wayground logo

Free Printable Worksheets

Font size

S
M
L
XL
Worksheets

Biotechnology

Total questions: 100

Worksheet time: 1hrs 9mins

Name
Class
Date
1.

What is a cDNA?

a)

collection of total genomic DNA

b)

non-coding RNA molecule (containing about 22 nucleotides) found in plants,

c)

complementary DNA molecules synthesized from mRNA

d)

small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules

2.
Human insulin produced by genetic engineering is better because (a) it acts faster (b) it lasts longer in the body (c) it does not cause allergic reactions
a)
a,b, and c
b)
a and c
c)
b and c
d)
a only
3.
The structure from which insulin mRNA can be obtained is ………………………………
a)
pancreas
b)
islets of Langerhans
c)
blood plasma
d)
liver
4.
in producing insulin by genetic engineering, what is the first step to take?
a)
isolate insulin from pancrease
b)
isolate insulin mRNA from pancreas
c)
isolate insulin DNA from pancreas
d)
isolate insulin from animal tissue
5.

This is the practice of inserting the DNA of another species into an organism's DNA so that the organism gains a new trait.

a)

Cloning

b)

Gel Electrophoresis

c)

Genetic Engineering

d)

DNA Fingerprinting

6.

Restriction enzymes are analogous to what common school supply?

a)

glue

b)

scissors

c)

paper

d)

highlighter

7.

The site where restriction enzymes cut are a called:

a)

cut sites

b)

restriction sites

c)

recognition sites

d)

cleave sites

8.

Where are restriction enzymes naturally found?

a)

in humans

b)

in bacteria

c)

in algae

d)

they are not natural - all restriction enzymes are made in the lab

9.

If a linear piece of DNA contains 3 recognition sites for a restriction enzyme. How many pieces will be produced when the restriction enzyme cuts the DNA?

a)

2

b)

3

c)

4

d)

5

e)

6

10.

What enzyme binds to the promotor of the plasmid to transcribe the gene inserted into the plasmid?

a)

DNA polymerase

b)

helicase

c)

RNA polymerase

d)

ligase

11.

What enzyme is used to ensure that the recombinant DNA created is bonded together along the sugar-phosphate backbone?

a)

DNA polymerase

b)

RNA polymerase

c)

ligase

d)

helicase

12.

Biological cloning is frequently used to make which of the following:

a)

Human growth hormone

b)

Human insulin

c)

genetically modified oil eating microbes

d)

pest resistant crops

e)

all of the options

13.

Why are primers needed for the PCR process?

a)

they are what makes copy of the DNA

b)

they are used to build more copies of the DNA

c)

they attach to sites on the DNA on either side of the DNA to be copied

d)

they are what change temperature to copy the DNA

14.
A cell is made up almost entirely of _______________. 
a)
carbohydrates
b)
proteins
c)
fats
d)
nucleic acids
15.
A protein of interest is _____________. 
a)
the entire genome of the organism
b)
a section of DNA (a gene) that codes for a protein
c)
a protein that can be used to treat a disease
d)
a protein that has an interesting shape
16.
Which of the following is a circular DNA from bacteria that can hold a foreign gene?
a)
plasmid
b)
restriction enzyme
c)
ligase
d)
protein expression
17.
Which of the following is an enzymatic protein that acts as DNA glue?
a)
plasmid
b)
restriction enzyme
c)
ligase
d)
protein expression
18.
Which is true about plasmids?
a)
Plasmids can be cut at specific sequences called restriction sites.
b)
Plasmids contain a promoter sequence that defines where transcription begins.
c)
Plasmids can contain an antibiotic resistance gene.
d)
All of the statements are true about plasmids.
19.
Which of the following model organisms is often used in rDNA technology to make insulin?
a)
small squid
b)
mouse
c)
fruit fly
d)
E. coli
20.

A technique for making millions of copies of a specific region of DNA.

a)

DNA ligase

b)

restriction enzymes

c)

gel electrophoresis

d)

polymerase chain reaction

21.

From where does Taq polymerase come?

a)

Bacteria that live in hydrothermal vents/hot springs.

b)

Cambodia

c)

The rain forest

d)

The CDC

22.

At what temperature does the Denature step of PCR occur?

a)

95° C

b)

50° C

c)

72° C

d)

Any temperature

23.

At what temperature does the Anneal step of PCR occur?

a)

95° C

b)

50° C

c)

72° C

d)

Any temperature

24.

At what temperature does the Extension step of PCR occur?

a)

95°C

b)

50-65°C

c)

72°C

d)

Any temperature

25.
What happens during the Anneal step of PCR?
a)
Primers are created.
b)
The primers attach to the target DNA region.
c)
Primers copy the new DNA strand.
d)
Primers sequence DNA
26.
What is the function of a primer?
a)
To identify the particular region of DNA to be copied by PCR.
b)
To copy DNA.
c)
To create DNA nucleotides.
d)
To maintain the temperature of the PCR reaction.
27.
What is the purpose of the Extension step of PCR?
a)
To create the primers.
b)
To extend the time it takes to produce DNA.
c)
To allow polymerase to create the complementary strands of DNA.
d)
To allow substrates to copy DNA sequences.
28.
How many cycles of PCR does it take to produce a double-stranded DNA fragment of the desired length?
a)
1
b)
2
c)
3
d)
4
29.
What type of bonds hold the two strands of DNA together?
a)
Hydrogen bonds
b)
Covalent bonds
c)
Dipole-Dipole bonds
d)
Ionic bonds
30.
PCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?
a)
1
b)
10
c)
30
d)
Over 1 billion
31.

If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?

a)

2

b)

4

c)

8

d)

32

32.

What is gene therapy?

a)

Introduce a functional gene into a person who lacks this

gene

b)

Treating recessive

diseases

c)

Both

33.

Long term expression, infects neuroglia

a)

Retrovirus

b)

Adenovirus

c)

Herpes virus

d)

Adeno-associated virus

34.

Methods for chemical transfection:

i) Calcium chloride

ii) DEA dextran

iii) Cationic lipid

iv) Anionic lipid

a)

All above

b)

i, ii, iii

c)

ii, iii, iv

d)

iii, iv

35.

It is the transfer of genetic material for the purpose of treating human disease.

a)

chemotherapy

b)

gene therapy

c)

gene transfer

d)

gene editing

36.

A disease caused by mutation in the genetic material or DNA

a)

Cloning Disease

b)

Mutant disease

c)

Genetic disease

d)

All of the Above

37.

A/an "_____" gene is introduced into the patient to replace an "_____" disease causing gene.

a)

normal - abnormal

b)

good - bad

c)

abnormal - normal

d)

bad - good

38.
 how can  one fix  a genetic disorder by gene therapy 
a)
You can take out or change a section on a chromosome 
b)
split the chromosomes 
c)
add enzymes 
d)
add buffer 
39.

The first reported case of gene therapy in 1999 by ________

a)

Jesse Gelsinger

b)

Albert Einstein

c)

Robert Hook

d)

Dr. Victoria Ashton

40.

Which of the following best describes a gene? 

a)

A section of DNA

b)

Circular DNA found in bacteria

c)

DNA that has genes from different organisms

d)

A bundle of DNA

41.

Which of the following best describes transgenic organism? 

a)

Changing bacterial DNA by adding genes to it

b)

Any organism that has DNA from a different species

c)

Any organism that has DNA from the same species

d)

A tool used by scientists to separate chunks of DNA

42.

What is bioremediation, and how has genetic engineering helped with this process?

a)

Bioremediation is using living things to clean up pollution. Scientists have created GMO bacteria that eat oil.

b)

Bioremediation is using living things to clean up dirt and debris. Scientists have created GMO bacteria that eat mud.

c)

Bioremediation is using living things to destroy a harmful insect population. Scientists have created GMO bacteria that kill insects.

d)

Bioremediation is using living things to control the climate. Scientists have created GMO bacteria that better withstand extreme temperatures.

43.

Using genetically modified pigs to harvest heart valves for humans demonstrates which benefit of genetically modified organisms?

a)

animal models

b)

livestock improvement

c)

tissue and organ donors

d)

pharmaceutical production

44.

When researchers apply for a patent from the U.S. Patent and Trademark Office, for what process are they seeking approval?

a)

creating a transgenic animal

b)

reproductive cloning

c)

therapeutic cloning

d)

nanotechnology

45.

When scientists are able to use an organ from another species for transplantation, this is known as:

a)

a promotor.

b)

pharming.

c)

a xenograft.

d)

a totipotent.

46.

What is the biggest challenge to the successful use of pig organs in human transplants?

a)

The organs are too small

b)

Rejection

c)

Protest by animal rights groups

d)

Expense

47.
The unpaired nucleotides produced by the action of restriction enzymes are referred to as
a)
sticky end.
b)
base sequence.
c)
single strands.
d)
restriction fragments.
48.
In order to insert a human gene into plasmid, both must 
a)
code for the same gene product.
b)
be cut by the same restriction enzyme.
c)
originate from the same type of cell.
d)
have identical sequences.
49.
One of the goals of the Human Genome Project is to
a)
increase the number of genes in the human genome
b)
map the location of only the most important genes on each chromosome
c)
clone the entire human genome in bacteria
d)
determine the nucleotide sequence of the entire human genome
50.
Which technique would most likely be used by forensic scientist?
a)
Gene cloning
b)
Gene therapy 
c)
DNA fingerprinting 
d)
Karyotyping 
51.
Which best describes the impact that the Human Genome Project has has on society?
a)
It has helped scientists determine that certain genes can cause diseases 
b)
It has led to discrimination against genetically flawed individuals
c)
It has led to the appearance of new diseases
d)
It has cost society billions of dollars without any major benefits
52.

) In plant tissue culture technique surface sterilization of explant is made by…………

a)

Hydrochloric acid

b)

Sulphuric acid

c)

Mercury chloride

d)

Nitric acid

53.

The two strand of double helix model of DNA are holdind together by……..

a)

Sugar & nitrogen base

b)

Sugar &phosphate group

c)

Phosphate &nitrogen base

d)

Phosphate &phosphate group

54.

In DNA replication okazaki fragments on lagging are joined together by enzyme

a)

Helicase

b)

Primase

c)

DNA ligase

d)

RNA ligase

55.

Topoisomerase is involved in….. ………………

a)

Producing RNA Primer

b)

Secretion of DNA strand

c)

Producing Nick in DNA

d)

T-RNA formation

56.

Restriction Endonucleases are ……………enzyme

a)

Bacterial

b)

Viral

c)

Fungal

d)

Algal

57.

Agrobacterium cell consist of ………..as independent replication circular DNA

a)

Cosmid

b)

Plasmid

c)

Ti-Plasmid

d)

L-Plasmid

58.

……………Medium is commonly used in tissue culture technique

a)

PDA

b)

M.S.

c)

Agar-Agar

d)

Czapek Dox

59.

The term ‘Totipotency’ was given by _____________.

a)

T.H. Morgan

b)

Haberlandt

c)

Schwann

d)

De Robertis

60.

In PCR reaction .....enyme is used.

a)

Taq polymerase

b)

Reductase

c)

Phosphatse

d)

Dehydrogenase

61.

The grafting in which stock and scion have different diameter is

a)

Splice approach grafting

b)

Tongue grafting

c)

Cleft grating

d)

None of the above

62.

The piece of tissue or organ excised from a plant for culturing in a suitable medium is

a)

Plantlets

b)

Callus

c)

Explant

d)

Embryoids

63.

The ability of an isolated mature plant cell to dedifferentiate to a meristematic state and then to redifferentiate to a whole plant is

a)

Totipotency

b)

The principle applied in tissue culture

c)

Both a & b

d)

None of the above

64.

somatic embrogenesis and organohenesis are

a)

Regeneration methods in tissue culture

b)

Methods of callus formation

c)

Dedifferentiation method

d)

All the above

65.

The embryos formed from somatic cells are

a)

Somatic buds

b)

embryoids

c)

zygote

d)

None of the above

66.

The capacity to generate a whole plant from any cell/explant

a)

micropropagation

b)

Tissue culture

c)

Totipotency

d)

somatic hybridisation

67.

The method of producing thousands of plants through tissue culture is called

a)

somatic hybridiastion

b)

totipotency

c)

micro-propagation

68.

which of these plants are examples of plants grown using tissue culture?

a)

banana/plantain

b)

lemon

c)

mango

d)

apple

e)

china rose

69.

Put it in order:

a)

callus - explant - plantlet

b)

plantlet - callus - explant

c)

explant - callus - plantlet

70.
The study of the genome is known as
a)
Biology
b)
Genomics
c)
Genes
d)
Heredity
71.
The genomes of two unrelated humans are ___% the same.
a)
90.0
b)
0.1%
c)
78%
d)
99.9%
72.
How many base pairs (bp) are in the human genome?
a)
1 billion bp
b)
4 billion bp
c)
3 billion bp
d)
3.5 billion bp
73.

Haploid content of human DNA has _________base pairs

a)

4.6 x 106

b)

48502

c)

3.3 x 10 9

74.

The area in Biology that uses high speed computational devices for storing enormous amount of data, retrieval and analysis is called____

a)

Bioinformatics

b)

Biotechnology

c)

Biostatistics

75.

Many non human model organisms have also been sequenced through Human Genome project. The nematode among the following is_____

a)

Drosophila

b)

Arabidopsis

c)

Caenorhabditis elegans

76.

The DNA fragments in Human Genome Project were sequenced using automated DNA sequencers that was based on the principle developed by ______

a)

Fredrick Grifith

b)

Watson and Crick

c)

Frederick Sanger

d)

Har Gobind Khorana

77.

Which chromosome in Human Genome has the fewest genes?

a)

Chromosome X

b)

Chromosome Y

c)

Autosome 13

d)

Chromosome 1

78.

Largest known human gene is ______at 2.4 million bases

a)

Desktrophin

b)

Disctropin

c)

Dystrophin

79.

________ contains all the genetic information needed for an organism to grow and survive

a)

gene

b)

genome

c)

proteome

d)

haplotype

80.

What are the variations in specific nucleotides that are linked to human disorders called?

a)

proteomes

b)

haplotypes

c)

SNPs

d)

genome

81.

Genetic variation that are located close together are called

a)

haplotypes

b)

proteomes

c)

HapMap

d)

SNPs

82.

The field of _____uses computers to organize information created by sequencing the human genome

a)

Bioinformatics

b)

genomics

c)

SNPs

d)

gel electrophoresis

83.
Any change in the sequence of DNA is...
a)
transgenic shift
b)
Single Genotype
c)
Monohybrid Trait
d)
Mutation
84.
What causes mutations?
a)
Mistakes in replication or environmental substances
b)
The amino acids
c)
Passed down by genetics
d)
It's how your born
85.
Cause mutations in DNA
a)
Endocrine Disruptors
b)
Chemical Mutagen
c)
Neurotoxins
d)
Carcinogen
86.
Cause cancer
a)
Chemical Mutagen
b)
Carcinogens
c)
Allergens
d)
Teratogens
87.
Cause birth defects
a)
Carcinogen
b)
Teratogens
c)
Endocrine Disruptors
d)
Allergens
88.
What are things that cause changes in the DNA sequence called?
a)
dangerous
b)
unfortunate
c)
interferon
d)
mutagens
89.
Example of mutagens
a)
all
b)
x-rays
c)
ultraviolet radiation
d)
tobacco
90.

Which is an example of a chemical mutagen?

a)

sugar

b)

chocolate

c)

insulin

d)

alcohol

91.

In order for offspring to get a genetic mutation, the mutation must occur in what type of cell?

a)

Cells with no nucleus.

b)

Any body cell after birth.

c)

Cells with no cell wall or cell membrane.

d)

Cells like eggs or sperm cells that are undergoing reproduction.

92.
ATTTGAGCC- Original
ATTGAGCC - Mutated
The example above is an example of a 
a)
Insertion- Frameshift
b)
Deletion- Substitution
c)
Deletion -Frameshift
d)
All of the above
93.
What mutation has occurred here? 
T-G-A-C-C-A
T-G-A-G-C-A
a)
Substitution
b)
Deletion
c)
Insertion
d)
Frameshift 
94.
Identify the SUBSTITUTION mutation form the following: 
ATG CCA AAT
a)
ATG TCA AAT
b)
ATG CCT AAA T
c)
ATC CA  AT
d)
ATG CCA AAT
95.
Identify the DELETION mutation form the following: 
ATG CCA AAT
a)
ATG TCA AAT
b)
ATG CCT AAA T
c)
ATC CA  AT
d)
ATG CCA AAT
96.
Identify the ADDITION mutation form the following: 
ATG CCA AAT
a)
ATG TCA AAT
b)
ATG CCT AAA T
c)
ATC CA  AT
d)
ATG CCA AAT
97.

Where do we insert vectors?

a)

bacterial host cells

b)

restriction enzymes

c)

culture media

d)

a and b only

98.

Below are correct statements regarding a probe EXCEPT

a)

A probe is used to search for its complementary sequence in a sample genome.

b)

The probe is labeled with a radioactive or chemical tag that allows its binding to be visualized

c)

A probe is a single-stranded sequence of DNA or RNA

d)

A probe is a double-stranded sequence of DNA or RNA

99.

A genomic library can be built in a

a)

phage library form

b)

plasmid library form

c)

YACs library form

d)

All of above

100.

Below are components/enzymes used in cDNA library construction EXCEPT

a)

using isolated cellular mRNA

b)

using genomic DNA

c)

using the cell's transriptome

d)

using reverse transcriptase

Similar Resources on Wayground

Blood and Cardiovascular

98 questions

Blood and Cardiovascular

quiz

Worksheet

•

12th Grade - University

Test 1 Zoology Ecosystem, Fish classification and Vocabulary

100 questions

Test 1 Zoology Ecosystem, Fish classification and Vocabulary

quiz

Worksheet

•

University

Biol111 Practice Exam 4

100 questions

Biol111 Practice Exam 4

quiz

Worksheet

•

University

ICT-jestkihentai

100 questions

ICT-jestkihentai

quiz

Worksheet

•

University

A. P Biology Cells and Membrane

101 questions

A. P Biology Cells and Membrane

quiz

Worksheet

•

12th Grade - University

Lab Practical 1 REVIEW

99 questions

Lab Practical 1 REVIEW

quiz

Worksheet

•

University

Life Science/Biology FINAL Test

100 questions

Life Science/Biology FINAL Test

quiz

Worksheet

•

7th Grade - University

BIOCHEM PRELIM

100 questions

BIOCHEM PRELIM

quiz

Worksheet

•

University