Font size
WorksheetsGPB part 1
Total questions: 33
Worksheet time: 17mins
Habitat: ubiquitous: soil, water, dust, vegetation
Bacillus anthracis
Bacillus cereus
Bacillus species
causes disease in cattle, swine and horses
Bacillus anthracis
Bacillus cereus
Bacillus species
Usually large, Gram positive bacilli
Form spores:
terminal/subterminal
swell the cell/do not swell the cell
Catalase positive
Bacillus anthracis
Bacillus cereus
Bacillus species
CSF/Blood Culture:
gram positive bacilli in chains
India ink: capsule present
Bacillus anthracis
Bacillus cereus
Bacillus species
Growth on SBA
large, ground glass, ”medusa head”, nonhemolytic colonies
Bacillus anthracis
Bacillus cereus
Bacillus species
Biochemical test results:
catalase positive
nonmotile
Bacillus anthracis
Bacillus cereus
Bacillus species
Gram stain from thio
large gram positive bacilli with spores that don’t swell the cell
“bamboo” appearance
Bacillus anthracis
Bacillus cereus
Bacillus species
What is the gram stain in the picture of?
Bacillus anthracis
Bacillus cereus
Bacillus species
Inhalation, through the Skin, Ingestion of highly infectious spores
Bacillus anthracis
Bacillus cereus
Bacillus species
Forms of anthrax
”woolsorter’s” disease
Pulmonary anthrax
Cutaneous anthrax
Gastrointestinal anthrax
Forms of anthrax
”malignant pustule”
Pulmonary anthrax
Cutaneous anthrax
Gastrointestinal anthrax
Large, gram + bacilli
Colonies on SBA: large, lavender, feathery, Beta hemolytic
Catalase Positive
Bacillus anthracis
Bacillus cereus
Bacillus species
Opportunistic pathogen: food poisoning from rice, cereals, vegies
Bacillus anthracis
Bacillus cereus
Bacillus species
Cat + / Motile
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Cat -/Nonmotile
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Cat +/Nonmotile
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Weak, B-hemolysis
Grows well at 40C-can isolate by cold enrichment
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Newborns/women
Meningitis
Septicemia
Food poisoning related to meat & milk products
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Catalase +
Bile Esculin +
CAMP test + (block)
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Motility +
Motility Media: incubated at 25 C: umbrella-shape
Wet prep: tumbling motility
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Habitat: small mammals, pigs, fish
Causes erysipeloid, well-defined, red centrally raised skin lesion
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Alpha or Gamma-hemolysis on SBA
Catalase negative
TSI--H2S positive
Non-motile
“Bottle brush” in gelatin deep
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Normal flora of mouth, GI tract, and vagina (maintains pH) (eat yogurt!!)
Colonies on SBA: pinpoint, alpha- hemolytic
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Capnophilic to anaerobic
Gram positive coccobacilli or thin, narrow bacilli; may chain
Catalase negative
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Normal flora of human throat and skin
Common contaminant of blood cultures and wounds
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Gram positive to Gram variable bacilli/coccobacilli, very pleomorphic; often with palisades, club-shapes, and Chinese letter forms
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
“diphtheroids”
Catalase positive
Listeria monocytogenes
Erysipelothrix rhusiopathiae
Lactobacillus species
Corynebacterium
Catalase positive
Nitrate negative
Urease negative
Vancomycin sensitive (resistant to others)
Corynebacterium jeikeium
Corynebacterium diphtheriae
Metachromatic granules (Babes –Ernst bodies): Stain with methylene blue stain
Corynebacterium jeikeium
Corynebacterium diphtheriae
toxigenic strains of Corynebacterium diphtheriae
diphtheria
Tellurite Selective Media
CTBA & Tinsdale agar
Loeffler or Pai agar (enriched)
Elek test (an immunodiffusion
test)
Corynebacterium diphtheriae
tellurite is inhibitory to normal flora
Colonies: black or brown, usually with brown halos and a garlic odor
diphtheria
Tellurite Selective Media
CTBA & Tinsdale agar
Loeffler or Pai agar (enriched)
Elek test (an immunodiffusion
test)
Corynebacterium diphtheriae
contain blood or serum
enahnces typical staining morphology & metachromatic (Babes-Ernst) granules
diphtheria
Tellurite Selective Media
CTBA & Tinsdale agar
Loeffler or Pai agar (enriched)
Elek test (an immunodiffusion
test)
Corynebacterium diphtheriae
in vitro immunoprecipitation (immunodiffusion) test to determine whether or not a strain of Corynebacterium diphtheriae is toxigenic
diphtheria
Tellurite Selective Media
CTBA & Tinsdale agar
Loeffler or Pai agar (enriched)
Elek test (an immunodiffusion
test)
