Bacterial Transformation Reteach

Multiple Choice

How do the structural components of bacteria, such as the cell wall and nucleoid DNA, contribute to their ability to undergo transformation?

• The cell wall prevents DNA uptake, and the nucleoid DNA degrades foreign genes.
• The cell wall allows DNA uptake, and the nucleoid DNA facilitates genetic integration using plasmids.

• The cell wall restricts DNA absorption, and the nucleoid DNA impedes genetic exchange.
• The cell membrane blocks DNA entry, while plasmid DNA hinders integration.

Multiple Choice

Why is bacterial transformation considered a significant process in genetic engineering?

• It allows bacteria to reproduce faster

• It enables the introduction of new genes into bacteria

• It increases bacterial resistance to antibiotics

• It helps bacteria survive in extreme environments

Fill in the Blanks

Fill in the Blanks

Multiple Choice

Which structure in a bacterial cell is responsible for movement?

• Flagellum

• Pili

• Capsule

• Ribosomes

Multiple Select

Select all the functions of the bacterial capsule.

• Protects bacteria from toxic compounds

• Helps bacteria adhere to surfaces

• Main source of DNA

• Makes proteins

Multiple Choice

Which of the following statements best describes the role of plasmids in bacteria?

• Plasmids carry genes that often give bacteria a genetic advantage.

• Plasmids are the main source of DNA in bacteria.

• Plasmids make proteins for the cell.

• Plasmids maintain cell shape.

Open Ended

Explain how plasmids contribute to genetic transformation in bacteria.

Multiple Choice

Which enzyme is used to cut both plant DNA and plasmid DNA in the process shown?

• Ligase

• Restriction enzyme

• Polymerase

• Helicase

Multiple Choice

Explain why it is important for the target gene to be inserted in the correct orientation in a plasmid for bacterial expression.

• Correct orientation is only necessary for plasmid stability.
• Inserting the gene in any direction will enhance bacterial growth with the incorrect trait being replicated.

• It is important for the target gene to be inserted in the correct orientation to ensure proper transcription and translation for effective bacterial expression.
• The orientation of the target gene does not affect protein synthesis.

Open Ended

Describe the steps involved in producing recombinant insulin as shown in the diagram.

Multiple Choice

Which organism is the original source of Green Fluorescent Protein (GFP)?

• Jellyfish

• Bacteria

• Corn

• Fish

Multiple Choice

GFP is used to identify ___ organisms.

• transgenic
• artificial

• natural

• incorporated

Multiple Choice

Which of the following are applications of GFP as a visual marker?

• Study of protein synthesis

• Cell movement

• Formation of organs

• All of the above

Open Ended

Explain how GFP can be used as a visual tracer or marker in biological research.

Open Ended

What are some examples of genetic engineering in agriculture and medicine?

Multiple Choice

What is the function of the 'ori' region in a plasmid?

• It allows bacteria to make copies of the plasmid.

• It codes for a protein.

• It is used for transcription.

• It is the site for translation.

Multiple Select

Which of the following statements about genetic transformation are correct?

• Genetic transformation occurs when a cell takes up DNA and expresses the genes on that DNA.

• Only bacteria can be genetically transformed.

• Genetic transformation cannot occur in plants.

• Many types of cells, including human cells, can be transformed.

Open Ended

Explain how a gene cloned into a plasmid can lead to protein production in bacteria.

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Multiple Choice

Which is the correct sequence for the process of genetic transformation?

• bacterial cells transferred to cold CaCl₂ , plasma with foreign DNA added bacteria , cells heat shocked at 42^oC , cells spread on agar petri dish , bacteria given time to grow on petri dish

• bacterial cells transferred to cold CaCl₂ , cells heat shocked at 42^oC , plasma with foreign DNA added bacteria , bacteria given time to grow on petri dish , cells spread on agar petri dish

• plasma with foreign DNA added bacteria , bacterial cells transferred to cold CaCl₂ , cells spread on agar petri dish , bacteria given time to grow on petri dish , cells heat shocked at 42^oC