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WorksheetsMicro Final T3 2023
Total questions: 69
Worksheet time: 35mins
Name
Class
Date
1.
Peptidoglycan is found only in the bacterial __________________.
a)
cell membrane
b)
cell wall
c)
endospore
d)
glycocalyx
2.
Which microbe is MOST resistant to being killed?
a)
Gram - bacteria
b)
bacteria which produce capsules
c)
bacteria which produce endospores
d)
bacteria in biofilms
3.
Bacteria having no flagella are unable to
a)
grow in nutrient agar
b)
form biofilms in nutrient agar
c)
move in nutrient agar
d)
reproduce in nutrient agar
4.
Prokaryotes ___________ in that their cells.
a)
have a nucleus
b)
have membrane bound organelles
c)
lack nucleic acids
d)
lack a nucleus
5.
This structure is resistant to heat, cold, drying out, chemicals, antibiotics, and radiation.
a)
Endospores
b)
Axial filaments
c)
Cell membrane
d)
Cell wall
e)
Cytoplasm
6.
The function of this is similar to flagella except they wrap inside the cell.
a)
Axial filaments
b)
Cell membrane
c)
Cell wall
d)
Endospores
7.
Bacterial movement from flagella occurs
a)
from the whipping movement of the flagellum
b)
from the rotation of the flagellum
8.
Flagella allow bacteria to do all of the following EXCEPT
a)
Provide a fluid mosaic model of flexibility.
b)
Sense their environment
c)
Move from a less favorable environment to a more favorable environment
d)
Allows them to exhibit chemotaxis
e)
Allows them to exhibit phototaxis
9.
Which tube has motile bacteria?
a)
The test tube to the left
b)
The test tube to the right
10.
Which letter represents a bacterium with an amphitrichous flagella arrangement?
a)
A
b)
B
c)
C
d)
D
e)
None of the above
11.
Which letter represents a bacterium with an atrichous flagella arrangement?
a)
A
b)
B
c)
C
d)
D
e)
None of the above
12.
Which letter represents a bacterium with monotrichous flagella arrangement?
a)
A
b)
B
c)
C
d)
D
e)
None of the above
13.
Which letter represents a bacterium with peritrichous flagella arrangement?
a)
A
b)
B
c)
C
d)
D
e)
None of the above
14.
This is the most common type of motility for bacteria.
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
15.
Also known as an endoflagellum.
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
16.
This motility spins in a propeller-like motion, either clockwise or counter-clockwise.
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
17.
This type of movement produces a slime and allows the bacteria to spin in a trail of mucus.
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
18.
This moves bacteria by creating a series of runs and tumbles.
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
19.
This is a corkscrew motion and is advantageous for pathogens because it allows the bacteria to burrow through mucus and tissues of the body.
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
20.
This structure can detect chemicals and temperatures outside the cell body and allows for chemotaxis and phototaxis (movement towards/away from chemicals and light).
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
21.
Some bacteria can use their pili to anchor to a surface and pull themselves forward in this type of movement.
a)
Axial filaments
b)
Flagella
c)
Gliding
d)
Twitching
22.
Which Cell type in the picture below is a Gram - cell?
a)
Cell Type A
b)
Cell Type B
23.
Which cell type in the picture below is a Gram + cell?
a)
Cell Type A
b)
Cell Type B
24.
What is the name of structure labeled “A” in the picture below?
a)
Cell wall
b)
Outer membrane
c)
Peptidoglycan
d)
Plasma membrane
e)
Periplasmic Space
25.
What is the name of structure labeled “C” in the picture below?
a)
Cell wall
b)
Outer membrane
c)
Peptidoglycan
d)
Plasma membrane
e)
Periplasmic Space
26.
What is the name of structure labeled “E” in the picture below?
a)
Cell wall
b)
Outer membrane
c)
Peptidoglycan
d)
Plasma membrane
e)
Periplasmic Space
27.
What is the name of structure labeled “F” in the picture below?
a)
Cell wall
b)
Outer membrane
c)
Peptidoglycan
d)
Plasma membrane
e)
Periplasmic Space
28.
What are active bacteria that grow, metabolize, and reproduce called?
a)
Dormant cells
b)
Endospores
c)
Germinating cells
d)
Vegetative cells
29.
What are cells called when endospores revert back to a vegetative cell called?
a)
Dormant cells
b)
Endospores
c)
Germinating cells
d)
Vegetative cells
30.
What is inside of an endospore? (Mark all that apply)
a)
Cytoplasm
b)
DNA
c)
Enzymes
d)
Ribosomes
31.
True or False: Endospore formation in bacteria is a reproductive process.
a)
True
b)
False
32.
What is the primary stain for the endospore stain?
a)
Malachite Green
b)
Alcohol
c)
Crystal Violet
d)
Heat
e)
Iodine
33.
What is the mordant for the endospore stain?
a)
Heat
b)
Alcohol
c)
Crystal Violet
d)
Iodine
e)
Malachite Green
34.
What is the decolorizer for the endospore stain?
a)
Water
b)
Alcohol
c)
Crystal Violet
d)
Heat
e)
Iodine
35.
What is the counter stain for the endospore stain?
a)
Safranin
b)
Alcohol
c)
Crystal Violet
d)
Heat
e)
Iodine
36.
What color will the endospores be?
a)
Green
b)
Pink
c)
Purple
37.
What color will the vegetative cells be?
a)
Green
b)
Pink
c)
Purple
38.
Which cell (s) contain an ellipsoid spore shape?
a)
a
b)
b
c)
c
39.
Which cell (s) contain a terminal spore location?
a)
a
b)
b
c)
c
40.
Which cell (s) contain a subterminal spore location?
a)
a
b)
b
c)
c
41.
A Gram stain is usually the first test performed for the identification of bacteria.
a)
True
b)
False
42.
Which cell type will stain purple under the microscope.
a)
Gram positive cells
b)
Gram negative cells
43.
Which cell type will stain pink under the microscope.
a)
Gram positive cells
b)
Gram negative cells
44.
Which cell type have small amounts of peptidoglycan in their cell walls?
a)
Gram positive cells
b)
Gram negative cells
45.
Which cell type have large amounts of peptidoglycan in their cell walls?
a)
Gram positive cells
b)
Gram negative cells
46.
Heat fixing is an important step in Gram Staining. Why? (choose all that apply)
a)
It ensures that the bacteria dry on the slide
b)
It prevents the bacteria from rinsing off when using the chemicals during Gram Staining
c)
It kills the bacteria
d)
It destroys the toxins
e)
It allows the cell envelope to be more permeable to the staining process
47.
What is the correct order of reagents in the Gram Stain lab?
a)
Crystal violet, Alcohol, Iodine, Safranin
b)
Crystal violet, Iodine, Safranin, Alcohol
c)
Crystal violet, Iodine, Alcohol, Safranin,
d)
Crystal Violet, Safranin, Iodine, Alcohol
48.
The bacteria in the illustration below would most likely be which bacteria from our lab?
a)
Escherichia coli
b)
Bacillus megaterium
c)
Lactococcus lactis
d)
Micrococcus luteus
e)
Sarcina aurantiaca
49.
The bacteria in the illustration below would most likely be which bacteria from our lab?
a)
Sarcina aurantiaca
b)
Bacillus megaterium
c)
Escherichia coli
d)
Lactococcus lactis
e)
Micrococcus luteus
50.
The bacteria in the illustration below would most likely be which bacteria from our lab?
a)
Lactococcus lactis
b)
Bacillus megaterium
c)
Escherichia coli
d)
Micrococcus luteus
e)
Sarcina aurantiaca
51.
Which arrangement listed below does not exist in bacteria?
a)
Staphylobacillus
b)
Diplococcus
c)
Diplobacillus
d)
Staphylococcus
e)
Streptococcus
52.
A selective media
a)
Contains compounds that inhibit the growth of certain bacteria
b)
Contains compounds that allow for visual distinction of the ability to perform a specific biochemical process
c)
Must contain bile salts, crystal violet, and red blood cells
d)
Can never be a differential media
53.
A differential media
a)
Contains compounds that inhibit the growth of certain bacteria
b)
Contains compounds that allow for visual distinction of the ability to perform a specific biochemical process
c)
Must contain bile salts, crystal violet, and red blood cells
d)
Can never be a differential media
54.
The differential aspect of a selective growth medium reveals what about the bacteria growing there?
a)
the bacterial cell wall structure
b)
what it can metabolize (eat / break down)
c)
if it is Gram+ or Gram-
d)
if it can grow in a salty environment
e)
its cell morphology
55.
MacConkey's agar will only grow bacteria that are
a)
Acid fast
b)
Gram +
c)
Gram -
d)
Halophilic
56.
If Mannitol Salt agar is growing bacteria, and the medium itself appears bright yellow, which species of bacteria is growing?
a)
Salmonella pullorum
b)
Staphylococcus epidermidis
c)
Staphylococcus aureus
d)
Escherichia coli
e)
Streptococcus pyogenes
57.
If Mannitol Salt agar is growing bacteria, and the medium has not changed color and remains red, which species of bacteria is growing?
a)
Salmonella pullorum
b)
Staphylococcus epidermidis
c)
Staphylococcus aureus
d)
Escherichia coli
e)
Streptococcus pyogenes
58.
EMB agar will only grow bacteria that are
a)
Acid fast
b)
Gram +
c)
Gram -
d)
Halophilic
59.
MSA agar will only grow bacteria that are
a)
Acid fast
b)
Gram +
c)
Gram -
d)
Halophilic
60.
Is blood agar selective, differential, both or neither?
a)
selective
b)
differential
c)
both
d)
neither
61.
Is MSA agar selective, differential, both or neither?
a)
selective
b)
differential
c)
both
d)
neither
62.
Is MAC agar selective, differential, both or neither?
a)
selective
b)
differential
c)
both
d)
neither
63.
Is EMB agar selective, differential, both or neither?
a)
selective
b)
differential
c)
both
d)
neither
64.
What is the name of the agar in the picture below?
a)
Blood Agar
b)
Eosin methylene blue agar (EMB)
c)
MacConkey agar (MAC)
d)
Mannitol Salt agar (MSA)
65.
What is the name of the agar in the picture below?
a)
Blood Agar
b)
Eosin methylene blue agar (EMB)
c)
MacConkey agar (MAC)
d)
Mannitol Salt agar (MSA)
66.
What is the name of the agar in the picture below?
a)
Blood Agar
b)
Eosin methylene blue agar (EMB)
c)
MacConkey agar (MAC)
d)
Mannitol Salt agar (MSA)
67.
What is the name of the agar in the picture below?
a)
Blood Agar
b)
Eosin methylene blue agar (EMB)
c)
MacConkey agar (MAC)
d)
Mannitol Salt agar (MSA)
68.
Which illustration below represents a bacterium with gamma hemolysis?
a)
A
b)
B
c)
C
d)
None of the above
69.
Which illustration below represents a bacterium with delta hemolysis?
a)
A
b)
B
c)
C
d)
None of the above
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