Bacteria that are metabolically active

The primary stain used during spore staining that stains the spore and not the vegetative cell

Counter or contrasting stain used during spore staining to stain the vegetative cell

Why is heat necessary in spore staining?

This media selects for gram-negative organisms and isolates lactose fermenters (pink) and non-lactose fermenters (clear).

Type of culturing media: Selects for gram-positive bacteria, halophiles; Differentiates on their ability to ferment mannitol

Type of culturing media; Selects for growth of fungi (has a pH of 5.6 and high levels of glucose)

The image describes a _____.

The two most common types of media used in the laboratory are:

CO₂ incubators are used to grow:

This type of complex media is more rich than nutrient agar and includes 5% sheep's blood

The followings are objectives of serial dilutions EXCEPT.

Which of the following is the optimum number of colonies needed to count a bacterial plate?

Hydrogen Peroxide (H2O2) is the reagent in the catalase test

No reaction on the slide during the catalase test means the bacteria is

Mannitol Salt Agar is selective for which bacterial genus?

Which type of media can be used to determine if a bacteria is motile?

Turbidity is measured using a ____

MacConkey agar was used to grow organisms and the agar turned red/fuchsia. This means that:

Which enzyme is tested for by using the indole test? (indole is the product if this enzyme is present)

What component of the endospore coat retains the primary stain during the endospore staining process?

What are two genera that are medically important and form endospores?

Resazurin and Methylene blue change colors based on the presence or absence of ---?

What does CFU stand for?

Fermentation tubes containing glucose were inoculated with bacteria and turned yellow with a bubble in the Durham tube. What does this mean?

When measuring the zone of inhibition (ZOI) for disinfectants, how will you decide which disinfectant is best?

The enzyme that helps to break down starch is...

Two types of bacteria were grown and tested for amylase using iodine. Which bacterial colony is positive?

When analyzed in SIM tubes, if bacteria produce the enzyme that removes sulfur from amino acids, this will appear as

SIM tubes are useful for differentiation of what types of medically important pathogens?

Bacteria were grown on Mannitol salt agar and the agar turned yellow. This means...(choose two answers)

Bacteria were grown on MacConkey agar and the colonies were colorless. This means...

What type of organisms are able to grow on MacConkey agar?

The coagulase test is the standard definitive method of confirming the identification of what pathogenic bacteria?

What type of plates are used for a Kirby-Bauer test?

The Kirby-Bauer method is used to

How is the Kirby-Bauer method different from testing disinfectants?

If 1 mL sample is put into a tube that has 24 ml diluent, what is the dilution you prepared?

You need to make a 1:10 dilution and you need 50 ml of the diluted culture. How much culture do you add to the broth to make 50 mls of the preparation?

You use the membrane filtration method to test 100 ml of your favorite bottle of water for bacterial colonies. You then plated the filter and incubated it. The next day you found 143 colonies. What is the bacterial density of the water?

What is the bacterial concentration if 72 colonies were counted after 1 ml of a 1:10,000 dilution was plated?

What is the bacterial concentration if 56 colonies were counted after 0.5ml of a 1:1,000 dilution was plated?

What is the enzyme that is linked to breaking down blood cells?

When bacteria are capable of fermentation, they are breaking down

When fermentation tubes contain phenol red (as we observed in lab), they appear what color when the pH is ACIDIC?

The disk diffusion test for disinfectants shows the chemical's ability to

When we determine if tryptophan is broken down by bacteria, we detect this product in the SIM tubes

A spectrophotometer does NOT (choose all that apply)

A measurement of bacterial concentration that tells you the specific CFU/ml in a sample is a _______ measurement

Pathogenic Staphylococcus aureus is

What is the difference between nutrient agar and lactose broth?

Blood agar is

What does a completely clear zone around bacteria on a Blood agar plate indicate?

Which of the following is a selective medium that inhibits the growth of gram-positive bacteria?

What is the purpose of using a Durham tube in a fermentation test?

________________________ observations involve information that you can count or measure.

the color of your shoes

What is the selective ingredient in Mannitol Salt Agar (MSA)?

What is a bacterial colony?