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CHAPTER 6 EXPRESSION OF BIOLOGICAL INFORMATION

Total questions: 50

Worksheet time: 55mins

Name
Class
Date
1.

The DNA molecule consists of monomers known as

a)

nucleotide

b)

nucleoside

c)

pyrimidine

d)

purine

2.

A new DNA strand only elongated in the 5’ to 3’ direction because

a)

DNA polymerase adds nucleotides at the 5’ end

b)

Replication must progress toward the replication fork

c)

Okazaki fragments prevent elongation in the 3’ to 5’ direction

d)

DNA polymerase adds nucleotides only to the free 3’ end

3.
The "central dogma" states that genetic information flows from 
a)
Protein-->mRNA-->DNA
b)
Protein-->DNA-->mRNA
c)
mRNA-->DNA-->Protein
d)
DNA-->mRNA-->Protein
4.
Which do DNA and RNA have in common?
a)
Both are double stranded.
b)
Both contain ribose molecules.
c)
Both contain phosphate groups.
d)
Both contain uracil.
5.

Function of primase in the figure as

a)

Unwinding the DNA double strand to allow DNA polymerase to function

b)

Forming replication fork

c)

Making short strands of RNA at the site where replication begins

d)

Hydrolyzing ATP to facilitate DNA unwinding

6.
Smaller segments of RNA nucleotides that transport amino acids to the ribosomes.
a)
mRNA
b)
tRNA
c)
rRNA
d)
DNA
7.

Which enzyme joins the Okazaki fragments in the lagging strand?

a)

Helicase

b)

DNA Polymerase

c)

Ligase

d)

Primase

8.

DNA ligase are enzymes that

a)

Unwind the double helix of DNA during replication

b)

Link the nucleotides of the new DNA strand along the template of the old strand

c)

Rejoin the two DNA strands (one new and one old) after replication

d)

Annealing the Okazaki fragments

9.

This picture is illustrating the ____ nature of DNA.

a)

antiparallel

b)

semiconservative

c)

helical

d)

radioactive

10.

What is the product of transcription and where does it go after the process?

a)

rRNA, ribosome

b)

rRNA, cytoplasm

c)

mRNA, ribosome

d)

mRNA, cytoplasm

11.

Which enzyme is responsible for adding the RNA primer to the template strand?

a)

Helicase

b)

DNA Polymerase

c)

Ligase

d)

Primase

12.

What is the name of enzyme for amino acid activation?

a)

aminoacyl tRNA synthase

b)

aminoacyl tRNA synthetase

c)

aminoacyl rRNA synthase

d)

aminoacyl rRNA synthetase

13.
The process of DNA replication is called semi conservative because it results in
a)
none of these
b)
Two DNA molecules.  One original molecule and one with two new strands
c)
two exact copies with new strands
d)
two identical molecules, each with 1 original strand and one new.
14.

The backbone of polynucleotide is made up of

a)

amino acids and sugars

b)

nucleotide bases, including thymine

c)

nucleotide bases, including uracil

d)

sugar-phosphate molecules

15.

In DNA

replication, new DNA nucleotides are added to a growing DNA by…………………

a)

DNA helicase

b)

DNA polymerase

c)

RNA polymerase

d)

RNA primer

16.

How do the leading and the lagging strands differ?

a)

The leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.

b)

The leading strand is synthesized at twice the rate of the lagging strand.

c)

The leading strand is synthesized in short fragments that are ultimately stitched together, whereas the lagging strand is synthesized continuously.

d)

The leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5? end.

17.

The following events occur in the replication of DNA.


1. Bonds between complementary bases break

2. Bonds between complementary bases form

3. DNA molecule uncoils

4. Opposite strands separate

5. Sugar-phosphate backbone form

6. Free nucleotides align with complementary nucleotides on each strand


In which order do these events take place?

a)

1 3 6 4 2 5

b)

3 1 4 6 2 5

c)

3 6 1 4 5 2

d)

4 3 1 6 5 2

18.

Which of the following is the major enzyme that involved in DNA

replication?

I. Helicase

II. Reverse transcriptase

III. Topoisomerase

IV. DNA polymerase

V. Aminoacyl-tRNA synthetase

a)

I, III and IV

b)

I, III and V

c)

I,II, III and IV

d)

II, III, IV and V

19.
Replicate the following strand of DNA: 
AATCATGGA
a)
AATCATGGA
b)
TTAGTACCT
c)
UUAGUACCU
d)
GGATAUCUA
20.

What are the three steps in elongation stage of translation (in sequence order)?

a)

Codon recognition, translocation, peptide bond formation

b)

Codon recognition, peptide bond formation, translocation

c)

Peptide bond formation, translocation, codon recognition

d)

Peptide bond formation, codon recognition, translocation

21.

Transcription occur in

a)

Cytoplasm

b)

Ribosome

c)

Nucleus

22.

What happen when it reach stop codon during termination stage?

a)

Aminoacyl tRNA bind to A site

b)

Aminoacyl tRNA bind to P site

c)

Release factor bind to A site

d)

Release factor bind to P site

23.

What is the result of transcription?

a)

The synthesis of RNA from protein

b)

The synthesis of protein from RNA

c)

The synthesis of protein from DNA

d)

The synthesis of RNA from DNA

24.

What is the complimentary mRNA sequence to the DNA sequence

5' A-T-T-G-C-A 3'

a)

3' T-A-A-C-G-T 5'

b)

5' U-A-A-C-G-T 3'

c)

3' U-A-A-C-G-U 5'

d)

5' T-A-A-G-C-U 3'

25.

All of the followings are the function of promoter except for

a)

Act as binding site for RNA polymerase

b)

Act as stop signal for transcription

c)

To determine which strand act as template

26.
Translation occurs:
a)
Outside of the Nucleus
b)
Inside of the Nucleus
27.
Translation is crucial to the process of making proteins. Which statement best describes what takes place during translation? 
a)
A copy of chromosomal DNA is created 
b)
Information in mRNA is converted into a sequence of amino acids in a protein 
c)
A RNA copy of a DNA strand is made.
d)
Instructions from DNA in the nucleus are brought to the cytoplasm 
28.
Which defines a codon in DNA or mRNA?
a)
pair of nucleic acid and sugar
b)
pair of phosphate and sugar
c)
three-base code
d)
two-base code
29.
The anticodon of a particular tRNA molecule is 
a)
complementary to the corresponding mRNA codon.
b)
complementary to the corresponding triplet in rRNA.
c)
the part of tRNA that bonds with a specific amino acid.
d)
catalytic, making the tRNA a ribozyme.
30.

What is the start codon?

a)

UAC

b)

TAC

c)

AUG

d)

GUA

31.

The region on mRNA that non as non-coding region

a)

introns

b)

exons

c)

codons

d)

anticodons

32.

What is the result of translation?

a)

The synthesis of RNA from protein

b)

The synthesis of protein from RNA

c)

The synthesis of protein from DNA

d)

The synthesis of RNA from DNA

33.
What would happen if we did not have start codons?
a)
Polypeptide chains would continue to grow
b)
Amino acids would bind to tRNA
c)
Protein synthesis wouldn't occur
d)
mRNA would form a double strand
34.

How many codons are required for one amino acid?

a)

1

b)

2

c)

3

d)

4

35.

How many codons are required for three amino acids?

a)

1

b)

2

c)

3

d)

4

36.
What is occurring in this picture?
a)
Translation
b)
Replication
c)
Translocation
d)
Transcription
37.
Which of these bases does not exist in RNA?
a)
C
b)
T
c)
U
d)
A
38.

Transcribe the following DNA strand: CTG AAT

a)

GAC TTA

b)

CTG AAT

c)

GAC UUA

d)

CUG AAU

39.
How many amino acids are coded for by this sequence of nucleotides:
ATG GGA ACT CCA
a)
4
b)
2
c)
6
d)
12
40.

lacA synthesize enzyme

a)

ß- galactosidase

b)

Transacetylase

c)

Permease

41.
Operons are found in the cells of which of the following types of organisms?
a)
prokaryotes (bacteria)
b)
eukaryotes (plant and animals)
c)
animals only
d)
viruses
42.

Which of the following is not a structural gene?

a)

lac z

b)

lac y

c)

lac a

d)

lac I

43.

What is the inducer in lac operon

a)

Glucose

b)

Maltose

c)

Sucrose

d)

Allolactose

44.

In lac operon, B-galactosidase is encoded by

a)

lacG

b)

lacA

c)

lacZ

d)

lacB

45.

For a repressible operon to be transcribed, which of the following must be true?

a)

A corepressor must be present.

b)

RNA polymerase and the active repressor must be present.

c)

RNA polymerase must bind to the promoter, and the repressor must be inactive and not blocking the promoter region.

d)

RNA polymerase cannot be present, and the repressor must be inactive.

46.

Function of operator in lac operon

a)

Act as binding site for RNA polymerase

b)

Act as binding site for lactose

c)

Act as binding site for repressor protein

47.

In the presence lactose, the repressor protein will bind with

a)

operator region

b)

promoter region

c)

lactose

d)

allolactose

48.

lac operon was proposed by

a)

Meselson and Stahl

b)

Jacob and Monod

c)

Hershey and Chase

d)

Watson and Crick

49.
A protein that binds to the operator and blocks the RNA polymerase
a)
regulator
b)
repressor
c)
activator
d)
corepressor
50.

What is a gene?

a)

A segment of RNA that encodes for a protein

b)

The portion of the chromosome that does not get transcribed

c)

A segment of DNA that encodes for a protein

d)

The collection of your chromosomes

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