Bal31 removes nucleotides from both strands of DNA

E. coli exonuclease III removes nucleotides from both strands of DNA

Main repairing enzyme is

Klenow fragment is obtained from

which activity is not present in klenow fragment

Klenow fragment replaces existing nucleotides

Alkaline Phosphatase

Recircularization of vector is prevented by

Antogonisitc enzyme of alkaline phosphatase is

The frequency of 4-base cutter would be less in comparision to 6-base cutter

Partial restriction digestion can be performed by

Linkers are

Adaptors are

The problom with adaptor is that - restriction digestion may cleave the DNA insert

Blunt ended ligation is performed using

Identification of recombinant clones is a two-step procedure in

IPTG is chemical analogue of

X-gal is chemical analogue of

bacteria transformed by pUC18 vectors will be

nonrecombinant clones of pUC vectors would be

which vector allows in vitro transcription of cloned DNA

M13 can infact only

true lytic cycle is absent in

which is more efficient process for transferring viral DNA into bacterial host ?

Which gene was inserted in M13 genome for converting in a cloning vector ?

Phagemid is a hybrid of

Maximum cloning capacity of M13-based vector is

Maximum cloning capacity of phagemid vector is

Lysogeny produces

5'ends of adaptors are dephosphorylated to allow formation of adaptor-adaptor dimer

The gene that is insertionally inactivated in pUC vector

Helper phages are required for transduction in

instead of plaque, colonies are formed in

in vitro packaging can be used in

problom(s) in using natural phage lembda as cloning vector is/are

Cloning capacity of lembda insertion vector is

Cloning capacity of lembda replacment vector is

Nonessential Segment (NS) in lembda genome contain genes for

recominant lembda prodcuces

vector ZAPII is based on

which one is a replacement vector

lysis repressor gene is utilised in

pJB8 is a

pJB8 has

cosmid can utilize

essential components of a chromsome are

in YAC, CEN and ori are joined with

SUP4 gene encode

Recombinant YAC produce colonies of which color

DNA insert in YAC is cloned within

Selectable marker in YEp13 is

Which vector can not maintain as free plasmid in yeast

Yeast chromosmal replication origin is utlized in

2 mili-micro plasmid origin replication is present in

BAC contain origin of replication from

Replication control in F plasmid is relaxed

Selectable marker in BAC is

Signal required for translation of cloned gene in expression vector is

signal peptide at N-terminal of an amino acid sequences make it