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WorksheetsCalcium and Magnesium
Total questions: 90
Worksheet time: 2hrs 30mins
Calcium is the _ most common element
3rd
4th
5th
6th
It is the most prevalent cation in the body
Calcium
Magnesium
Bicarbonate
6th
Calcium in blood is only _ of the total calcium in the body.
0.5%
1.0%
1.5%
11%
_ of calcium is found in the bone.
55%
66%
77%
88%
99%
Which of the following are the functions of Calcium?
Bone mineralization
Blood coagulation
Neural transmission and Hormonal secretion
Muscle contraction
Cardiac contractility and conduction
Of this 1% Calcium in the blood it has three forms:
- 40% as ionized Ca+ or the free form of calcium
- 45% bound to protein (albumin)
- 15% bound to anions (Citrate, HCO3-, Lactate)
True
False
The form of calcium that is detected by the glands is the?
Ionized Ca+
Ca+ bound to protein (albumin)
Ca+ bound to anions
Ca+ in bones
When there is a low concentration of calcium in the blood, this would trigger the release of what hormone?
Parathyroid hormone
Growth Hormone
Aldosterone
Antidiuretic hormone
The parathyroid hormone will act on what organ/s to increase calcium absorption?
Bone
Kidneys
Brain
Pituitary gland
In the bone, it promotes bone resorption while in the kidneys, it promotes tubular reabsorption of calcium; therefore, leading to an increase in the calcium levels.
First: True
Second: False
First: False
Second: True
Both True
Both False
Vitamin D is obtained from sunlight in the form of 8-dehydro-cholesterol.
True
False
7-dehydro-cholesterol will be converted into Pre-vitamin D3 and then into vitamin D and then this Vitamin D will be hydroxylated by the 25-hydroxylase enzyme from the liver into 25- hydroxycholecalciferol (25-OH-D).
True
False
This 25-OH-D would be hydroxylated by 1α-hydroxylase into 1,25-dihydroxycholecalciferol or 1,25-(OH)2-D which is the active form of vitamin D.
True
False
This 1αhydroxylase is being influenced by the release of the parathyroid hormone. So, when parathyroid hormone is released, this would activate the kidneys to secrete 1αhydroxylase to convert the inactive form of vitamin D (25-OH-D) into its active form which is 1,25-(OH)2-D. And this is the one (1,25-(OH)2-D) responsible for the absorption of calcium ions in the kidneys and in the intestines.
True
False
Calcitonin: Is inhibitory to PTH and Vitamin C
True
False
Hormone responsible for decreasing calcium levels
Growth Hormone
Parathyroid Hormone
Aldosterone
Calcitonin
Luteinizing Hormone
When there is an increase in the ionized calcium levels, this would trigger the release of calcitonin from the thyroid gland and will have the following actions: there will be a increase in intestinal absorption of calcium ions, there will be a increase in bone resorption and on the kidneys, there will be increase in calcium reabsorption. So, the total effect of calcitonin is to have a increase in calcium levels.
First: True
Second: False
First: False
Second: True
Both True
Both False
Calcium is regulated by three substance/hormones, namely, parathyroid hormone, vitamin C, and aldosterone.
True
False
The form of calcium that is measured is total calcium and the ionized form of calcium.
True
False
Colorimetric Method: Calcium binds with the reagent or the dye to form the colored complex (AR complex) and this will be the one that is measured spectrophotometrically and a decrease in the absorbance is directly proportional with the ion concentration.
True
False
The dye used in the experiment is?
(a)
8-Hydroxyquinoline is used to prevent or remove any interference from (a) ions.
Another dye for the experiment that can be used is?
(a)
What is the reference method of Total Calcium Determination?
(a)
In Ion Selective Electrode, within the electrode there are membranes inside incorporated with Copper chloride which selectively binds to calcium ions. An electron potential will develop inside which is inversely proportional to calcium ions.
First: True
Second: False
First: False
Second: True
Both True
Both False
Which of the following are the content of your Calcium Reagent Kit:
8-Hydroxyquinoline
O-cresolphthalein complexone
Hydrochloric acid
Sodium azide
Lysine buffer
What is the pH of Lysine buffer in Total Calcium Determination?
9.1
6.1
11.1
4.1
In Total Calcium Determination, Sodium azide acts as your?
Stabilizer
Potentiatior
Standard
Buffer
In Total Calcium Determination the preferred sample is/are?
Serum
Heparinized plasma
CSF
Hemolyzed sample
EDTA
Relationship of Calcium to EDTA and Oxalate
Falsely Increase
Falsely Decreased
NOTA
Insufficient information
In Ionized Calcium Determination, samples must be collected anaerobically?
True
False
In Ionized Calcium Determination, Serum collected in sealed evacuated blood tubes at freezing temperature.
True
False
What blank was used in Calcium Determination?
Reagent Blank
Air Blank
Water Blank
Serum Blank
What is the volume of the Standard, Control Normal, Control Pathologic, and Unknown in Calcium Determination.
0.02 mL
0.01mL
0.2 mL
0.1 mL
What is the volume of working reagent used in Calcium Determination?
1.0 mL
10.0 mL
2.0 mL
0.5 mL
In Calcium determination, what the suitable time frame in reading the absorbance of the sample and standard against the reagent blank?
5 to 20 mins
5 to 50 mins
5 to 15 mins
5 to 30 mins
In Calcium determination, what the suitable time frame in reading the absorbance of the sample and standard against the reagent blank?
5 to 20 mins
5 to 50 mins
5 to 15 mins
5 to 30 mins
In Calcium Determination, the absorbance of the sample will be divided by the absorbance in the standard, and the quotient will be multiplied by the concentration of the standard.
True
False
In Calcium Determination, the conversion factor: multiply 0.25 by mg/dL = mmol/L
True
False
The reference range of Children <12 years for Total Calcium in serum and plasma
2.20-2.70 mmol/L (8.8 - 10.8 mg/dL)
2.15-2.50 mmol/L (8.6 - 10.0 mg/dL)
1.20–1.38 mmol/L (4.8 – 5.5 mg/dL)
1.16–1.32 mmol/L (4.6–5.3 mg/dL)
The reference range of Adults for Total Calcium in serum and plasma
2.20-2.70 mmol/L (8.8 - 10.8 mg/dL)
2.15-2.50 mmol/L (8.6 - 10.0 mg/dL)
1.20–1.38 mmol/L (4.8 – 5.5 mg/dL)
1.16–1.32 mmol/L (4.6–5.3 mg/dL)
The reference range of Adults for Ionized Calcium in serum
2.20-2.70 mmol/L (8.8 - 10.8 mg/dL)
2.15-2.50 mmol/L (8.6 - 10.0 mg/dL)
1.20–1.38 mmol/L (4.8 – 5.5 mg/dL)
1.16–1.32 mmol/L (4.6–5.3 mg/dL)
The reference range of Children for Ionized Calcium in serum
2.20-2.70 mmol/L (8.8 - 10.8 mg/dL)
2.15-2.50 mmol/L (8.6 - 10.0 mg/dL)
1.20–1.38 mmol/L (4.8 – 5.5 mg/dL)
1.16–1.32 mmol/L (4.6–5.3 mg/dL)
The reference range of Adult for Ionized Calcium in plasma
1.03 – 1.23 mmol/L (4.1 – 4.9 mg/dL)
1.15 – 1.27 mmol/L (4.6 – 5.1 mg/dL)
1.20–1.38 mmol/L (4.8 – 5.5 mg/dL)
1.16–1.32 mmol/L (4.6–5.3 mg/dL)
What is the reference range for Total Calcium in urine 24h
2.50-7.50 mmol/day (100-300 mg/day)
5-10 mmol/day
(200-400 mg/day)
7.50-15.0 mmol/day (300-600 mg/day)
0-10.0 mmol/day (0-400 mg/day)
Hypercalcemia is primarily caused hypoparathyroidism
True
False
In hyperthyroidism, an increase in the activity of the thyroid gland will also lead to an increase in the activity of the parathyroid gland
True
False
Benign familial hypocalciuria
Falsely Increased Calcium Levels
Falsely Decreased Calcium Levels
In Malignancy (PTH-rp), the tumor cells will secrete parathyroid hormone-related peptide which acts like a parathyroid hormone which will lead to hypocalcemia
True
False
In Multiple Myeloma, there is a decrease in bone resorption.
True
False
Increased vitamin D will lead to more absorption of calcium ions.
True
False
Thiazide diuretics will lead to increase in tubular reabsorption of calcium
True
False
Prolonged immobilization is one of the causes of hypocalcemia?
True
False
All are causes of hypocalcemia except:
Primary Hypoparathyroidism
Acute Pancreatitis
Renal Diseases
Rhabdomyolysis
Malignancy (PTH-rp)
In primary hypoparathyroidism, a decrease in parathyroid hormone will lead to decreased absorption of calcium ions.
True
False
In hypoalbuminemia, the decrease of albumin levels will lead to increase in calcium ions
True
False
In acute pancreatitis, There is an increase in the levels of the lipase, and lipase releases more free fatty acids, and when there are more free fatty acids released in the circulation, this will bind the calcium ions in the circulation, therefore leading to hypercalcemia
True
False
Vitamin D deficiency will lead to a decrease in the absorption of calcium ions.
True
False
In renal disease, there will be an active form of vitamin D that will be formed therefore leading to hypocalcemia.
True
False
In rhabdomyolysis, there will be an increase in the release of phosphate in the cells which will bind the calcium ions in circulation, therefore leading to hypocalcemia
True
False
In Pseudohypoparathyroidism, there is an end organ resistance. So, the target tissues do not respond to the actions of PTH
True
False
It is the 4th most abundant cation in the body
Magnesium
Calcium
Phosphate
Bicarbonate
2nd most abundant intracellular ion
Magnesium
Calcium
Phosphate
Bicarbonate
All of the following are functions of Magnesium.
Cofactor of Cellular Enzymes and DNA replication and transcription
Cellular Energy Metabolism
Membrane Stabilization
Ion Transport
Nerve Conduction
Which are correctly matched in the distribution of magnesium?
53% - bone
46% - muscle, other organs, and soft tissues
<1% - serum, RBCs
1/3 of the <1% is bound to proteins (albumin)
2/3 of the <1% is 61% ionized state and 5% is complexed to other ions (PO4- and citrate)
What organ is responsible for the overall regulation of Magnesium? (reabsorption and excretion)
Kdineys
Intestines
Lungs
Bones
Aldosterone and Thyroxine decrease renal excretion of Mg++
True
False
PTH increases renal and intestinal Mg++ reabsorption
True
False
In renal absorption: Which of the following is correct?
25-50% in PCT - Proximal Convoluted Tubule
50-60% in Ascending limb
2-5% in DCT - Distal Convoluted Tubule
Majority are absorbed in the ascending limb
In Magnesium Determination, the forms of magnesium that are measured are total form of magnesium and ionized form of magnesium
True
False
In Calmagite Method, the Magnesium ions will bind with the dye to form a red-violet colored complex.
True
False
In Calmagite Method, the absorbance is read at 532 nm
True
False
In Formazan Dye, the absorbance is read at 660 nm
True
False
The Methylthymol blue or Magon or Xylidyl blue is the one being used in the experiment in the laboratory manual?
True
False
The reference method for total magnesium determination?
Atomic Absorption Spectrophotometer
Ion Selective Electrode
Calmagite Method
Formazan Dye
What is the content of the Magnesium Reagent Kit?
Potassium carbonate
GEDTA
Xylidyl blue
Activators
In Magnesium Reagent Kit, GEDTA removes any interferences from the calcium ions?
True
False
What is the buffer and stabilizer used in Magnesium determination?
TRIS buffer
Sodium azide
Magnesium
Lysine buffer
What is the PH of TRIS buffer?
11.0
10.0
10.5
11.5
Anti-coagulant such as EDTA, oxalate and citrate are recommended because this will bind magnesium ions therefore leading to normal ion concentration
True
False
The preferred sample for Magnesium Determination is?
Hemolyzed serum
Lithium heparin plasma
EDTA plasma
Oxalate plasma
Citrate plasma
24 hour urine is preferred because of diurnal variation?
True
False
In Magnesium Determination, the volume of each sample used is?
0.01 mL
0.02 mL
0.1 mL
0.2 mL
In Magnesium Determination, measure the absorbance of the sample and the standard against the reagent blank within 60 minutes
True
False
In Magnesium Determination, the conversion factor is multiplying 0.5 by mEqL = mmol/L
True
False
In Magnesium Determination, the reference range for serum is?
0.63-1.0mmol/L
0.8-1.2mmol/L
0.55-1.1mmol/L
0.7-1.4mmol/L
In Magnesium Determination, the reference range for serum is?
1.26 – 2.10 mEq/L
1.5 – 2.0 mEq/L
1.42 – 2.08 mEq/L
1.0– 1.8 mEq/L
All of the following are causes of Hypermagnesemia except:
Decreased excretion
Increased intake of antacids, enemas, cathartics, therapeutics
Excess lactation
Dehydration
Bone carcinoma
Thyroxine and aldosterone increases the renal excretion of magnesium ions. So, when there is a decrease in these hormones this will lead to a decrease of the excretion of magnesium ions therefore leading to hypermagnesemia.
True
False
All of the following are causes of Hypomagnesemia except:
Reduced Intake
Decrease absorption
Increased excretion
Pregnancy
Dehydration
