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gram stain

Total questions: 16

Worksheet time: 14mins

Name
Class
Date
1.

Gram stain is a

a)

Simple stain

b)

Differential stain

c)

Capsular stain

d)

Acidfast stain

2.

primary stain of gram stain is

a)

Crystal violet

b)

Safranin

c)

Acetone

d)

Carbol fuchsin

3.

All are reagents of gram stain Procedure except

a)

Methylviolet

b)

Acetone

c)

Methylene blue

d)

Carbol fuchsin

4.

Step 1:

Smear stained with

a)

Safranin

b)

Crystal violet

c)

Acetone

d)

Gram's iodine

5.

After step 1,how does gram positive and gram negative bacteria appear?

a)
b)
c)
d)

none of the above

6.

Identify the step 2:

addition of

a)

Crystal violet

b)

Safranin

c)

Iodine

d)

Acetone

7.

After step 2,how does gram positive and gram negative bacteria appear?

a)
b)
c)
8.

Function of mordant is

a)

Dissolve the dye-iodine

b)

Dissociate the stain from cytoplasm

c)

Form big dye-iodine complex

d)

All of the above

9.

After step 3,how does gram positive and gram negative bacteria appear?

a)
b)
c)
10.

Crucial step in Gram stain is addition of

a)

Crystal violet

b)

Iodine

c)

Acetone

d)

Safranin

11.

duration of alcohol decolourizer is

(a)  

12.

After step 4,how does gram positive and gram negative bacteria appear?

a)
b)
c)
13.

According to pH theory, gram positive bacteria are

a)

Neutral

b)

Acidic

c)

More Acidic

d)

More basic

14.

This is a cellwall of

a)

gram negative

b)

gram variable

c)

gram positive

d)

all of the above

15.

Choose the uses of gram stain(Multiple answers)

a)

To start empirical treatment

b)

Can stain fungi such as Candida.

c)

Differentiate acid fast from non acid fast

d)

To classify virus into gram positive and gram negative

16.

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