WorksheetsCLIN286 Review
Total questions: 55
Worksheet time: 28mins
A clear red colony with a black center on the XLD agar is most likely:
E.coli
Shigella
Salmonella
Yersinia
Biological safety cabinets use this type of filter to remove aerosols?
bag filters
fiberglass filters
HEPA filters
Electrostatic filters
Eye wash stations should be able to provide continuous water flow for how long?
10 minutes
60 minutes
45 minutes
15 minutes
Why are vancomycin, colistin and nystatin added to culture media?
To enhannce growth of N.gonorrhoeae
To provide a capnophilic environment
To inhibit normal genital flora
To increase sensitivity of the oxidase test
Which of the following may be used to differentiate Salmonella species from Shigella species?
Colony morphology on Blood agar
Microscopic morphology in Gram stain
Presence of "H" antigens
Glucose fermentation
Antimicrobial resistance resulting from normal genetic, or physiologic state of a microorganism is ________ resistance.
intrinsic
inherent
micro-organism mediated
clinical
HUS is a condition that can be caused by what organism?
Shigella sp.
E.coli 0157:H7
Campylobacter sp.
Plesiomonas sp.
The organism most commonly associated with subacute bacterial endocarditis is?
Staphylococcus aureus
Streptococcus pneumoniae
B-hemolytic streptococcus
A-hemolytic streptococcus
Beta-lactams would not be effective in treating which of the following?
Streptococcus pyogenes
Mycoplasma pneumoniae
Haemophilus influenzae
Pasteurella multocida
One way to differentiate Listeria or Corynebacterium from Bacillus spp. is:
catalase reaction
motility
spore formation
hemolysis
A gram positive coccus that is catalase positive, coagulase negative, and resistant to novobiocin is:
S. aureus
S. epidermidis
S. saprophyticus
S. lugdunensis
This organism will not grow on sheep blood agar without the addition of vitamin B6:
Abiotrophia
E. faecalis
S. mutans
Kingella
Which species of Burkholderia is the one most commonly found in clinical specimens?
Burkholderia ralstonia
Burkholderia pseudomallei
Burkholderia cepacia
Burkholderia mallei
The acid fast stain is specifically designed for bacteria whose cell walls contain:
Peptidoglycan
Mycolic acid
Teichoic acids
Disaccharide pentapeptide subunits
CAMP factor is produced by?
Enterococcus faecalis
Streptococcus agalactiae
Streptococcus pneumoniae
Streptococcus pyogenes
Which gram negative anaerobe is sensitive to vancomycin?
Prevotella sp.
Bacteroides sp.
Fusobacterium sp.
Porphyromonas sp.
What is the gram stain of an organism that is nagler test positive ,and reverse camp positive?
Gram negative bacilli
Gram positive bacilli
Gram positive bacilli (large)
Gram negative coccobacilli
Nutritionally variant Streptococci require what ingredient not found in most conventional media?
Naladixic acid
NAD
egg
Vitamin B6
Bacitracin, PYR, CAMP factor, and hippurate are tests most likely performed on a(n)____________Streptococcus.
Alpha hemolytic
Beta hemolytic
nonhemolytic
gamma hemolytic
SPS susceptibility may be used to differentiate species with this gram stain:
Gram positive cocci
Gram negative coccobacilli
Gram positive bacilli
Gram negative cocci
The test that can differentiate P. mirabilis from P. vulgaris is:
PYR
Indole
Oxidase
Lysine Decarboxylase
The most common form of gonococcal infection in neonates is:
Impetigo
Urethritis
Conjunctivitis
Pharyngitis
Bacteria that grow at temperatures as high as 50-55 Celsius are known as:
Psychrophiles
Cryophil
Mesophiles
Thermophiles
A mordant that is applied after the primary stain to chemically bond the alkaline dye to the bacterial cell wall is:
Safranin
Grams decolorizer
Crystal violet
Grams iodine
One test used to differentiate S. aureus from S. lugdenensis is:
PYR
Catalase
Novobiocin
Bacitracin
What name is given to the type of hemolysis that produces complete hemolysis and a greening discoloration of the medium?
Beta
Alpha
Gamma
Kappa
Which of the following specimens requires a gram stain?
Vaginal-rectal for Grp.B Streptococcus
Skin Swab
urine
stool
Which of the following structures contains genes for enzymes and antibiotic resistance?
Plasmid
Pilus
Capsule
Plasma membrane
The most critical step in the Gram stain is the application of the:
Safranin
Crystal violet
Grams decolorizer
Grams iodine
What is the maximum length of time that un-preserved urine can be refrigerated before processing?
2 Hours
4 Hours
8 Hours
24 Hours
Which of the following microorganisms has not been linked to UTI’s?
E. coli
Pseudomonas
Klebsiella
Haemophilus
An organism described as oxidase positive, Alk/NC on TSI media, and produces a bright bluish green pigment on MH.
Ralstonia pickettii
Burkholderia mallei
Stenotrophomonas maltophilia
Pseudomonas aeruginosa
The Gram stain from a Sputum sample shows abundant PMN's and no Squamous epithelial cells how should you proceed ?
Reject
Process
re-make gram stain
re-examine slide
What ingredient must be present in the medium used to test for indole production?
Sodium thiosulfate
Tryptophan
Diacetyl
Phenylalanine
Salmonella differs from Shigella in which of the following properties?
oxidase
indole
motility
lysine decarboxylase
Which of the following media components would be considered Selective/Inhibitory ?
Crystal violet
Glucose
IsoVitalex
Vitamin K
Looking at the following picture, what two characteristics best describe this organism?
beta hemolytic, optochin susceptible
alpha hemolytic, bile solubility negative
gamma hemolytic, optochin resistant
alpha hemolytic, bile solubility positive
Which of the following media's is considered enriched?
Chocolate
Thayer-Martin (TM)
Laked Kanamycin Vancomycin (LKV)
Bile esculin
What type of Hemolysis is being displayed in the picture below?
α
γ
β
Ω
Which test is being displayed in the picture below?
Oxidase
PYR
Indole
Catalase
How would you interpret the TSI results: Alkaline slant/Acid butt?
Glucose, lactose and/ or sucrose fermented.
Glucose, lactose and/ or sucrose fermented.
Glucose only fermented.
Glucose , lactose and/or sucrose not fermented.
MacConkey agar showing bacterial growth as colorless colonies, what can you know about the bacteria growing?
Gram-positive lactose fermenter
Gram-negative non-lactose fermenter
Gram-negative lactose fermenter
Gram-positive non-lactose fermenter
Which organism is the most commonly isolated as VRE?
Enterococcus faecalis
Enterococcus gallinarium
Enterococcus faecium
Enterococcus avium
Which terms describes an organism that requires an atmosphere containing CO2 for growth?
capnophile
anaerobe
aerobe
microaerophilic
The minimum inhibitory concentration (MIC) result recorded in antimicrobial testing can be defined as the:
maximum concentration of antimicrobial that should be used .
lowest antimicrobial concentration that inhibits visible bacterial growth.
minimum concentration of antimicrobial that should be used.
highest antimicrobial concentration that inhibits visible bacterial growth.
When measuring zones of inhibition on translucent media _______ light should be used.
transmitted
UV
reflected
Fluorescent
The Breakpoint is the highest plasma concentration of the drug that can safely be achieved in the patient .
True
False
Which of the following species of Candida produces the characteristic thick walled chlamydospores
C. albicans
C. tropicalis
C. krusei
C. parakru
You receive a swab from a decubitus ulcer how do you proceed with the specimen?
Process
Reject
Centrifuge
Refrigerate
What is the antibiotic of choice for Anaerobic infections?
Penicillin
Metronidazole
Vancomycin
Linezolid
The fluorochrome stain used to study a fungus is:
Auramine rhodamine
Acridine orange
Calcofluor white
Methylene Blue
Dermatophytes are fungi which:
Produce morphologically identical macroconidia in all genra
Live in superficial keratinized areas of the body: skin, hair and nails
Require complex media for growth
Cause systemic infections
Which organism may be difficult to isolate in culture due to autolysis?
Streptococcus sanguis
Streptococcus pneumoniae
Streptococcus anginosus
Streptococcus agalactiae
Which of the following would be considered a Bioterror organism?
Neisseria meningitidis
Burkholderia cepacia
Clostridium botulinum
Francisella tularensis
Another test that could identify an organism that is resistant to Kanamycin, Vancomycin and Colistin is:
Indole
Growth on 20% bile
Catalase
reverse camp
