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WorksheetsURINE SAMPLE PROCESSING
Total questions: 33
Worksheet time: 21mins
These are the type of urine sample, EXCEPT
Mid-stream urine
Catheterized urine
Bag urine
Suprapubic aspirate urine
Swabbed urine
Choose the related criteria for urine sample rejection
Leaking container
The specimen is not urine
Specimen received in inappropriate container
Hemolyzed sample
The sample processing of urine specimen includes .. ?
Urine full examination microscopic examination (FEME)
Biochemical test
Urine culture
Urine sample collection
Why do we do urine testing in clinical microbiology lab?
To diagnose UTI
To identify the sources of UTI
To identify the transmission of UTI
To allow research of UTI
What is Urinary Tract Infection (UTI) ?
Presence of microorganisms in the urinary tract
Presence of blood in the urinary tract
Presence of cell debris in the urinary tract
Presence of urine in the urinary tract
These are the common bacteria that can cause UTI, EXCEPT
Staphylococcus Aureus
Escherichia Coli
Pseudomonas Aeruginosa
Candida Albicans
Name one organism that could cause UTI
The urinary system is usually sterile, any presence of microorganism there could indicate UTI. Choose the STERILE parts of urinary tract.
Urethra
Urinary bladder
Ureter
Kidney
How can we diagnose someone with UTI?
Clinical symptoms
Positive lab result
Negative lab result
Fever
Why we must avoid delay when transporting urine specimen?
Avoid microorganism multiply after
Avoid hemolysis of urine
Avoid producing bladder stones
Allow natural culturing method
If delay is not avoidable during transportation of urine specimen, how can we preserve the specimen?
Refrigerate at 4'C
Cryo refrigerate
Use boric acid preservative
Use EDTA
How can we describe the appearance of urine during FEME?
Color of the urine
Volume of the urine
Turbidity
pH of the urine
If the urine is red and cloudy, what is the possible cause of it?
Bacteria infection
Yeast infection
Hemoglobinuria
Hepatocellular jaundice
During FEME, the microscopic examination of urine can show .. ?
WBC
RBC
Crystals
Bacteria
Casts
Choose the CORRECT importance of microscopic examination of urine specimen.
Provide presumptive diagnosis of UTI
Determine pyuria or bacterial infection
Determine how long the patient had suffered from UTI
Provide further clue about consequences of UTI
What do we do on Day-1 of urine culture?
Culture on CLED agar
Count colonies on CLED agar
Check growth on BA, MAC
AST
We only culture urine sample on BA and MAC if .. ?
Bacteria are present
Pus cells ≥ 10/hpf
RBC are present
Casts are present
What is CLED agar?
Selective media - detect and isolate of E.Coli and coliform bacteria in urine
Enrichment media - detect and isolate of E.Coli and coliform bacteria in urine
Selective media - inhibit the growth of E.Coli and coliform bacteria in urine
Enrichment media - inhibit the growth of E.Coli and coliform bacteria in urine
What are the advantages of using CLED agar for urine culture?
Gives clear colonial differentiation
Gives clear diagnostic characteristics.
Supports the growth of S.pyogenes and other fastidious organisms that do not require blood
Support the detection of casts and crystals in urine
We can report significant bacteriuria when .... estimated found in urine.
Less than 10,000/mL
10,000-100,000/mL
More than 100,000/mL
When we report a significant bacteriuria, this indicate
urinary infection
infection or contamination
contamination
What can we see on CLED agar after incubate overnight (Day-2) ?
Mixed growth
No growth
Significant growth
Contamination
If we see a significant growth on CLED agar after overnight incubation (Day-2), what do we do?
Repeat procedure
Identification and biochemical test
AST
Report to physician
This is growth seen on CLED agar.
Large, yellow or yellowish-white colonies.
Highly mucoid and elevated.
What is this microorganism?
Klebsiella spp.
Enterobacter spp.
Pseudomonas spp.
Streptococcus spp.
This is a microorganism on CLED agar. Yellow-ish, in colonies. What is this microorganism?
E. Coli
K. Pneumoniae
S. Aureus
S. Faecalis
Lactose fermenter will appear ... on CLED agar.
Yellow
Blue
Green
Red
Non- Lactose fermenter will appear ... on CLED agar.
Yellow
Blue
Green
Red
This is a microorganism on CLED agar. What is this microorganism?
E. Coli
K. Pneumoniae
Candida Albican
S. Aureus
This is microorganism on BA.
Gray-ish white colonies, smooth, round
Beta hemolysis.
What is this microorganism?
E. Coli
S. Aureus
S. Pyogenes
K. Pneumoniae
This is a microorganism on MacConkey Agar.
Mucoid, pink colonies
What is this microorganism?
S. Aureus
Candida Albicans
K. Pneumoniae
P. Aeruginosa
This is a microorganism on MacConkey agar.
Flat and smooth colonies. Colorless.
What is this microorganism?
P. Aeruginosa
E. Coli
Candida Albicans
K. Pneumoniae
This is microorganism on BA.
Mucoid colony, smooth in shape.
Thick hemolysis zone ; Beta hemolysis
What is this microorganism?
P. Aeruginosa
K. Pneumoniae
Candida Albicans
E. Coli
Interpret the result obtained on CLED agar after overnight incubation
Lactose fermentation occur
No lactose fermentation occur
Hemolysis occur
No hemolysis occur
