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G.B.Sc.N - MICROBIOLOGY-2

Total questions: 40

Worksheet time: 1hrs 20mins

Name
Class
Date
1.

What type of staining technique Gram's staining is?

a)

Differential staining

b)

Negative staining

c)

Selective staining

d)

Simple staining

2.

What type of staining Acid-fast staining is?

a)

Differential staining

b)

Negative staining

c)

Selective staining

d)

Simple staining

3.

Acid-fast staining is also called:

a)

Gram's staining

b)

Hanging drop technique

c)

Negative staining

d)

Ziehl-Neelson staining

4.

It is coloring component of a stain:

a)

Acidic dye

b)

Alcohol

c)

Basic dye

d)

Chromophore

5.

Mycolic acid is present in:

a)

Acid fast Bacteria

b)

Gram Negative Bacteria

c)

Gram Positive Bacteria

d)

Nonacid fast Bacteria

6.

The color of Acid-fast bacteria is:

a)

Blue

b)

Pink

c)

Purple

d)

Red

7.

The color of Non-Acid-fast bacteria is:

a)

Blue

b)

Pink

c)

Purple

d)

Red

8.

The color of Gram negative bacteria is:

a)

Blue

b)

PinK

c)

Purple

d)

Red

9.

The color of Gram positive bacteria is:

a)

Blue

b)

Pink

c)

Purple

d)

Red

10.

Primary dye used in Gram staining is:

a)

Carbol fuchsin

b)

Crystal violet

c)

Methylene blue

d)

Safranin

11.

Secondary dye used in Gram staining is:

a)

Carbol fuchsin

b)

Crystal violet

c)

Methylene blue

d)

Safranin

12.

Secondary dye used in Acid-fast staining is:

a)

Carbol fuchsin

b)

Crystal violet

c)

Methylene blue

d)

Safranin

13.

Primary dye used in Acid-fast staining is:

a)

Carbol fuchsin

b)

Crystal violet

c)

Methylene blue

d)

Safranin

14.

Type of staining in which back groung is colored leaving the bacterial cells colorless is:

a)

Differential staining

b)

Negative staining

c)

Selective staining

d)

Simple staining

15.

Type of staining in which specific component of a bacterial cells is colored is:

a)

Differential staining

b)

Negative staining

c)

Selective staining

d)

Simple staining

16.

Fractional sterilization is also called:

a)

Autoclaving

b)

Desiccation

c)

Pasteurization

d)

Tyndallization

17.

Type of heat used in incineration is:

a)

Dry heat

b)

Low temperature

c)

Moist heat

d)

Refrigeration

18.

This chemical is used as a disinfectant:

a)

Mercuric chloride

b)

Silver nitrate

c)

Soap

d)

Tincture of iodine

19.

Sterilization can be achieved by:

a)

Autoclaving

b)

Boiling

c)

Filtration

d)

Pasteurization

20.

Chemical agents that cannot withstand long period of boiling or autoclaving can be sterilized by:

a)

Autoclaving

b)

Boiling

c)

Desiccation

d)

Fractional sterilization

21.

Working pressure and temperature of autoclave is:

a)

14 psi for 121 º C

b)

15 psi for 120 º C

c)

15 psi for 121 º C

d)

16 psi for 122 º C

22.

Bacteria which require their energy from sunlight are called:

a)

Autotrophs

b)

Chemotrophs

c)

Heterotrophs

d)

Phototrophs

23.

The phase of bacterial growth curve in which rate of cell growth is equal to the death rate of bacterial cells:

a)

Death phase

b)

Lag phase

c)

Log phase

d)

Stationary phase

24.

The phase of bacterial growth curve in which bactetial cells grow exponentially.

a)

Death phase

b)

Lag phase

c)

Log phase

d)

Stationary phase

25.

The phase of bacterial growth curve in which bactetial cells starts dying off.

a)

Death phase

b)

Lag phase

c)

Log phase

d)

Stationary phase

26.

Bacteria which require their energy from chemical reactions are called:

a)

Autotrophs

b)

Chemotrophs

c)

Heterotrophs

d)

Phototrophs

27.

Bacteria which use CO2 or inorganic carbon as their carbon source are called:

a)

Autotrophs

b)

Chemotrophs

c)

Heterotrophs

d)

Phototrophs

28.

Bacteria which use organic carbon as their carbon source are called:

a)

Autotrophs

b)

Chemotrophs

c)

Heterotrophs

d)

Phototrophs

29.

Bacteria whose Carbon source & energy source is inorganic compounds are called:

a)

Chemoautotrophs

b)

Chemoheterotrophs

c)

Photoautotrophs

d)

Photoheterotrophs

30.

Bacteria whose Carbon source & energy source is organic compounds are called:

a)

Chemoautotrophs

b)

Chemoheterotrophs

c)

Photoautotrophs

d)

Photoheterotrophs

31.

Bacteria whose Carbon source is organic carbon & energy source is sunlight are called:

a)

Chemoautotrophs

b)

Chemohetetotrophs

c)

Photoautotrophs

d)

Photohetetotrophs

32.

Bacteria whose Carbon source is inorganic carbon & energy source is sunlight are called:

a)

Chemoautotrophs

b)

Chemohetetotrophs

c)

Photoautotrophs

d)

Photohetetotrophs

33.

Autotrophs are also called:

a)

Heterotrophs

b)

Lithotrophs

c)

Organotrophs

d)

Phototrophs

34.

Heterotrophs are also called:

a)

Autotrophs

b)

Lithotrophs

c)

Organotrophs

d)

Phototrophs

35.

Photoautotrophs are also called:

a)

Chemolithotrophs

b)

Chemoorganotrophs

c)

Photolithotrophs

d)

Photoorganotrophs

36.

Photoheterotrophs are also called:

a)

Chemolithotrophs

b)

Chemoorganotrophs

c)

Photolithotrophs

d)

Photoorganotrophs

37.

Chemoheterotrophs are also called:

a)

Chemolithotrophs

b)

Chemoorganotrophs

c)

Photolithotrophs

d)

Photoorganotrophs

38.

Chemoautotrophs are also called:

a)

Chemolithotrophs

b)

Chemoorganotrophs

c)

Photolithotrophs

d)

Photoorganotrophs

39.

Killing of a bacterial cell is:

a)

Bactericidal

b)

Bacteristatic

c)

Fungicidal

d)

Fungistatic

40.

Inhibiting bacterial growth is:

a)

Algaestatic

b)

Bactericidal

c)

Bacteristatic

d)

Fungistatic