)

16/16

54.
Different forms of genes:
a)
alleles
b)
phenotype
c)
genotype
d)
homologous chromosomes
55.
An organism's appearance or other observable characteristics:
a)
phentoype
b)
genotype
c)
recessive
d)
dominant
56.
Having two of the SAME alleles for a trait (example HH or hh):
a)
homozygous
b)
heterozygous
c)
recessive
d)
dominant
57.
What does heterozygous mean?
a)
An organism that has the same genes for a trait (TT or tt)
b)
An organism that has different genes for a trait (Tt)
c)
When neither gene is fully dominant; offspring is a mixture of both
d)
the different forms of a trait (hair color--> brown, blonde, red
58.
An allele whose trait only shows up when no dominant allele is present.
a)
hidden allele
b)
dominant allele
c)
recessive allele
d)
present allele
59.
Two heterozygous (Tt)tall pea plants are crossed. Which describes their offspring?
a)
50% tall and 50% short
b)
75%tall and 25% short
c)
100%short
60.
What determines gender in humans? 
a)
the X and Y chromosome
b)
chromosome 21
c)
codominance
d)
epistasis
61.
Crossing over is important because....
a)
It prevents mutations from entering the gametes. 
b)
It allows for even distribution of chromosomes in the gametes.
c)
It allows for more genetic diversity of gametes.
d)
It allows for genetic uniformity of gametes. 
62.

Which law states that alleles for different traits separate independently during meiosis?

a)

Law of Independent Assortment

b)

Law of Segregation

c)

Law of Dominance

d)

Law of Crossing Over

63.

What kind of cells are made in MEIOSIS?

a)

Haploid Gametes

b)

Diploid Somatic Cells

c)

Skin Cells

d)

Liver Cells

64.
The process of crossing over occurs in what phase of meiosis?
a)
anaphase 1
b)
prophase 1
c)
prophase 2
d)
telophase 1
65.
The cells produced via meiosis are called:
a)
sex cells
b)
somatic cells
c)
body cells
d)
skin cells
66.
What causes genetic variation in meiosis?
a)
chromosomes lining up
b)
crossing over of chromosomes
c)
separation of chromosomes
d)
chromosomes pulling apart
67.

Look at the equations for photosynthesis and match the following

Categorize the following

Carbon Dioxide

Water

Glucose

Oxygen

CO2

H2O

C6H12O6

O2

reactants of photosynthesis
products of photosynthesis
68.

In the Law of Conservation of Mass/Energy they cannot be...

a)

Transferred & Transformed

b)

Created & Destroyed

c)

Moved & Changed

d)

Burned & Buried

69.

In photosynthesis, energy is ​ (a)   in carbon compounds (glucose contains carbon). In cellular respiration, energy is ​ (b)   from the carbon compounds to form ​ (c)  

Choose from the below words
stored
released
ATP energy