Quiz on Gel Electrophoresis and PAGE

What does PAGE stand for?

• Polymer Gel Electrophoresis

• Polyacrylate Gel Electrophoresis

• Polymerase Gel Electrophoresis

• Polyacrylamide Gel Electrophoresis

Who reported the moving boundary method of electrophoresis?

• James Watson

• Albert Einstein

• Arne Tiselius

• Marie Curie

What is the primary purpose of electrophoresis?

• To heat proteins

• To purify water

• To synthesize DNA

• To separate molecules based on their properties

Which factor does NOT affect the rate of migration in electrophoresis?

• Net charge

• Color of the gel

• Buffer viscosity

• Mass and shape

What is the role of SDS in SDS-PAGE?

• To denature proteins

• To increase pH

• To stabilize the gel

• To provide nutrients

In which condition does Native PAGE operate?

• Low pH

• Non-denaturing conditions

• Denaturing conditions

• High temperature

What is the main difference between continuous and discontinuous buffer systems?

• Temperature settings

• Type of samples

• Number of gels used

• Color of the gel

What is the purpose of using a stacking gel in a discontinuous system?

• To change the pH

• To stabilize the temperature

• To decrease sample size

• To increase resolution

Which of the following is a common method for staining proteins in gels?

• Magnetic Staining

• Coomasie Blue Staining

• Fluorescent Staining

• Radioactive Staining

What is the main component used to form polyacrylamide gels?

• Cellulose

• Acrylamide

• Agarose

• Gelatin