2-8-2023

Distinguish between leading and lagging strand.

Answer explanation

A. Leading strand moves continuously towards replication fork. One replication fork.

B. Lagging strand moves discontinuously away from the replication fork thereby creating Okazaki fragments that are linked together by ligase. Multiple replication forks

How are the leading strand and lagging strand in DNA replication similar?

Answer explanation

Both add new nucleotides in the 5' ---> 3' direction

Both require an RNA primer for DNA polymerase to begin replication

Which order is the correct sequence of DNA replication?

   I. Okazaki fragments are joined

  II. separation of DNA strands

  III. complimentary nucleotides are added

  IV. primers are added

• I, II, III, IV

• II, IV, III, I

• II, I, IV, III

• IV, II, III, I

What is a VNTR and what is it used for?

Answer explanation

Variable number of tandem repeats are used to compare DNA between individuals

Which sequence of DNA would be suitable in DNA profiling?

  A.   ---ATTCGTGAATCAGCC---

  B.   ---ATTCGTGAATTTGCC---

  C.   ---ATTCGTGATTGCAGC---

  D.   ---ATTCGTGATTCGTGA--- 

• ATTCGTGAATCAGCC

• ATTCGTGAATTTGCC

• ATTCGTGATTGCAGC

• ATTCGTGATTCGTGA

Answer explanation

ATTCGTGATTCGTGA

Answer D is the only one with a DNA sequences that repeats in a continuous fashion

Ture-false. If you look at the DNA matches between a baby and a potential father for 10 different VNTR alleles, if 9 of the 10 match, then the potential father is the father of the child.

• True

• False

Answer explanation

False. All you need is one of the VNTR tests from the potential father to not match the child to disqualify that man as the father.