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Protein Synthesis Review (AP)

Total questions: 71

Worksheet time: 25mins

Name
Class
Date
1.

Click on all the different names for protein synthesis

a)

Gene expression

b)

Initiation & Termination

c)

Central dogma

d)

Transcription & Translation

e)

rRNA, mRNA, and tRNA

2.

Click on all the parts of a nucleotides of RNA

a)

ribose

b)

deoxyribose

c)

phosphate

d)

base: thymine, uracil, adenine, guanine

e)

base: cytosine, guanine, uracil, adenine

3.

Which RNA carries information from DNA to the ribosome?

a)

rRNA

b)

tRNA

c)

mRNA

d)

sRNA

4.

Which RNA makes up ribosomes?

a)

rRNA

b)

tRNA

c)

mRNA

d)

sRNA

5.

Which RNA carries amino acids to the ribosome?

a)

rRNA

b)

tRNA

c)

mRNA

d)

sRNA

6.

Click on all the words that are used to describe the DNA strand that is used to make mRNA

a)

Template strand

b)

Noncoding strand

c)

Minus strand

d)

Antisense strand

7.

Which of the following are the 3 steps of transcription, in order?

a)

codon recognition, elongation, termination

b)

initiation, peptide bond formation, termination

c)

initiation, elongation, termination

d)

codon recognition, peptide bond formation, translocation

8.

What is the name of the promoter sequence that RNA polymerase binds to?

(a)  

9.

What is the name of the subunit in prokaryotes that recognizes the promoter sequence and binds to it to initiate transcription

(a)  

10.

In eukaryotes, _____________ binds to the promoter sequence, and then RNA polymerase binds and forms the ___________________________

a)

sigma, transcription initiation complex

b)

transcription factors, transcription initiation complex

c)

transcription initiation complex, transcription factors

d)

sigma, transcription factors

11.

True or False: eukaryotes have 3 RNA polymerases, and prokaryotes have one

a)

True

b)

False

12.

True or False: Once RNA polymerase binds to the DNA strand, the transcription factors & sigma subunit detach

a)

True

b)

False

13.

True or False: RNA polymerase needs a primer to start transcription

a)

True

b)

False

14.

When RNA polymerase reaches the ___________, it detaches and ends transcription

a)

terminator sequence

b)

ending sequence

c)

stop codon

d)

stop sequence

15.

In eukaryotes, the mRNA transcript that is made is called ________________ and will be modified to become _______________

a)

baby mRNA, adult mRNA

b)

pre-mRNA, post-mRNA

c)

mRNA, tRNA

d)

pre-mRNA, mature mRNA

16.

The 5' end of the mRNA gets a ____________ and the 3' end gets a _______________

a)

GTP cap, poly-A tail

b)

ATP cap, poly-T tail

c)

poly-A tail, GTP cap

d)

poly-A tail, ATP cap

17.

Nocoding segments of mRNA are called

(a)  

18.

The process of removing introns is known as _________ and is done by protein complexes called ___________, which are made of proteins and RNA molecules called __________

a)

RNA splicing, spliceosomes (snRNPs), snRNA

b)

Exon splicing, snRNA, snRNPs

c)

Intron splicing,

snRNA, spliceosomes

d)

spliceosomes, snRNA, splicers

19.

Which of the following are NOT post-transcriptional modifications?

a)

5' cap

b)

intron removal

c)

poly-A tail

d)

TATA box

20.

During transcription, what is the relationship between DNA and RNA?

a)

Template DNA is the noncoding strand, RNA reads DNA in 3' -> 5' direction

b)

Template DNA is the coding strand, RNA reads DNA in 3' -> 5' direction

c)

Template DNA is the noncoding strand, RNA reads DNA in 5' -> 3' direction

d)

Template DNA is the coding strand, RNA reads DNA in 5' -> 3' direction

21.

What's the name of the process where different mRNA sequences (and therefore different proteins) can be made because of the way the exons are put together?

a)

translation

b)

RNA excising

c)

alternative splicing

d)

Elongation

22.

True or False: In prokaryotes, translation can start before transcription is done

a)

True

b)

False

23.

Initiation by sigma subunits occurs in

a)

prokaryotes

b)

eukaryotes

24.

RNA processing happens in

a)

prokaryotes

b)

eukaryotes

25.

During translation, mRNA is used to create which of the following (click all that apply)

a)

proteins

b)

polypeptides

c)

a chain of amino acids

d)

tRNA and rRNA

26.

During the initiation step of translation, mRNA binds to the ______ of the small ribosomal subunit

a)

rRNA

b)

tRNA

c)

mRNA

d)

snRNA

27.

Once the mRNA & small ribosomal subunit bind together, the small subunit moves along the mRNA until it reaches the

a)

promoter sequence

b)

initiator region

c)

promoter region

d)

start codon

28.

What's the name of the first tRNA that brings an amino acid to the ribosome

(a)  

29.

The large ribosomal subunit binds to the small one that has the mRNA & initiator tRNA on it. All together, they now form the

a)

functional protein complex

b)

translation initiation complex

c)

endomembrane system

d)

initiation factor complex

30.

What is the name of the proteins that bring all the components of the translation initiation complex together?

a)

transcription factors

b)

translation factors

c)

initiation factors

d)

start codon factors

31.

The initiator tRNA fits into the ___ site of the ribosome, and the ___ site is free for the next tRNA

a)

P, A

b)

A, P

c)

E, P

d)

P, E

32.

During the elongation step of translation, amino acids are linked together by (a)   bonds

33.

Peptide bonds between the amino acids form through the process of

a)

hydrolysis

b)

dehydration synthesis

c)

transcription

d)

codon recognition

34.

True or False: Translation proceeds along the mRNA in a 3' to 5' direction

a)

True

b)

False

35.

When tRNA binds to the mRNA, it attaches itself through its (a)   which are complementary to mRNA's bases

36.

When a new tRNA brings the next amino acid, it enters the ___ site of the ribosome

a)

A

b)

P

c)

E

d)

G

37.

As the amino acid chain grows longer, the polypeptide in the ___ site forms a peptide bond with the amino acid in the ___ site.

a)

P, A

b)

A, P

c)

E, P

d)

P, E

38.

After the peptide bond is formed, the tRNA in the ___ site leaves the ribosome

a)

A

b)

P

c)

E

d)

G

39.

The ribosome then moves the tRNA in the A site to the P site, and the A site is now open for the next tRNA to bind. This process is called

a)

codon recognition

b)

peptide bond formation

c)

termination

d)

translocation

40.

Termination of translation occurs when

a)

a stop codon reaches the A site

b)

a stop codon reaches the P site

c)

the ribosome reaches the termination sequence

d)

the ribosomes reaches the end sequence

41.

When the stop codon reaches the A site, the A site accepts a protein called a ________, which causes hydrolysis to happen, releasing the polypeptide

a)

termination factor

b)

release factor

c)

initiation factor

d)

transcription factor

42.

After the polypeptide is released, the protein folds into a 3D shape based on the amino acids'

a)

R groups

b)

carboxyl groups

c)

hydroxyl groups

d)

central carbons

43.

Most proteins are able to fold on their own, but some require helper molecules called

(a)  

44.

Sometimes, these molecules are added to the protein to direct it to its correct destination

a)

chaperones

b)

transcription factors

c)

signaling sequence

d)

endomembranes

45.

True or False: Free ribosomes, which are located in the cytoplasm, mostly make proteins that function in the cytoplasm

a)

True

b)

False

46.

Ribosomes attached to the Rough ER make proteins that are transported to the _______ & leave through the cell membrane. This is known as the _________ system

a)

mitchondria, powerhouse

b)

lysosome, exocytosis

c)

vacuole,

storage

d)

golgi,

endomembrane

47.

Transcription or Translation:

Occurs in the nucleus

a)

Transcription

b)

Translation

48.

Transcription or Translation:

Uses initiation factors

a)

Transcription

b)

Translation

49.

Transcription or Translation:

Uses spliceosomes

a)

Transcription

b)

Translation

50.

Transcription or Translation:

Starts at the TATA box

a)

Transcription

b)

Translation

51.

Transcription or Translation:

Uses anticodons

a)

Transcription

b)

Translation

52.

Transcription or Translation:

Ends with the stop codon

a)

Transcription

b)

Translation

53.

Retroviruses (RNA viruses) uses a process called (a)   to transcribe its RNA to DNA, and then this DNA is inserted into the host cell's DNA to transcribe & translate more viruses

54.

What are physical/chemical factors (like chemical, tobacco, UV radiation, and pollution) that cause mutations called?

(a)  

55.

True or False: DNA polymerase proofreads itself and fixes mistakes that it makes

a)

True

b)

False

56.

Which type of mutation has no effect on the protein/phenotype?

a)

missense

b)

silent

c)

nonsense

d)

insertion

57.

Which type of mutation creates one different amino acid?

a)

insertion

b)

nonsense

c)

insertion

d)

missense

58.

Which type of mutation creates a stop codon?

a)

silent

b)

insertion

c)

nonsense

d)

missense

59.

Click on all the different types of frameshift mutations

a)

substitution

b)

nonsense

c)

insertion

d)

missense

e)

deletion

60.

A mutation where entire genes are rearranged on a chromosome is called

a)

duplication

b)

inversion

c)

translocation

d)

deletion

61.

What is the name of the mutation where genes on one chromosome are swapped with genes on a completely different chromosome?

(a)  

62.

Organisms that inherit more than 2 sets of chromosomes are called

a)

polyploid

b)

multiploid

c)

manyploid

d)

megaploid

63.

Mutations found in what type of cells will be passed down to offspring?

a)

somatic cells

b)

gametes

64.

True or False:

Mutations can provide genetic variation that is advantageous for survival and are selected for in natural selection

a)

True

b)

False

65.

Which of the following provides genetic variation for prokaryotes?

a)

mutations

b)

sexual reproduction

c)

meiosis

d)

random mating

66.

When prokaryotes take in DNA that was released by another prokaryote, this is called

a)

conjugation

b)

transformation

c)

transposition

d)

transduction

67.

True or False:

In conjugation, a virus moves DNA from one bacteria to another

a)

True

b)

False

68.

What's it called when DNA is transferred between two bacteria through their pili?

a)

transposition

b)

conjugation

c)

transduction

d)

transformation

69.

What's it called when a DNA segment is either moved within itself or into another DNA molecule?

(a)  

70.

Click on all the things that are true about genetic variation in viruses

a)

Similar viruses can combine their genetic info when they infect the same host

b)

New strains of viruses can be created when viruses combine together in a host

c)

Viruses mutate a a high rate, resulting in new genetic strains of viruses

d)

Viruses reproduce at a fast rate, which can lead to more mutations and recombination

71.

Which of the following is not true about mutations and cancer?

a)

Mutations in tumor suppressor genes can cause cancer

b)

Proto-oncogenes, which regulate the cell cycle, can mutate into oncogenes, which cause cancer

c)

Mutations in gametes usually lead to cancer

d)

Mutations in proteins that make sure the cell doesn't divide uncontrollably can lead to cancer