DNA Extraction

What is the main purpose of Proteinase K in DNA extraction?

• A. Binding DNA to silica

• B. Breaking down proteins

• C. Dissolving membranes

• D. Precipitating DNA

Which combination best promotes DNA precipitation during extraction?

• A. SDS and proteinase K

• B. TE buffer and ethanol

• C. Absolute ethanol and salt

• D. Isopropanol and lysozyme

In phenol-chloroform extraction, where is DNA located after centrifugation?

• A. In the organic phase

• B. In the interface

• C. In the aqueous phase

• D. Bound to silica

Why is EDTA used in the elution buffer?

• A. To dissolve proteins

• B. To maintain alkaline pH

• C. To deactivate nucleases

• D. To precipitate DNA

What is the major challenge of using silica column kits?

• A. Contamination with organic solvents

• B. Inability to process large DNA

• C. Loss of small DNA fragments

• D. Time-consuming procedures

Centrifugation is only used in organic extraction methods.

• True

• False

Guanidine salts act as chaotropes to help lyse cells chemically.

• True

• False

The wash buffers used in column purification contain 70% alcohol.

• True

• False

Lysozyme is used to digest proteins during DNA extraction.

• True

• False

GeneJET™ kits use organic solvents like phenol and chloroform.

• True

• False