WorksheetsBiotech Chapt 6
Total questions: 45
Worksheet time: 34mins
Name
Class
Date
1.
A highly specific test to determine protein concentration based on an antigen-antibody interaction
a)
concetration assay
b)
ELISA
c)
potency assay
d)
stability assay
2.
Shows if a specific protein is present and conducting the expected reaction
a)
concetration assay
b)
ELISA
c)
potency assay
d)
stability assay
3.
Helps researchers measure the amount of protein present
a)
concetration assay
b)
PK/PD assay
c)
toxicology assay
d)
stability assay
4.
Determines the amount and length of activity for a given protein in a test organism
a)
activity assay
b)
PK/PD assay
c)
toxicology assay
d)
stability assay
5.
Used for understanding the relationship between dosage and activity of a given drug
a)
concetration assay
b)
PK/PD assay
c)
potency assay
d)
stability assay
6.
A way to determine how long the pharmaceutical is effective and how it should be stored
a)
activity assay
b)
PK/PD assay
c)
toxicology assay
d)
stability assay
7.
Used to determine what are safe and unsafe levels of a given drug
a)
concetration assay
b)
ELISA
c)
toxicology assay
d)
stability assay
8.
The use of a single antibody with an enzyme reporter to recognize an antigen
a)
direct ELISA
b)
indirect ELISA
c)
transfection
d)
formulation
9.
When mammalian cells incorporate rDNA and express new genes
a)
direct ELISA
b)
indirect ELISA
c)
transfection
d)
formulation
10.
The delivery method for a pharmaceutical product
a)
formulation
b)
transfection
c)
assay
d)
antibiotic
11.
A test for determining the presence or a particular characteristic of a protein
a)
formulation
b)
transfection
c)
assay
d)
antibiotic
12.
Molecules, produced by bacteria or fungus, which inhibit bacterial growth
a)
formulation
b)
transfection
c)
assay
d)
antibiotic
13.
The use of primary and secondary antibodies with an enzyme reporter to recognize an antigen
a)
direct ELISA
b)
indirect ELISA
c)
assay
d)
antibiotic
14.
Phase of the product pipeline where the purification process for rDNA is determined
a)
research and development
b)
manufacturing
c)
clinical trials
15.
Phase of the product pipeline where toxicity and efficacy studies are performed on mice
a)
research and development
b)
manufacturing
c)
clinical trials
16.
Phase of the product pipeline where safety and efficacy studies on human subjects are monitored by the FDA
a)
research and development
b)
manufacturing
c)
clinical trials
17.
Phase of the product pipeline where large volume production of the drug occurs
a)
research and development
b)
manufacturing
c)
clinical trials
18.
Phase of the product pipeline where a potential product is identified
a)
research and development
b)
manufacturing
c)
clinical trials
19.
Phase of the product pipeline where host cells get transformed and gene regulation is studied
a)
research and development
b)
manufacturing
c)
clinical trials
20.
Phase of the product pipeline where assays are developed to determine presence and activity of the drug
a)
research and development
b)
manufacturing
c)
clinical trials
21.
What is amylase?
a)
an enzyme
b)
a sugar
c)
a polysaccharide
d)
a starch
22.
What is the function of amylase?
a)
degrades plant starch into maltose
b)
degrades disaccharides into glucose
c)
bonds with other starches to form plant cell walls
d)
artificial sweetener
23.
Which of the following is an enzyme commonly used as a reported in ELISA or Western blots?
a)
Amylase
b)
Maltose dehydrogenase
c)
Tetramethylbenzene
d)
Horseradish peroxidase
24.
ELISAs are frequently used to assay for proteins during the manufacturing because
a)
they are very general
b)
they are very specific
c)
only occasional samples need to be taken
d)
a simple indicator test is sufficient
25.
All of the following are involved in Western blots except
a)
primary antibody
b)
secondary antibody
c)
substrate
d)
colloid suspensions
26.
How does an ELISA work in home pregnancy tests?
a)
it detects HCGH from urine using an anti-HCGH antibody
b)
it detects anti-HCGH antibody using HCGH hormone
c)
It detects HGH from the fetus
d)
It detects the HGH from urine using an anti-HGH hormone
27.
Amylase is
a)
a protein that converts starch into maltose
b)
a protein that converts starch to glucose
c)
a carbohydrate composed of maltose
d)
a carbohydrate composed of glucose
28.
A starch test was performed on 4 samples. A was light brown, B was dark blue, C was light blue, and D was medium blue. Which sample exhibited the most amylase activity?
a)
A
b)
B
c)
C
d)
D
29.
What assay should be done to test for the presence an concentration of a specific antigen through the research and manufacturing process?
a)
activity assay
b)
ELISA
c)
potency assay
d)
stability assay
30.
Herbal therapies have been used in some cultures for hundreds of years, but caution should be taken for all of these reasons, EXCEPT
a)
they're not subject to FDA approval
b)
adverse effect may occur from the wrong dose
c)
they taste better
d)
certain combos could be harmful
31.
In a pregnancy test, if HCGH is present in urine a pink line is visible. What kind of assay is this?
a)
activity assay
b)
concentration assay
c)
biuret test
d)
indicator test
32.
A new product is found to kill 92% of lymphoma in rats. What step of CPDP does this address?
a)
patient protection
b)
market is large enough
c)
preliminary data support efficacy
d)
safety of the drug is assured
33.
Protein therapeutics or produced in bioreactors of E. coli or CHO cells because
a)
there is not enough source material
b)
patients don't like being injected with "animal parts"
c)
plant and animal genes are incompatible with humans
d)
FDA stopped approving plant and animal sources
34.
Which of the following is an indicator used in protein concetration determinations?
a)
ELISA indicator
b)
BPA solution
c)
Bradford reagent
d)
BCIP
35.
What assay would a scientist use to determine the shelf life of a drug?
a)
activity assay
b)
ELISA
c)
PK/PD
d)
stability assay
36.
If the concentation of lactase needed to be measured using Bradford reagent, which instrument would be used?
a)
thermocycler
b)
spectrophotometer
c)
fermenter
d)
capillary detector
37.
Why is the destruction of rain forests concerning to pharmaceutical scientists?
a)
antimicrobials are hard to find in temperate regions
b)
plant purification steps will need to be performed in a lab
c)
destruction of forests will hasten global warming
d)
plant and animal sources of potential new drugs are being wiped out
38.
A tech sees a 55 kDA band on a PAGE gel and suspects it's amylase. How can she confirm it is?
a)
Eastern blot
b)
Northern blot
c)
Southern blot
d)
Western blot
39.
In an iodine amylase test, Sample A is dark blue, Sample B is light brown, and Sample C is faint blue. Which contains more active amylase?
a)
A
b)
B
c)
C
40.
Bradford reagent may be used to determine
a)
molecular weight
b)
concentration of protein
c)
activity of amylase
d)
all of the above
41.
What organism can naturally produce amylase?
a)
cat
b)
algae
c)
fungi
d)
dog
42.
Which of the following can be used as a Western blot membrane?
a)
agarose
b)
polyacrylamide
c)
nitrocellulose
d)
filter paper
43.
ELISA technology is used in all of the following except
a)
producing primers and probes
b)
recognizing contaminet protein
c)
testing for disease-causing agents
d)
measuring the amount of protein produced
44.
Recombinant DNA
a)
is used to produce 100's of other DNA products
b)
can be found in fungus cells
c)
is produced by pasting DNA from 2 sources together
d)
is larger than a typical chromosome
45.
Membranes used during Western blots are made of either PVDF or
a)
agarose
b)
agar
c)
nitrocellulose
d)
polyacrylamide
100 %
