MI

Why are you not supposed to boil the GFP native solution?

• we want to denature it

• it is already denatured

• it will help us compare native and denatured proteins after electrophoresis

Place the following in order for SAFE recovery of your completed gel.

• remove the gel

• stain the gel

• turn off the power supply

• disconnect the leads

• remove the cover

Which of the following are important guidelines for working with bacteria in this lab? (Check all that apply.)

• wipe down tabletops with disinfectant before and after lab

• disinfect all materials that contact bacteria before disposing

• wash hands with soap before and water after lab work

• know the protocol BEFORE attempting any tasks

The purpose of Module 1 is to _______ the E. coli bacteria with the pGFP plasmid.

Which letter represents amplitude? (a)  

• A

• B

• C

• D

• E

Click on the supernatant

Tags

NGSS.MS-PS1-2

NGSS.MS-PS1-5

On which plate(s) do you expect to see growth of GLOWING colonies? (Choose all that apply.)

• -DNA

• -DNA/+Amp

• +DNA/+Amp

• +DNA/+Amp/+IPTG

Which term is closest in meaning to lysis?

• rupture

• isolate

• separate

• express

• grow

The chromatography column will slowly release liquid, drop-by-drop. How will you know that you have a drop containing GFP?

• it will come out first

• it will come out last

• it will glow under UV light

• it will appear yellow

Tags

NGSS.HS-PS4-5

How will you denature the proteins?

• boil them

• scare them

• apply electricity

• use lysis buffer