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CLASS_Test01

Total questions: 53

Worksheet time: 2hrs 40mins

Name
Class
Date
1.
The enzyme that unzips the DNA to prepare for replication
a)
helicase
b)
replicase
c)
polymerase
d)
synthase
2.
Which enzyme is responsible for adding nucleotides?
a)
Topoisomerase
b)
DNA Polymerase
c)
Ligase
d)
Primase
3.
Which enzyme seals okazaki fragments together on the lagging strand?
a)
Topoisomerase
b)
Helicase
c)
Polymerase
d)
Ligase
4.

What is A in the given diagram?

a)
Leading strand
b)
Lagging strand
c)
Okazaki Fragments
d)
DNA plymerase
5.

During DNA replication, which strand is copied faster?

a)
Leading
b)
Lagging
6.
Which of the following must happen first in order for DNA replication to occur?
a)
DNA polymerase binds to the leading strand
b)
DNA is unwound
c)
Hydrogen bonds form between bases
d)
chromosomes condense
7.
Which of the following is required for DNA replication to occur?
a)
DNA helicase
b)
DNA ligase
c)
DNA polymerase
d)
all of these
8.

Structure C is

a)

helicase

b)

primase

c)

DNA polymerase III

d)

gyrase

9.
The new DNA on the leading strand is synthesized in the _________ direction
a)
5' to 3'
b)
3' to 5'
10.
The enzyme that removes RNA Primers and fills in the gaps with DNA Nucleotides is
a)
DNA Polymerase III
b)
DNA Polymerase I
c)
DNA Polymerase II
d)
Primase
11.

The enzyme that joins together small segments of newly replicated DNA is:

a)

DNA Polymerase III

b)

DNA Polymerase I

c)

DNA Polymerase II

d)

DNA Ligase

12.

Which of the following is false about Klenow Fragment

a)

3′→5′3'\rightarrow5' exonuclease activity

b)

5′→3′5'\rightarrow3' exonuclease activity

c)

324-928 residue of polymerase 1

d)

polymerization activity

13.
Which enzyme fixes the Okazaki fragments in the lagging strand?
a)
Helicase
b)
Polymerase
c)
Ligase
d)
Primerase
14.
3' and 5' refer to...
a)
How effectively the helicase works
b)
What direction the nucleotides are pointing
c)
Where the RNA primer attaches
d)
Okazaki's famous experiment
15.
Polymerase is able to replicate the new DNA ______ along the leading strand.
a)
continuously
b)
only in short fragments
c)
very slowly
d)
prokaryotically
16.
order of replication
a)
3, 4, 2, 1
b)
3, 1, 4, 2
c)
2, 1, 4, 3
d)
3, 4, 1, 2
17.
Place the following in sequential order
1. Recoil
2. Straightens the double helix
3. Breaks Hydrogen bonds
4. Adds Free nucleotides
a)
1, 2, 3, 4
b)
3, 4, 2, 1
c)
3, 2, 4, 1
d)
2, 3, 4, 1
18.

Enzyme that connects the DNA to the backbone (glues)

a)

Ligase

b)

Polymerase

c)

Helicase

d)

Primase

19.

#4 in the picture

a)

Polymerase

b)

Primase

c)

Helicase

d)

Ligase

20.

If a person suffered from a disorder where their DNA fragments were unable to fill in the missing slots in between the fragments of the lagging strand, they would most likely have a disorder that affected which enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

21.

Structure C is

a)

helicase

b)

primase

c)

DNA polymerase III

d)

gyrase

22.
The enzyme that removes RNA Primers and fills in the gaps with DNA Nucleotides is
a)
DNA Polymerase III
b)
DNA Polymerase I
c)
DNA Polymerase II
d)
Primase
23.

Which of the following is not a feature of Ori

a)

AT rich sequences

b)

GC rich sequences

c)

Multiple short repeats

d)

none of the above

24.

A replicator Ori C is bound which of the following initiator to form replicator - initiator complex?

a)

DNA B

b)

DNA A box

c)

DNA A

d)

DNA C

25.

Rate of replication in EK is ................than E.coli. select a word from the given options to make correct meaning.

a)

slower

b)

fast

c)

same

26.

Archaebacteria has a MONOREPLICONIC DNA.

a)

TRUE

b)

FALSE

c)

NOT KNOWN

d)

none of the above

27.

Select the TRUE statements from the following. (MORE THAN ONE OPTION MAY BE TRUE)

a)

role of AT rich sequences is to facilitate the melting of DNA

b)

13 mer region is known as DNA A box in E.Coli.

c)

Initiation of replication is regulated by methylation in bacteria

d)

A hemi methylated DNA in bacteria can also initiate replication

e)

For methylation the target sites are GCCT/CTAA

28.

Select the false statements from the following. (MORE THAN ONE OPTION MAY BE FALSE).

a)

In E.coli, the target sequences for methylation are GATC/CTAG

b)

A replicon (ARS) in yeast is larger than E.coli.

c)

A replicon (ARS) in yeast is smaller than E.coli

d)

B domains in ARS are more conserved than A domain in ARS of yeast.

29.

Choose the correct definition of replisome.

a)

Replisome are DNA complexes that carries out replication

b)

replisome is a large protein complexe that carries out DNA replication

c)

replisome is a DNA RNA complex that is formed during replication

d)

replisome is a nucleoprotein

30.

Choose the correct word to fill up the blanks in the following sentence:

REPAIR SYNTHESIS OF DNA REPLACES A ........... STRETCH OF ............. OF DNA WITH A DAMAGED BASE

a)

LONG, ONE STRAND

b)

SHORT, ONE STRAND

c)

LONG, BOTH STRANDS

d)

SHORT, BOTH STRANDS

31.

Which of the following statements are TRUE for DNA polymerase? (More than one options may be true)

a)

DNA synthesizing subunit is the only function of holoenzyme.

b)

DNA synthesizing subunit is one of the several functions of holoenzymes.

c)

DNA polymerase III is a product of gene pol C

d)

DNA polymerase alpha shows priming in EK.

e)

DNA polymerase III is a product of gene pol B

32.

Which of the following statement is true?

a)

Bacterial polymerase enzymes possess the 3'-5' exonuclease activity that proceeds in same direction of DNA synthesis.

b)

Bacterial enzymes possess the 3'-5' exonuclease activity that proceeds in reverse direction of DNA synthesis.

c)

Both of the above

d)

None of the above

33.

Choose the correct sequence of WORDS to answer the following question:

FINGERS, PALM and THUMB of human right hand correspond to which of the following functions?

a)

catalytic site, binds DNA and template positioning.

b)

binds DNA, catalytic site and template positioning

c)

template positioning, binds DNA and catalytic site

d)

template positioning, catalytic site and binds DNA

34.

CORE CATALYTIC ENZYME of DNA Polymerase III in E.coli contains which of the following subunits:

a)

α, e, θ

b)

α, β, θ

c)

α, γ, θ

d)

α, and θ

35.

Which enzyme proofreads and removes RNA Primer during DNA replication.

a)

Ligase

b)

Polymerase 1

c)

Polymerase 3

d)

Helicase

36.
The enzyme that removes RNA Primers and fills in the gaps with DNA Nucleotides is
a)
DNA Polymerase III
b)
DNA Polymerase I
c)
DNA Polymerase II
d)
Primase
37.

Choose the statement that correctly describe the conditions associated with Masselson and Stahl experiment. (more than one option may be correct)

a)

Source of DNA was T4 phage

b)

Source of DNA was E.coli

c)

model organism studied was E.coli

d)

All of the above

38.

In messelson and Stahl's experiment, Nitrogen 'N' was used to incorporate into the newly synthesized DNA. For this, 'N' was supplied in which form?

a)

NH3

b)

NH4Cl

c)

NaNO3

d)

N2

39.

In repeating the Masselson and Stahl Experiment, Three generations are obtained in the laboratory labelled as G0, G1 and G3. Choose the statement that correctly describe G0,/ G1 /G3 in terms of production of band on CsCl Gradient centrifugation.

a)

G0 is the generation from which DNA produces a single N15 band.

b)

G1 refers to DNA obtained after one generation that gives an Intermediate hybrid band.

c)

G2 refers to the generation that produces two types of bands of DNA on CsCl gradient centrifugation.

d)

All of the above

40.

A mode of replication in which two copies of DNA are produced and both contain distinct regions of DNA composed of both original and new strand , is termed as

a)

Conservative mode

b)

Semi-conservative

c)

Dispersive

d)

Non-Dispersive

41.

Which is the last step of DNA replication?

a)

RNA Helicase unwinds original DNA strand

b)

DNA Primase lays down RNA primer for new nucleotide bases.

c)

RNA polymerase builds new strands by bring in new nucleotide bases.

d)

Ligase knits together fragments on lagging strand

42.
Nitrogenous bases are joined by which type of bond?
a)
polar bonds
b)
ionic bonds
c)
covalent bonds
d)
hydrogen bonds
43.

Termination of replication is triggered by-----

a)

Helicase

b)

Topisomerase

c)

SSB

d)

Tus

44.

DNA polymerase III holoenzyme possesses the_____

a)

polymerase activity only

b)

3’→ 5’ endonuclease activity

c)

3’→ 5’ exonuclease activity and polymerase activities

d)

5’→ 3’ exonuclease activity

45.

Which of the following statements are correct about DNA synthesis at the replication fork?

I. Only occurs in 3' to 5' direction

II. Catalyse the elongation of new strand

III. Involve both strands of DNA

a)

I only

b)

I and II only

c)

II and III only

d)

I, II and III

46.

Select the statements that hold TRUE for DNA replication for chromosomes of a rat cell.

a)

replicons located in the same vicinity are to be active at the same time

b)

A pre-replicative complex is formed during S phase prior to replication

c)

At any point of time only some of the replicons are active while others are activated at a specific point of time in S phase

d)

Single strand binding protein is a tetramer

47.

Select the correct options to complete the following statement

ARS in yeast contain consensus sequences,..................................,and ...........................................

a)

B Domain

and

additional elements

b)

A domain

and

additional elements

c)

B1 domain

and

additional elements

d)

None of the above

48.

Select the correct statement that describes DNA polymerase holoenzyme of E.coli.

a)

DNA pol III holoenzyme has at least two catalytic cores, two clamps, and a clamp loader complex

b)

DNA pol III holoenzyme has at least two catalytic cores, a clamp, and a clamp loader complex

c)

DNA pol III holoenzyme has at least three catalytic cores, two clamps, and a clamp loader complex

d)

DNA pol III holoenzyme has only one catalytic core, two clamps, and a clamp loader complex

49.

Choose the correct term to complete the following definition

origin of replication are specific ...........on DNA at which replication begins

a)

sequences

b)

sites

c)

signals

d)

all are correct

50.

Choose the FALSE statements from the following. More than one option may be chosen.

a)

E.coli has single ori.

b)

E.coli has multiple ori

c)

In E.coli, unwinding intiates at 13 mer

d)

13 mer is also known as DNA A box

51.

In Ek, the following protein shows helicase activity

a)

Helicase

b)

MCM protein

c)

geminin

d)

None of the above

52.

Which of the following event is required for transition from G1-S phase in EK.

a)

Methylation of CDC6 and MCM

b)

Phosphorylation of CDC6 and MCM

c)

Dephosphorylation of CDC6 and MCM

d)

Phosphorylation of CDC6 only

53.

In eukaryotic DNA replication, the job of Primer removal is done by which of the following?

a)

RNaseH1

b)

DNA polymerase

c)

Flap endonuclease

d)

RNaseH1+ Flap endonuclease (both)

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