Grade 13 Biology NGS Quiz

What are the main steps in the workflow of next generation sequencing?

• RNA isolation, reverse transcription, cDNA amplification, and Sanger sequencing

• DNA extraction, PCR amplification, gel electrophoresis, and DNA sequencing

• Library preparation, cluster generation, sequencing, and data analysis

• Cell lysis, protein purification, mass spectrometry, and data analysis

Explain the process of library preparation in next generation sequencing.

• Fragmenting proteins, adding adapters, amplifying DNA, and purifying the library

• Fragmenting DNA, adding adapters, amplifying DNA, and purifying the library

• Digesting RNA, adding primers, amplifying RNA, and purifying the library

• Copying DNA, adding adapters, amplifying RNA, and purifying the library

Describe the sequencing step in next generation sequencing.

• Generation of millions of short DNA sequences in parallel

• Manual extraction of long DNA sequences

• Sequencing of only one DNA sequence at a time

• Generation of RNA sequences instead of DNA

What is the role of bioinformatics in next generation sequencing workflow?

• Bioinformatics only focuses on experimental design

• Bioinformatics is not relevant in next generation sequencing workflow

• Bioinformatics helps in collecting the samples

• Bioinformatics helps in analyzing and interpreting the data

How does next generation sequencing data analysis differ from traditional sequencing methods?

• Next generation sequencing data analysis involves high-throughput methods and bioinformatics tools to handle large volumes of data, while traditional sequencing methods are slower and less efficient.

• Next generation sequencing data analysis does not handle large volumes of data

• Next generation sequencing data analysis uses manual methods and does not involve bioinformatics tools

• Traditional sequencing methods are faster and more efficient than next generation sequencing data analysis

Compare the throughput of Sanger sequencing and next generation sequencing.

• Polymerase chain reaction

• DNA microarray

• Next generation sequencing

• Sanger sequencing

Discuss the cost-effectiveness of Sanger sequencing versus next generation sequencing.

• Next generation sequencing is more cost-effective for small-scale projects

• Sanger sequencing is always more cost-effective than next generation sequencing

• Sanger sequencing is more cost-effective for small-scale projects, while next generation sequencing is more cost-effective for large-scale projects.

• Sanger sequencing is more cost-effective for large-scale projects

Explain the accuracy and error rate in Sanger sequencing and next generation sequencing.

• Sanger sequencing has high error rates and limited read length

• Next generation sequencing has high accuracy and limited read length

• Sanger sequencing has low error rates but can generate large amounts of data quickly

• Sanger sequencing has high accuracy but limited read length, while next generation sequencing has higher error rates but can generate large amounts of data quickly.

How does the speed of Sanger sequencing compare to next generation sequencing?

• Sanger sequencing is faster

• Next generation sequencing is faster

• Both Sanger sequencing and next generation sequencing have the same speed

• Sanger sequencing is more accurate than next generation sequencing