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Key Concepts in Genetic Engineering and Molecular Biology

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

A Restriction enzyme used to cut DNA at GAATTC Between the G and the 1st A and, leaves other four nucleotide bases exposed

a)

DNA polymerase

b)

Ligase

c)

Helicase

d)

ecoR1

2.

Using a high voltage electrical shock to bacteria so they suck plasmids through the pores in the membrane

a)

Chromosome

b)

Protein

c)

Electroporation

d)

Enzyme

3.

A process that uses laboratory techniques to alter the DNA of an organism

a)

Genetic Engineering

b)

Gene Silencing

c)

Natural Selection

d)

Spontaneous Mutation

4.

A plasmid or virus that is a vehicle to carry a DNA segment into a host cell

a)

Vector (as related to molecular biology)

b)

Endotoxin

c)

Virus particle

d)

Plasmodium

5.

Exposed nucleotide bases with strong polar charges that attract complementary nucleotide sequences.

a)

Guanine

b)

Sticky ends

c)

Adenine

d)

Cytosine

6.

Laboratory techniques to isolate and manipulate DNA segments

a)

Polymerase Chain Reaction (PCR)

b)

Gel Electrophoresis

c)

Genetic Engineering

d)

Fluorescent In Situ Hybridization (FISH)

7.

Organisms that have had DNA from other organisms inserted into their DNA to produce certain traits

a)

Autogenous creatures

b)

Natural-born species

c)

Transgenic organisms

d)

Homo sapiens evolved in isolation

8.

When fragments of DNA from different organisms are connected

a)

Homologous Sequence Alignment

b)

Genomic Duplication

c)

Chromosomal Crossover

d)

Recombinant DNA

9.

An antibiotic to which some bacteria have a natural resistance.

a)

Ciprofloxacin

b)

Ampicillin

c)

Penicillin

d)

Vancomycin

10.

An enzyme that chemically bonds two strands of DNA. It repairs breaks in DNA.

a)

Ribozyme

b)

Polymerase

c)

DNA ligase

d)

Helicase