TCA Cycle Regulation

The video tutorial explores the regulation of the TCA cycle, focusing on allosteric enzymes and effectors. It discusses the role of pyruvate dehydrogenase, citrate synthase, isocitrate dehydrogenase, and alpha ketoglutarate dehydrogenase in the cycle. The tutorial explains how high and low energy charge molecules, such as ATP and NADH, influence enzyme activity. Additionally, it covers the phosphorylation of pyruvate dehydrogenase and its impact on enzyme regulation.

What is the role of pyruvate dehydrogenase in the TCA cycle?

Describe the allosteric effectors that influence pyruvate dehydrogenase.

How does the presence of free fatty acids influence pyruvate dehydrogenase activity?

How does ATP affect the activity of citrate synthase?

What is the significance of calcium ions in the regulation of isocitrate dehydrogenase?

What are the main allosteric inhibitors of alpha ketoglutarate dehydrogenase?

Discuss the role of acetyl CoA in metabolism.

Explain the feedback inhibition mechanism involving NADH in the TCA cycle.

What is the relationship between pyruvate dehydrogenase and energy charge?

What are the consequences of high energy charge molecules on the TCA cycle enzymes?