Font size
WorksheetsIsolation of PBMC
Total questions: 30
Worksheet time: 17mins
What is plasma?
A fluid in blood with clotting factors
A fluid in blood without clotting factors
A fluid with all formed elements
A fluid without RBCs
Which is most abundant protein?
Fibrinogen
Albumin
Globulin
Thrombin
Which is not the types of granulocytes in WBCs?
Basophils
Eosinophils
Neutrophils
Lymphocytes
What cells are PBMC
Lymphocytes
Monocytes
Macrophages
All of the above
What are the types of lymphocyes in blood?
B-Lymphocytes
T-Lymphocytes
NK Cells
Monocytes
In kidney macrophages are called as
(a)
Which type of centrifugation techniques used to isolated the cells
Differential
Density Gradient
Isopycnic
No need to centrifuge
What is added to prevent coagulation in blood?
(a)
What is the sequence of layers after centrifugation to isolate PBMC?
plasma, PBMC, eryhtrocytes, ficol
plasma, PBMC, ficol, erythrocytes
plasma, ficol, erythrocytes, PBMC
ficol, erythrocytes, PBMC, plasma
What is the volume of one square?
1 mm3
0.1 mm3
10 mm3
1 cm3
Which is the enzyme used as a marker for lysosomes
Pyruvate dehydrogenase
Phospholipase
Acid phosphatase
Succinate dehydrogenase
Liposomal fractions are isolated by
Electrophoresis
Ultracentrifugation
both a and b
Sucrose density gradient centrifugation
This is not a function of mitochondria
fatty acid breakdown
non-shivering thermogenesis
electron transport chain and associated ATP production
glycolysis and associated ATP production
How you can measure the absorbance in LDH assay
measure concentration of Lactate
Measure concentration of pyruvate
Measure concentration of NAD+
Measure concentration of NADH+H+
Which of the following is marker of cytosol?
Succinate dehydrogenase
Lactate Dehydrogenase
Acid Phosphatease
None of the above
Which of the following is marker of mitochondria (more than one answer)
PDH
LDH
SDH
All of the above
This cell organelle participates actively in animal apoptosis
nucleus
vacuoles
mitochondria
chloroplast
This cannot be killed by apoptosis
immune cells
cells with DNA damage
cancer cells
a cell infected with viruses
Caspases are involved in
Cell Division
Necrosis
Apoptosis
Inflammation
The characteristic feature of apoptosis under light microscopy
Cellular Swelling
Nuclear Compaction
Intact cell membrane
all of the above
This is cancerous state of blood
Uremia
Chloremia
Leukemia
Proteinemia
A patient is suspicious of having breast cancer. What type of test will a physician conduct to diagnose the cancer
blood test
mammography
CT scan
pap test
Carcinogens and produce malignant tumor
Carcinogens and produce malignant tumor
carcinogens and produce non-malignant tumor
mutagenic agents and do not produce malignant tumor
mutagenic agents and produce benign tumor
The nucleus of cancerous cells becomes
Unchanged
Degenerated
Abnormally large
Hypertrophied
Cancerous cells are more easily damaged by radiation than normal cells as they
differ in structure
undergo rapid division
are nutrition-starved
none of these
Glycerol is added to DNA samples before they are loaded to the wells of gel. The function of glycerol is to
Stabilize DNA structure
Provide density to the DNA
Provide charge to the sample
None of the above
Electrophoresis is used to
Separate DNA fragments
Identify DNA fragments
Purify DNA fragments
Both A and C
What is Ethidium Bromide
Buffer
Dye
DNA solution
Restriction Enzyme
What is the use of DCPIP
It blocks the ETC
It provides alternate electron acceptor
It preserves protein structure
It prevents growth of microorganism
What is the use of Sodium Azide
It blocks the ETC
It provides alternate electron acceptor
It preserves protein structure
It prevents growth of microorganism
