Recombinant Plasmids and Human Insulin

What is a recombinant plasmid?

• A plasmid that has been naturally formed in bacteria.

• A plasmid that has been genetically engineered to include foreign DNA.

• A plasmid that is resistant to antibiotics.

• A plasmid that can replicate independently of the bacterial chromosome.

What is the primary purpose of using plasmids as vectors in genetic engineering?

• To increase the size of bacterial cells.

• To introduce foreign DNA into bacterial cells.

• To make bacteria resistant to viruses.

• To enhance the natural plasmid replication process.

What role do restriction enzymes play in the creation of recombinant plasmids?

• They synthesise new DNA strands.

• They cut DNA at specific sequences.

• They replicate plasmids within bacterial cells.

• They repair damaged DNA.

Why is it important to include a selectable marker gene in a plasmid vector?

• To ensure the plasmid can replicate in the host cell.

• To allow identification of cells that have taken up the plasmid.

• To increase the size of the plasmid.

• To prevent the plasmid from being degraded.

Which of the following is a common method for introducing recombinant plasmids into bacterial cells?

• Electroporation

• Centrifugation

• Filtration

• Precipitation

What is the significance of using bacterial cells in the production of human insulin?

• Bacteria can produce insulin more efficiently than human cells.

• Bacteria can be easily manipulated to produce large quantities of insulin.

• Bacteria naturally produce human insulin.

• Bacteria are immune to insulin degradation.

Which of the following is a benefit of using recombinant DNA technology to produce human insulin?

• It reduces the cost of insulin production.

• It eliminates the need for animal insulin.

• It increases the purity and safety of insulin.

• All of the above.

What is the role of ligase in the creation of recombinant plasmids?

• To cut DNA at specific sites.

• To join DNA fragments together.

• To replicate DNA.

• To degrade unwanted DNA.

Which enzyme "glues" DNA fragments together during insertion?

• restriction enzymes

• DNA ligase

• DNA polymerase

• DNA helicase

What type of cut is this photo?

• Blunt

• sticky