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WorksheetsBio Chem Lab: M3 Enzymes
Total questions: 40
Worksheet time: 21mins
special protein molecules designed to catalyze or speed up the rate of a biochemical reaction.
Enzymes
Amylase
Protein
Amino Acids
Enzymes molecules ccelerate specific reactions regulated by?
Competitive
Non Competitive
UnCompetitive
form of regulation where the regulatory molecule (an activator or inhibitor) binds to an enzyme someplace other
Allosteric Regulation
Competitive Inhibitors
Non Competitive Inhibitors
Uncompetitive Inhibitors
Breaking down of complex molecules are one of the primary biological functions of multiple enzymes through a series of
Pathway
Consecutive Reaction
Substrate
Products
Molecules called as
Products
Substrate
Consecutive Reaction
Inhibitors
what will formed when enzymes bind to its specific substrate?
Products
Activator
Inhibitors
Pathway
Generally responsible for numerous vital reaction within the body?
Enzymes
Amino Acids
Molecules
Proteins
Most of the enzymes are?
Proteins
Amino Acids
Coenzyme
Catalase
These molecules require an additional chemical components knows as?
Cofactor
Organic
Metallorganic molecule.
Presence Inhibitor
Cofactor compose of these Elements
Fe +2, Zn +2
Mg +2, Mn +2
Na +2, Ti +2
B + 2, O +2
Cofactor, Organic, Metalloorganic molecules is known as?
Coenzymes
Enzymes Kinetics
Catalase
Catalytically Active Enzymes
catalytically active enzyme bounded with its coenzyme is known as
Holoenzyme
Coenzyme
Apoenzyme
Cofactor
catalytically active enzyme bounded with its coenzyme and with the protein part.
Apoenzyme
Coenzyme
Holoenzyme
Amylase
Increased temperature speeds up collisions between enzyme and substrate.
enzyme
substrate.
ihibitor
molecule
What will happen when you heat the enzyme in high temeperature?
Denature
Produce product
pH Directly influences
Oxidation
Increased substrate concentration provides faster interaction with enzyme until it reaches its saturation point.
Substrate concentration
Presence of inhibitors
Increasing The Temperature
Some compounds may directly or indirectly affect enzyme-substrate reaction.
Presence of inhibitors.
Substrate concentration
Increase Temperature
bind to the active site of the enzyme thereby preventing the substrate to bind.
Competitive Inhibitor
Uncompetitive Inhibitor
Noncompetitive Inhibitor
inhibitors bind reversibly to the enzyme-substrate complex.
Competitive inhibitors
Uncompetitive inhibitors
Noncompetitive inhibitors
inhibitors bind to other sites in the enzyme reducing its effectivity.
Uncompetitive inhibitors
Competitive inhibitors
Noncompetitive inhibitors
is an enzyme known to catalyze hydrogen peroxide to oxygen.
Catalase
H202
Amylase
glycosidic bonds
Biologically, this enzyme is vital in protecting the cell from the damages caused by reactive oxygen species (ROS) or the free radicals.
Catalase
Amylase
Inhibitors
Catalyze Polysaccharides
is an enzyme known to catalyze polysaccharides into simpler sugar molecules by hydrolyzing the glycosidic bonds present in polysaccharides.
Amylase
Catalase
Inhibitors
Coenzyme
This enzyme is predominantly present in human saliva and is also secreted by the pancreas into the small intestines for continued breakdown of complex sugar molecules.
Amylase
Catalase
Holoenzyme
Inhibitors
Highest Froth?
Neutral pH
Acidic pH
Alkaline pH
Lowest Froth
Acidic
Alkaline
Neutral
Lower Froth
Acidic
Alkaline
Neutral
What is the The optimum pH is?
7
3
9
6
Effect of pH:
Catalase and Hydrogen Peroxide produce?
Foam
Fizz
Blue
Yellowy Red
High Temperature
No Fizzing
Fizzing
Blue
Foam
Low Temperature
Fizzing
No Fizzing
Foam
Yellowy Red
Effect of Temperature:
High Temperature cost no fizzing due to?
Denaturing
Reacting of enzymes and hydrogen peroxide
Starch solution reacts with iodine to give a deep blue-black complex.
enzyme known to catalyze polysaccharides into simpler sugar molecules
Effect of Temperature:
Low Temperature cost fizzing due to
reaction of catalase enzymes and hydrogen peroxide
Denature
no starch
means it has starch in there.
Starch solution
Result after heating + iodine solution
Blueish
Yellowy Red
Clear
Yellowy Brown
Starch Solution
Result after heating + iodine solution and after adding Benedict solution and heating
Blueish
Yellowyred
Denature
Clear
that means it has starch in there.
Starch Solution
Starch + Saliva
High Temperature
Neutral
result is yellow brown it means no starch in there
Starch + Saliva
Starch Solution
Low Temperature
Acidic
Starch + Saliva
Result after heating + iodine solution and after adding Benedict solution and heating
Yellowy Red
Yellowy Brown
Bluish
Clear
Starch + Saliva Result after heating + iodine solution
Yellowy Brown
Yellowy Red
Bluish
Foam
indicates that theres is a reducing sugar in there and that is maltose that's the result of the amylase acting on the starch
Yellowy Red of Iodine, and Benedict Solution
Yellow Brown of Iodine
Blueish of iodine Solution
Blueish of Iodine Solution and Benedict Solution
