PCR and Gel Electrophoresis

Front

What is PCR?

Back

PCR (Polymerase Chain Reaction) is a technique used to amplify small segments of DNA, making millions of copies of a specific DNA sequence.

Front

How many copies of DNA are made after 1 cycle of PCR?

Back

2 copies.

Front

What is the role of a restriction enzyme in DNA analysis?

Back

Restriction enzymes cut DNA at specific sequences, allowing for the analysis of DNA fragments.

Front

If a restriction enzyme makes one cut in the gene, how many bands will appear on the gel?

Back

2 bands.

Front

What is gel electrophoresis?

Back

Gel electrophoresis is a laboratory method used to separate DNA fragments based on their size.

Front

What is the purpose of gel electrophoresis?

Back

To separate DNA based on size.

Front

What force separates DNA fragments during gel electrophoresis?

Back

Electrical force.

Front

Why do DNA fragments travel towards the positive electrode in gel electrophoresis?

Back

DNA is negatively charged, so it is attracted to the positive end of the unit.

Front

What is the significance of the size of DNA fragments in gel electrophoresis?

Back

Smaller DNA fragments move faster and travel further in the gel than larger fragments.

Front

What is agarose gel made from?

Back

Agarose gel is made from agarose, a substance derived from seaweed, used to create a matrix for DNA separation.