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WorksheetsGeneral Microbiology
Total questions: 60
Worksheet time: 30mins
Most microorganisms pathogenic for humans grow best in the laboratory when cultures are incubated at
15–20°C
20–30°C
30–37°C
38–50°C
50–55°C
Which of the following is NOT assimilated by eukaryotic cells?
Glucose
Lactate
Sulfate
Nitrogen
Phosphate
Which of the following terms best describes a microorganisms that grows at 20°C?
Neutralophile
Psychrotroph
Mesophile
Osmophile
Thermophile
Which of the following is NOT a mechanism for generating metabolic energy by microorganisms?
Fermentation
Protein synthesis
Respiration
Photosynthesis
C and D
Polymerization of building blocks (eg, amino acids) into macromolecules (eg, proteins) is achieved largely by
Dehydration
Reduction
Oxidation
Assimilation
Hydrolysis
The major effect of a temperature of 60oC on the growth of a mesophile such as Escherichia coli is to
Destroy the cell wall
Denature proteins
Destroy nucleic acids
Solubilize the cytoplasmic membrane
Cause formation of endospores
The process by which microorganisms form ATP during the fermentation of glucose is characterized by
Coupling of ATP production with the transfer of electrons
Denitrification
The reduction of oxygen
Substratephosphorylation
Anaerobic respiration
Mutations in bacteria can occur by which of the following mechanisms?
Base substitutions
Deletions
Insertions
Rearrangements
All of the above
The form of genetic exchange in which donor DNA is introduced to the recipient by a bacterial virus is
Transformation
Conjugation
Transfection
Transduction
Horizontal transfer
The enzyme DNAse degrades naked DNA. If two strains of bacteria from the same species were mixed in the presence of DNAse, which method of gene transfer would be most likely inhibited?
Conjugation
Transduction
Transformation
Transposition
All of the above
Why do bacteria contain restriction enzymes?
To cleave RNA for incorporation into ribosome
To extend the length of bacterial chromosomes
To prevent foreign DNA from incorporating into a bacterial genome
To process the exons from prokaryotic mRNA
To proteolytically cleave nuclear promoters
Replication of which of the following requires physical integration with a bacterial replicon?
Single-stranded DNA bacteriophage
Double-stranded DNA bacteriophage
Single-stranded RNA bacteriophage
Plasmid
Transposon
The central autoclave for sterilization received nutrient media with substances that change at temperatures above 100˚C, media containing native proteins, Petri dishes, pipettes. Which method of sterilization would you choose for environments with substances that change at temperatures above 100˚C?
Chemical
Boiling
Dry heat
Current steam
Steam under pressure.
In pharmaceutical practice are widely used: asepsis, antiseptics, disinfection, sterilization. Give the correct definition of the term "asepsis".
Use of substances that kill microorganisms on the skin and mucous membranes
Destruction of pathogenic microbes in the environment
Complete destruction of all forms of microbes in an object
Prevention of microbes entering an object
Use of substances that kill pathogenic microbes in the internal environment of the body
In the fight against infectious diseases and for their prevention, disinfection is of great importance. What should be understood by this term?
Destruction of microorganisms in the environment
Destruction of pathogens in the internal environment
the patient’s body
Destruction of pathogens on the surface of the skin
and mucous membranes
Destruction of insect vectors of infectious agents
Killing rodents - sources of infection
Immunofluorescence response is widely used for the rapid diagnosis of many bacterial and viral infections. Select a condition that cannot be determined by the response.
Serum of the patient
The presence of an electron microscope
The presence of an immersion microscope
Selected pure culture of the pathogen
The presence of a fluorescent microscope
In the laboratory for the purpose of accelerated sterilization of sugar-containing media with liquid steam, it was carried out in one day: in the morning, afternoon and evening for 30 minutes. How should the environment be sterilized?
Sterilize for 1 hour
Sterilize for 15 minutes
Sterilize for 45 minutes
The medium should be sterilized three times with an interval of 24 hours
Sterilize twice a day
Which method of sterilization should be used for dental instruments that do not deteriorate from the effects of temperature and humidity for the guaranteed destruction of viruses, vegetative and spore forms of microorganisms?
Tyndalization
Pasteurization
Glowing over a gas burner flame
Autoclaving
Boiling
The dentist performed tooth extraction in an HIV-infected person. During this manipulation, the patient's blood got on the doctor's skin. What is the most appropriate treatment for skin disinfection?
Chloramine
Hydrogen peroxide
Ethyl alcohol
Formalin
Lysol
In microbiology practical training students are offered to paint a mixture of bacteria using the Gram method and to explain the mechanism of staining. What are the morphological structures of bacteria that cause gram-negative and gram-positive staining of bacteria?
Cell wall
Capsule
Flagella
Cytoplasm
After completing the work in the educational bacteriological laboratory, the student must tidy up his workplace, decontaminate the table and other objects. What chemical compound should he use for this?
Chloramine.
Hydrochloric acid.
Formalin.
Chloroform.
Ether.
The laboratory carried out studies in which the patient was confirmed diagnosis of tetanus. Which method of sterilization should eliminate the isolated cultures of tetanus?
Pasteurization.
Boiling.
Induction.
Dry heat.
Autoclaving.
In the bacterioscopic method of laboratory diagnostics of infections, various methods of staining of microscopic preparations are used. What is the purpose of using the Gram method?
For the detection of capsules
To color the dispute
For bacterial differentiation
To detect flagella
To detect plasmids
Immunofluorescence reaction is widely used for the rapid diagnosis of many bacterial and viral infections. Choose a condition without which it is impossible to determine the result of the reaction.
The presence of a luminescent microscope
The presence of an electron microscope
Presence of an immersion microscope
The selected pure culture of the pathogen
Serum sick
With many infectious diseases in the patient's blood, antigens of the pathogen can be detected. What reaction should be applied, given that the level of antigenemia is low?
Solid-phase immuno-fluorescence analysis
Reaction to indirect hemagglutination
Agglutination reaction
The reaction of latex agglutination
Immunoelectrophoresis
In recent years, has found an application of the method of genoindication of pathogens, which allows detecting fragments of nucleic acids of pathogens in the samples under study. Choose from the reactions that are appropriate for this purpose.
Polymerase chain reaction
A reaction of titer phage growth
Radioimmunoassay
Reaction of precipitation
Immunoenzyme analysis
Serologic diagnosis of infectious diseases is based on the specific interaction of antibodies with antigens. What is the name of a serological reaction in which antibodies are used, labeled with fluorescents?
Immunofluorescence reaction
The reaction of enzyme-labeled antibodies
Chain polymerase reaction
Chain polymerase reaction
The reaction of molecular hybridization
Wide proliferation in virological studies has received ELISA. For what purpose in the diagnosis of viral infections IFA is used:
Identification of the complement
To detect specific antibodies
To determine the antibody titer
To isolate the virus
To determine the virions assimilated by immune serum
High sensitivity and specificity of the radioimmunological method. For what purpose use this method:
To detect microorganism’s antigens
To titrate toxins and toxins
To detect toxoid
To detect enzymes
To detect allergens
Direct response of immunofluorescence is widely used in the diagnosis of infectious diseases. Indicate why this reaction is applied:
To identify pathogens of infectious diseases
To study defective viruses
To determine the type and level of antibodies in the serum of the patient's blood
To determine the specificity of blood
To study cellular immunity and differentiate the population of B-lymphocytes from other lymphoid cells
The immunofluorescence reaction is among the widely used for the purpose of rapid diagnosis of infectious diseases. What are the properties of bacteria taken into account for their identification under conditions of use of this reaction?
Morphological and antigenic
Morphological and tinctorial
Antigenic and immunogenic
Cultural and antigenic
Cultural and enzymatic
The disadvantage of the microscopic method of diagnosing infectious diseases is its insufficient in formativeness due to the morphological similarity of many types of microorganisms, which immunological reaction allows precisely to determine the type of pathogen:
reaction of precipitation
immunoblotting
Cumbus reaction
opsonization reaction
immunofluorescence reaction
The method of fluorescing antibodies is widely used for the rapid diagnosis of most viral infections. What can be detected using RIF:
Antibodies (IgM) in the serum of the patient
Incomplete antibodies
The growth of titer of antibodies in the blood serum of the patient
Antibodies (IgA) in the serum of the patient's blood
Number of DNA viruses
For the study of saccharolytic properties, the isolated pure culture of the microbes was seeded in semi-liquid agar tubes containing the indicator and carbohydrates - glucose, lactose, mannitol, sucrose. What are the environments used in this case?
Hissa
Ressel
Loeffler
Endo
Lowenstein – Jensen
What are the nutrient media best to do the primary sowing when isolating a pure bacterial culture from a complex microbial mixture:
Differential-diagnostic.
Simple.
Special.
Elective.
Canning.
Nutrient media are used for the cultivation of microorganisms. Some of the media contain substances that inhibit the growth of other bacteria, but colonies of only a certain type of microorganisms grow. What are these nutrient environments called?
Universal
Special
Selective
Synthetic
Differential-diagnostic
In the study of the drug was isolated pure culture of gram-negative bacteria. What medium should be used to study the biochemical properties of bacteria to differentiate them by enzymatic activity?
A multicolored of Gissa
Blood agar
Meat-peptone agar
Meat-peptone broth
Semi-liquid meat-peptone agar
The wound discharge material was sent to the laboratory for bacteriological examination. What nutrient media should be used to isolate aerobic bacteria?
A. Levin's environment.
Levin's environment.
The environment of Kitt-Tarotzi.
Blood agar.
Alkaline water.
MPA.
For the laboratory diagnosis of many infectious diseases use the bacteriological method. What is the purpose of Step 1 of this method?
Obtaining isolated colonies
Sowing of the test material
Microscopy of the material under study
Selection and accumulation of pure culture
Identification of the test culture
What group of organisms have annular shape and linear DNA molecules that form chromosomes of simple structure (lacking histones)?
Viruses
Fungi
Bacteriophages
Bacteria
Protozoa
Bacteria are unicellular organisms capable of being autonomous. What bacterial structures play a major role in protein synthesis?
Mesosomes
Cytoplasmic membrane
Ribosomes
Cytoplasm
Cytoplasmic inclusions
The structure of the bacterial cell, which provides increased resistance of the microbes to the effects of environmental factors and has the ability to persist for a long time, can be detected by staining smears by the method of Ozhek. What is it called?
Capsule
capsule
Flagella
Plasmid
Pili
In the course of microbiology, students were introduced to the microscopic method of diagnosis. What are the properties of bacteria studied by this method?
Morphological, tinctorial
Cultural
Antigenic
Toxigenic
Biochemical
Different staining methods are used to study the morphological features of microorganisms. For what purpose do they use simple methods?
To color the capsules
To study the shape and size of microorganisms
To identify capsules
To differentiate bacteria into gram-positive and gram-negative ones
To color flagella
The process in which DNA isolated by the lysis of one bacterium penetrating another bacterium leads to a change in its phenotype is called:
Conjugation.
By sex.
Transduction.
Transformation.
Transfection.
In biotechnological processes for the production of therapeutic and prophylactic drugs the procedure of introducing human genes into the bacterial genome by means of bacteriophage is used. What is this process called?
Transduction.
Transformation.
Broadcast.
Conjugation.
Deletion.
Bacteria may also contain chromosomal inherited elements - plasmids. The presence of plasmid genes may be manifested by:
Resistance to dyes
Multiple drug resistance
Resistance to physical factors
Spore-forming ability
Mobility
During transduction, DNA is transferred from one cell to another by bacteriophages. There are three types of transduction:
nonspecific, specific, abortive;
nonspecific, integral, abortive;
specific, abortive, virulent;
virulent, nonspecific, specific;
replication, mapping, integration.
The issue of antibiotic prescribing is discussed at a medical convention in a patient with a septic condition. What property of the causative agent should be considered when administering the antibiotic to obtain antibiotic sensitivity data?
The presence of aggression factors
Capsule-forming ability.
Acid resistance.
Gram staining.
Spore-forming ability.
The patient has prescribed a course of etiotropic antibiotic therapy. What drugs can prevent dysbiosis when using antibiotics?
Vitamins.
Eubiotics.
Immunomodulators.
Hormonal drugs.
Desensitizing drugs.
An important characteristic of the efficacy and safety of chemotherapeutic drugs is the chemotherapeutic index (Ehrlich index), which is a ratio of two doses. Whose?
Maximum tolerable and minimal therapeutic
Minimum tolerated and maximal therapeutic
Maximum tolerated and maximal therapeutic
Minimum tolerable and minimal therapeutic
Maximum intolerable and minimal therapeutic
Specify the structure of the bacterial cell that controls the virulent properties of many species:
mesosomes;
mitochondria;
plasmid pathogenicity;
methochondria;
flagella.
Non-specific factors protecting the oral cavity from penetration of pathogenic microorganisms play an important role in the overall system of physiological stability of the organism. Which of the following components in the oral cavity is the most important factor in nonspecific protection?
Complement
Lysocim
Properdin
By Lysine
Phagocytosis
Patient has been diagnosed with acute respiratory infections. During an examination, the symptoms revealed by the doctor are characteristic of this disease. It is established that the patient secretes the pathogen into the external environment. Immunoglobulins of the class M. have been found in serum. What period of the infectious process in this case?
Microorganism.
Prodromal period.
Incubation period.
Convalescence.
Acute period
The patient was diagnosed with acute respiratory infections. During the examination, the symptoms revealed by the doctor revealed that are characteristic of this disease. It is established that the patient secretes the pathogen into the external environment. This picture corresponds to the acute period of the disease. Which class of immunoglobulins is likely to be present in the patient's blood?
Ig M.
IgG.
Ig A.
Ig E.
Ig D.
Serologic diagnosis of infectious diseases is based on the specific interaction of antibodies with antigens. What is the reaction of precipitation from a solution of antigen under the influence on it of immune serum and electrolyte?
Reaction of hemadsorption
Agglutination reaction
Complement rejection reaction
Reaction of precipitation
Neutralization reaction
For the purpose of serological diagnosis of an infection, a physician should perform a reaction of indirect (passive) hemagglutination. What should be used to make this reaction other than serum sick?
Anatoxin
Interferon
Hemolytic serum
Diagnostic serum
Erythrocyte Diagnostic
Serologic diagnosis of infectious diseases is based on the specific interaction of antibodies with antigens. What is the name of the serological reaction, based on the interaction of homologous antibodies and soluble antigens in the agar medium?
Complication of the complement
Agglutination reaction
A reaction of precipitation in gel
A reaction of ring prescription
Neutralization reaction
In the immune response of the body, a significant place belongs to the production of antibodies. Choose from the given, the interaction of which cells are needed for their synthesis:
Macrophages, T-suppressors, B-lymphocytes
Antigen-expressing cells, T-helper cells, B-lymphocytes.
Fat- cells, T-helper cells, B-lymphocytes
Macrophages, T-killers, B-lymphocytes
Langerhans cells, T-killers, B-lymphocytes
In the local immunity of the oral cavity, an important role is played by various cells of the mucous membrane and antimicrobial substances that are synthesized by them. Indicate which of the following factors are crucial in providing local immunity:
Eosinophils
B-lymphocytes
IgG
macrophages
IgA
