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Topic 1 Review

Total questions: 76

Worksheet time: 42mins

Name
Class
Date
1.

The process of DNA Fingerprinting is based on the fact that

a)

everyone has the exact same DNA.

b)

no 2 people, except identical twins, have exactly the same DNA as someone else.

c)

most genes are dominant.

d)

None of the other answer choices are correct.

2.

(GAAT) (GAAT) (GAAT) (GAAT) is an example of:

a)

restriction fragments.

b)

DNA fingerprinting.

c)

PCR.

d)

STR.

3.

The total amount of DNA in a cell, which is contained in the cell's nucleus (nuclear DNA) and mitochondria (mtDNA), is called the human:

a)

genome.

b)

gene.

c)

allele.

d)

RNA.

4.

Which method is used to identify different STR markers?

a)

x-raying DNA samples

b)

extracting the mtDNA from the gene

c)

adding fluorescent dyes to the PCR reaction

d)

calculating the allele frequency

5.

What material is used to isolate and cut DNA?

a)

separating enzymes

b)

restriction enzymes

c)

PCR

d)

base pairs

6.

Name a method of separating molecules, such as DNA, based on their size

a)

electrophoresis

b)

restriction enzymes

c)

STR

d)

PCR

7.

Amplifying trace samples of DNA is called

a)

introns

b)

restriction enzymes

c)

STR

d)

PCR

8.

If guanine makes up 19% of the nucleotides in a sample of DNA from an organism, then cytosine would make up _____ percent of the bases.

a)

19%

b)

62%

c)

31%

d)

38%

9.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
10.
Which two bear species are most closely related?
a)
Bear 1 and Bear 2
b)
Bear 1 and Bear 3
c)
Bear 2 and Bear 3
d)
There’s no way to tell
11.
Which man is the father of this baby?
a)
Father 1
b)
Father 2
c)
Father 3
d)
None of them
12.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
a)
DNA is negatively charged so attracted to the positive end of the unit
b)
DNA is positively charged to attracted to the negative end of the unit
c)
the agarose gel in negatively charged
d)
the agarose gel is positively charged
13.

What is the purpose of PCR?

a)

To produce millions of copies of the DNA genome

b)

To produce millions of copies of a specific region of DNA

c)

To add nucleotides to a DNA sequence

d)

To watch polymerase work.

14.

What happens in the denature step of PCR?

a)

The DNA nucleotides are broken apart.

b)

The base-pairing rules for DNA are reversed.

c)

The double-stranded DNA is separated into two single strands of DNA.

d)

The DNA is returned to its natural setting.

15.

At what temperature does the denature step of PCR occur?

a)

94-96° C

b)

50-65° C

c)

72° C

d)

Any temperature

16.

At what temperature does the extension step of PCR occur?

a)

94-96°C

b)

50-65°C

c)

72°C

d)

Any temperature

17.

What happens during the anneal step of PCR?

a)

Primers are created.

b)

The primers attach to the target DNA region.

c)

Primers copy the new DNA strand.

d)

Primers sequence DNA

18.

What is the function of a primer?

a)

To identify the particular region of DNA to be copied by PCR.

b)

To copy DNA.

c)

To create DNA nucleotides.

d)

To maintain the temperature of the PCR reaction.

19.

From where does Taq polymerase come?

a)

Bacteria that live in hydrothermal vents/hot springs.

b)

Pig cells

c)

The rain forest

d)

A Day Spa

20.

DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.

a)

positive, negative

b)

positive, positive

c)

negative, positive

d)

negative, negative

21.

Larger DNA fragments move through a gel _____________ smaller DNA fragments.

a)

faster than

b)

at the same rate as

c)

slower than

22.

Which two DNA fragments are the same size?

23.

Which lane of the gel has a DNA fragment that is about 700 base pairs?

a)

2

b)

3

c)

4

d)

5

24.

All cells carry the entire genome of an organism apart from:

a)

hair follicles and gametes.

b)

skin cells and gametes.

c)

gametes and red blood cells.

d)

gametes.

25.

Gene expression is the ability for a gene to be:

a)

in the genome.

b)

transcribed.

c)

non-coding.

d)

a dominant allele.

26.

Which RNA type is the result of transcription?

a)

tRNA

b)

Non-coding RNA

c)

rRNA

d)

mRNA

27.

Which of the following is correct?

a)

Nucleotide triplets code a single amino acid.

b)

Each amino acid codes a nucleotide.

c)

Each nucleotide codes an amino acid.

d)

Amino acid triplets code a single nucleotide.

28.

RNA substitutes uracil for which nucleotide?

a)

Adenine

b)

Cytosine

c)

Guanine

d)

Thymine

29.

Epigenetic factors affect phenotype because...

a)

they can up-regulate gene expression

b)

high temperature causes us to sweat and turn red, which changes our appearance

c)

certain nucleotides in the mRNA sequence get methylated

d)

some nucleotides in the DNA sequence have a methyl group added to them

30.

Translation is a process where:

a)

a polypeptide sequence is produced from a sequence of rRNA codons.

b)

a polypeptide sequence is produced from a sequence of tRNA codons.

c)

a polypeptide sequence is produced from a sequence of mRNA codons.

d)

a DNA nucleotide sequence is used to produce mRNA codon sequence.

31.

Epigenetics modulates genetic expression:

a)

differently in each cell in the body.

b)

by altering the genome.

c)

in response to only intracellular processes.

d)

consistently throughout an individual's life.

32.

Transcription factors are produced by:

a)

coding DNA.

b)

structural genes.

c)

non-coding DNA and structural genes.

d)

non-coding DNA.

33.

Hox genes are the most powerful control on the morphology of animals. They do this by:

a)

being highly conserved.

b)

coding for the body plan.

c)

coding body segmentation.

d)

regulating transcription of other genes.

34.

Monozygotic twins can look different to one another because:

a)

they have different genetic material from the mother but the same genetic material from the father.

b)

they have different genetic material from the father but the same genetic material from the mother.

c)

the environment is determinative of phenotype.

d)

a mutation has occurred in one twin during embryonic development.

35.

A traumatic event experienced by an organism can affect the health of its children and grandchildren. This can happen through inherited:

a)

epigenetic tags (methylation patterns).

b)

polypetide chains.

c)

transcription factors.

d)

DNA nucleotide patterns.

36.

A split gene includes non-coding introns that are ____________ during post-transcriptional modification.

a)

capped

b)

removed

c)

retained

d)

methylated

37.

DNA methylation patterns are generally:

a)

predictable

b)

substantially altered when an individual smokes.

c)

inherited from the parents.

d)

set early in embryonic development.

38.

The homeobox gene is highly conserved. This shows that:

a)

it is a sequence that is unlikely to mutate.

b)

its role in gene expression is vital to survival.

c)

it is coded for in several places.

d)

it is very powerful; therefore maintained by evolution.

39.

Gene regulation occurs:

a)

only in some cells.

b)

when the variability within the genetic code is insufficiently adaptive for the individual to survive in its environment.

c)

constantly, in all known life forms.

d)

during embryonic development.

40.
_______ is to transcription as ________ is to translation.
a)
mRNA / Amino Acid Chain
b)
Amino Acid Chain / mRNA
c)
Ribosome / Nucleus
d)
mRNA / tRNA
41.
Which of these processes begin with an mRNA molecule?
a)
DNA Replication
b)
Transcription
c)
Translation
d)
None of them
42.

Translation in eukaryotes occurs:

a)

outside of the nucleus.

b)

inside of the nucleus.

43.
What structure of this molecule binds with the mRNA molecule?
a)
Amino Acid
b)
tRNA
c)
Anticodon
d)
Codon
44.

What is the monomer of DNA?

a)

amino acid

b)

monosaccharide

c)

nucleotide

d)

fatty acid and glycerol

45.

What are the three parts of a nucleotide?

a)

phosphate group

b)

nitrogen base

c)

sugar

d)

fatty acid

46.

This is what part of the nucleotide?

a)

phosphate group

b)

nitrogen base

c)

sugar

d)

fatty acid

47.

This is what part of the nucleotide?

a)

phosphate group

b)

nitrogen base

c)

sugar

d)

fatty acid

48.

This is what part of the nucleotide?

a)

phosphate group

b)

nitrogen base

c)

sugar

d)

fatty acid

49.

What sugar is found in a nucleotide of DNA?

a)

deoxyribose

b)

ribose

c)

trioxyribose

d)

oxyribose

50.

The twisted ladder shape of DNA is called a ______________?

a)

twisted noddle

b)

winding helix

c)

double helix

d)

triple helix

51.

Which nitrogenous base or bases correctly matches up with Cytosine?

a)

adenine

b)

thymine

c)

guanine

d)

uracil

52.

Which nitrogenous base or bases correctly matches up with guanine?

a)

adenine

b)

thymine

c)

cytosine

d)

uracil

53.

Which nitrogenous base or bases correctly matches up with adenine?

a)

guanine

b)

thymine

c)

cytosine

d)

uracil

54.

Which nitrogenous base or bases correctly matches up with thymine?

a)

guanine

b)

adanine

c)

cytosine

d)

uracil

55.

Two hydrogen bonds hold together which two hydrogen bases?

a)

A & T

b)

G & C

c)

A & C

d)

T & G

56.

Three hydrogen bonds hold together which two hydrogen bases?

a)

A & T

b)

G & C

c)

A & U

d)

T & G

57.

Adenine and Thymine are said to be ____________ base-pairs.

a)

matching

b)

complementary

c)

incongruous

d)

discordant

58.

What is the correct matching sequence to the following DNA Strand?

ATTCGCGA

a)

TAAGCGCT

b)

TAAGAGCT

c)

TAAGCGCA

d)

CAAGCGCT

59.
What is a protein: 
a)
A polymer composed of many sugars.
b)
A polymer composed of many amino acids. 
c)
A polymer composed of fatty acids. 
d)
A polymer composed of nucleotides. 
60.
How many different amino acids are there?
a)
15
b)
20
c)
25
d)
30
61.
This is one job proteins do NOT have in the body
a)
storing genetic information
b)
structure
c)
speed up chemical reactions
d)
transport things through cell membrane
62.
What is the monomer of proteins?
a)
amino acids
b)
polypeptides
c)
nucleotides
d)
monosaccharides
63.
The diagram shows the structure of an amino acid. Which type of molecule is formed from amino acids?
a)
lipids
b)
proteins
c)
carbohydrates
d)
nucleic acids
64.
The diagram shows a bond forming between two amino acids. What is the name of this reaction?
a)
Dehydration Synthesis
b)
Hydrolysis
c)
Pepysis
d)
Oxidation
65.
Each amino acid is different from the other due to....
a)
The R group
b)
The amino group
c)
The carboxyl group
d)
The central carbon
66.

What is a long chain of amino acids called?

a)

Polysaccharide

b)

Lipid

c)

Carbohydrate

d)

Polypeptide

e)

Nucleic acid

67.

What is the name of the bond that joins two amino acids together?

a)

Ester bond

b)

Glycosidic bond

c)

Peptide bond

d)

Nucleic bond

68.

All proteins

a)

have a unique structure that determines their unique function.

b)

are exactly the same in function but different in structure.

c)

are exactly the same in structure but different in function.

d)

are enzymes.

69.

Proteins are made of

a)

CHO

b)

CHON

c)

CHONP`

d)

CHONS

70.

This is one job proteins do NOT have in the body

a)

insulation

b)

structure for tissues and organs

c)

speeds up chemical reactions

d)

transport things through cell membrane

71.
Amino acids are a type of water soluble molecule.  How do they pass through the cell membrane?
a)
Diffuse across the membrane
b)
Enter via a vesicle
c)
Pass between the small pores between the phospholipid molecules
d)
Transferred by carrier proteins
72.
The production of proteins occurs in the
a)
endoplasmic reticulum
b)
mitochondria
c)
ribosomes.
d)
nucleus.
73.
The cell organelle that packages proteins inside the cell before they are sent to their destination is the 
a)
golgi apparatus
b)
centriole
c)
smooth endoplasmic reticulum
d)
ribosome
74.
Ridged outer layer of a plant cell.
a)
Cell Membrane
b)
Cell Wall
c)
ER
d)
Ribosomes
75.
Stores food, water, wastes, and other materials.
a)
Vacuoles
b)
Mitochondria
c)
Chloroplasts
d)
Golgi Bodies
76.
The cell structure that converts the sun's energy into food (sugar) is the?
a)
mitochondrion
b)
chloroplast
c)
cell wall
d)
cell membrane