Biotechnology Quiz: Tools Used in Genetic Engineering

What is the basic physical and functional unit of heredity?

• Genes

• Alleles

• Ribonucleic acid

• Deoxyribonucleic acid

Which branch of science is concerned with how various traits are inherited?

• Biological engineering

• Genetics

• Pathology

• Microbiology

What process utilizes recombinant DNA technology to alter the genetic makeup of an organism?

• Genetic modification

• Biological engineering

• Gene therapy

• Genetic engineering

Who isolated the first restriction enzyme?

• Kary Mullis

• Hindll

• James Watson

• Ian Wilmut

Which organism was the first used in DNA technology experiments?

• S. typhimurium

• E. coli

• S. cerevisiae

• E. faecalis

What laboratory technique is used to amplify a segment of DNA?

• DNA ligase

• Restriction endonuclease

• Polymerase chain reaction

• Gel electrophoresis

What are enzymes that cut DNA at specific locations called?

• DNA polymerase

• Restriction enzymes

• Primase

• Helicase

Which enzyme is responsible for creating covalent bonds between nucleotide chains?

• DNA polymerase

• Restriction enzyme

• RNA polymerase

• DNA ligase

What is the most commonly used eukaryotic organism in gene cloning?

• E. coli

• Mus musculus

• Saccharomyces cerevisiae

• Drosophila melanogaster

What is the purpose of gel electrophoresis?

• To amplify DNA

• To visualize DNA fragments

• To cut DNA

• To ligate DNA