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WorksheetsProtein Synthesis Review (AP)
Total questions: 71
Worksheet time: 25mins
Click on all the different names for protein synthesis
Gene expression
Initiation & Termination
Central dogma
Transcription & Translation
rRNA, mRNA, and tRNA
Click on all the parts of a nucleotides of RNA
ribose
deoxyribose
phosphate
base: thymine, uracil, adenine, guanine
base: cytosine, guanine, uracil, adenine
Which RNA carries information from DNA to the ribosome?
rRNA
tRNA
mRNA
sRNA
Which RNA makes up ribosomes?
rRNA
tRNA
mRNA
sRNA
Which RNA carries amino acids to the ribosome?
rRNA
tRNA
mRNA
sRNA
Click on all the words that are used to describe the DNA strand that is used to make mRNA
Template strand
Noncoding strand
Minus strand
Antisense strand
Which of the following are the 3 steps of transcription, in order?
codon recognition, elongation, termination
initiation, peptide bond formation, termination
initiation, elongation, termination
codon recognition, peptide bond formation, translocation
What is the name of the promoter sequence that RNA polymerase binds to?
(a)
What is the name of the subunit in prokaryotes that recognizes the promoter sequence and binds to it to initiate transcription
(a)
In eukaryotes, _____________ binds to the promoter sequence, and then RNA polymerase binds and forms the ___________________________
sigma, transcription initiation complex
transcription factors, transcription initiation complex
transcription initiation complex, transcription factors
sigma, transcription factors
True or False: eukaryotes have 3 RNA polymerases, and prokaryotes have one
True
False
True or False: Once RNA polymerase binds to the DNA strand, the transcription factors & sigma subunit detach
True
False
True or False: RNA polymerase needs a primer to start transcription
True
False
When RNA polymerase reaches the ___________, it detaches and ends transcription
terminator sequence
ending sequence
stop codon
stop sequence
In eukaryotes, the mRNA transcript that is made is called ________________ and will be modified to become _______________
baby mRNA, adult mRNA
pre-mRNA, post-mRNA
mRNA, tRNA
pre-mRNA, mature mRNA
The 5' end of the mRNA gets a ____________ and the 3' end gets a _______________
GTP cap, poly-A tail
ATP cap, poly-T tail
poly-A tail, GTP cap
poly-A tail, ATP cap
Nocoding segments of mRNA are called
(a)
The process of removing introns is known as _________ and is done by protein complexes called ___________, which are made of proteins and RNA molecules called __________
RNA splicing, spliceosomes (snRNPs), snRNA
Exon splicing, snRNA, snRNPs
Intron splicing,
snRNA, spliceosomes
spliceosomes, snRNA, splicers
Which of the following are NOT post-transcriptional modifications?
5' cap
intron removal
poly-A tail
TATA box
During transcription, what is the relationship between DNA and RNA?
Template DNA is the noncoding strand, RNA reads DNA in 3' -> 5' direction
Template DNA is the coding strand, RNA reads DNA in 3' -> 5' direction
Template DNA is the noncoding strand, RNA reads DNA in 5' -> 3' direction
Template DNA is the coding strand, RNA reads DNA in 5' -> 3' direction
What's the name of the process where different mRNA sequences (and therefore different proteins) can be made because of the way the exons are put together?
translation
RNA excising
alternative splicing
Elongation
True or False: In prokaryotes, translation can start before transcription is done
True
False
Initiation by sigma subunits occurs in
prokaryotes
eukaryotes
RNA processing happens in
prokaryotes
eukaryotes
During translation, mRNA is used to create which of the following (click all that apply)
proteins
polypeptides
a chain of amino acids
tRNA and rRNA
During the initiation step of translation, mRNA binds to the ______ of the small ribosomal subunit
rRNA
tRNA
mRNA
snRNA
Once the mRNA & small ribosomal subunit bind together, the small subunit moves along the mRNA until it reaches the
promoter sequence
initiator region
promoter region
start codon
What's the name of the first tRNA that brings an amino acid to the ribosome
(a)
The large ribosomal subunit binds to the small one that has the mRNA & initiator tRNA on it. All together, they now form the
functional protein complex
translation initiation complex
endomembrane system
initiation factor complex
What is the name of the proteins that bring all the components of the translation initiation complex together?
transcription factors
translation factors
initiation factors
start codon factors
The initiator tRNA fits into the ___ site of the ribosome, and the ___ site is free for the next tRNA
P, A
A, P
E, P
P, E
During the elongation step of translation, amino acids are linked together by (a) bonds
Peptide bonds between the amino acids form through the process of
hydrolysis
dehydration synthesis
transcription
codon recognition
True or False: Translation proceeds along the mRNA in a 3' to 5' direction
True
False
When tRNA binds to the mRNA, it attaches itself through its (a) which are complementary to mRNA's bases
When a new tRNA brings the next amino acid, it enters the ___ site of the ribosome
A
P
E
G
As the amino acid chain grows longer, the polypeptide in the ___ site forms a peptide bond with the amino acid in the ___ site.
P, A
A, P
E, P
P, E
After the peptide bond is formed, the tRNA in the ___ site leaves the ribosome
A
P
E
G
The ribosome then moves the tRNA in the A site to the P site, and the A site is now open for the next tRNA to bind. This process is called
codon recognition
peptide bond formation
termination
translocation
Termination of translation occurs when
a stop codon reaches the A site
a stop codon reaches the P site
the ribosome reaches the termination sequence
the ribosomes reaches the end sequence
When the stop codon reaches the A site, the A site accepts a protein called a ________, which causes hydrolysis to happen, releasing the polypeptide
termination factor
release factor
initiation factor
transcription factor
After the polypeptide is released, the protein folds into a 3D shape based on the amino acids'
R groups
carboxyl groups
hydroxyl groups
central carbons
Most proteins are able to fold on their own, but some require helper molecules called
(a)
Sometimes, these molecules are added to the protein to direct it to its correct destination
chaperones
transcription factors
signaling sequence
endomembranes
True or False: Free ribosomes, which are located in the cytoplasm, mostly make proteins that function in the cytoplasm
True
False
Ribosomes attached to the Rough ER make proteins that are transported to the _______ & leave through the cell membrane. This is known as the _________ system
mitchondria, powerhouse
lysosome, exocytosis
vacuole,
storage
golgi,
endomembrane
Transcription or Translation:
Occurs in the nucleus
Transcription
Translation
Transcription or Translation:
Uses initiation factors
Transcription
Translation
Transcription or Translation:
Uses spliceosomes
Transcription
Translation
Transcription or Translation:
Starts at the TATA box
Transcription
Translation
Transcription or Translation:
Uses anticodons
Transcription
Translation
Transcription or Translation:
Ends with the stop codon
Transcription
Translation
Retroviruses (RNA viruses) uses a process called (a) to transcribe its RNA to DNA, and then this DNA is inserted into the host cell's DNA to transcribe & translate more viruses
What are physical/chemical factors (like chemical, tobacco, UV radiation, and pollution) that cause mutations called?
(a)
True or False: DNA polymerase proofreads itself and fixes mistakes that it makes
True
False
Which type of mutation has no effect on the protein/phenotype?
missense
silent
nonsense
insertion
Which type of mutation creates one different amino acid?
insertion
nonsense
insertion
missense
Which type of mutation creates a stop codon?
silent
insertion
nonsense
missense
Click on all the different types of frameshift mutations
substitution
nonsense
insertion
missense
deletion
A mutation where entire genes are rearranged on a chromosome is called
duplication
inversion
translocation
deletion
What is the name of the mutation where genes on one chromosome are swapped with genes on a completely different chromosome?
(a)
Organisms that inherit more than 2 sets of chromosomes are called
polyploid
multiploid
manyploid
megaploid
Mutations found in what type of cells will be passed down to offspring?
somatic cells
gametes
True or False:
Mutations can provide genetic variation that is advantageous for survival and are selected for in natural selection
True
False
Which of the following provides genetic variation for prokaryotes?
mutations
sexual reproduction
meiosis
random mating
When prokaryotes take in DNA that was released by another prokaryote, this is called
conjugation
transformation
transposition
transduction
True or False:
In conjugation, a virus moves DNA from one bacteria to another
True
False
What's it called when DNA is transferred between two bacteria through their pili?
transposition
conjugation
transduction
transformation
What's it called when a DNA segment is either moved within itself or into another DNA molecule?
(a)
Click on all the things that are true about genetic variation in viruses
Similar viruses can combine their genetic info when they infect the same host
New strains of viruses can be created when viruses combine together in a host
Viruses mutate a a high rate, resulting in new genetic strains of viruses
Viruses reproduce at a fast rate, which can lead to more mutations and recombination
Which of the following is not true about mutations and cancer?
Mutations in tumor suppressor genes can cause cancer
Proto-oncogenes, which regulate the cell cycle, can mutate into oncogenes, which cause cancer
Mutations in gametes usually lead to cancer
Mutations in proteins that make sure the cell doesn't divide uncontrollably can lead to cancer
