Front

What is the gene of interest in biotechnology?

Back

The specific gene that researchers want to replicate or express.

Front

What does PCR stand for and what is its primary use?

Back

PCR stands for Polymerase Chain Reaction, and it is used to amplify DNA.

Front

What is the role of RNA polymerase during transcription?

Back

RNA polymerase binds to the promoter and synthesizes a complementary RNA strand.

Front

At which stage of mitosis do sister chromatids separate?

Back

Sister chromatids separate during Anaphase.

Front

Define molarity in the context of solutions.

Back

Molarity is defined as moles of solute per liter of solution.

Front

What is the purpose of cloning a gene?

Back

To replicate or express the gene for further study or application.

Front

What is the significance of the promoter in gene expression?

Back

The promoter is a DNA sequence that initiates transcription by providing a binding site for RNA polymerase.

Front

What is the difference between DNA and RNA?

Back

DNA is double-stranded and contains deoxyribose sugar, while RNA is single-stranded and contains ribose sugar.

Front

What is the function of tRNA in protein synthesis?

Back

tRNA brings amino acids to ribosomes during translation.

Front

What occurs during the process of transcription?

Back

The DNA sequence of a gene is transcribed into a complementary RNA strand.