MVI_G1: Acara 4

DNA is … so it will move from …

• Negatively charged, anode to cathode
• Negatively charged, cathode to anode
• Positively charged, anode to cathode
• Positively charged, cathode to anode

Agarose gel’s form is…

• Disaccharides
• Monosaccharide
• Polysaccharides
• Oligosaccharides

The voltage used for TAE is…

• 100 V
• 200 V
• 1 V
• 20 V

Ethidium Bromide requires careful handling because its compounds are …

• Mutagenic
• Explosive
• Safe
• Sensitive

High concentration gel will cause …

• Slower DNA migration
• Faster DNA migration
• Higher voltage
• Lower voltage

Aris has 50 ml TBE 1X. To make a 3% agarose gel, he should add … grams of agarose powder.

• 0,5
• 5.0
• 1,5
• 0,1

Based on the picture, the material is …

• TBE Buffer
• DNA sample
• Agarose powder
• DNA standard

After sample loading, the next step is …

• Sample running for 20 minutes with 50 V
• Visualization with 2UV Transilluminator Black Side
• Sample running for 20 minutes with 100 V
• Visualization with 2UV Transilluminator White Side

If the DNA sample is 9 µl, the GBB is … µl

• 3

• 2

• 9

• 6

Glycerin is used as …

• Tracking Dye
• DNA ballast
• Bromphenol Blue
• DNA stain