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Biol111 DNA replication

Total questions: 53

Worksheet time: 53mins

Name
Class
Date
1.

Which of the following units are repeatedly joined together to form a strand of DNA?

a)

Amino Acids

b)

Nucleotides

c)

Fatty Acids

d)

Polysaccharides

2.

Nucleotides polymerize by adding from

a)

3’ to 5’

b)

5’ to 5’

c)

5’ to 3’

d)

3’ to 3’

3.

The process of DNA replication is called semi conservative because it results in

a)

none of these

b)

Two DNA molecules.  One original molecule and one with two new strands

c)

two exact copies with new strands

d)

two DNA molecules, each with 1 original strand and one new.

4.

If the structure of DNA was not anti parallel, then it would not have a double helix

a)

True

b)

False

5.

Nitrogen bases are joined by which type of bond?

a)

polar bonds

b)

hydrogen bonds

c)

phosphate bonds

d)

deoxyribose bonds

6.

Adenine bonds with _____

a)

Cytosine

b)

Thymine

c)

Guanine

d)

Uracil

7.

Cytosine bonds with ____

a)

Adenine

b)

Thymine

c)

Guanine

d)

Uracil

8.

What does DNA stand for?

a)

Deoxyribonucleic acid

b)

Denitrogenous acid

c)

Deribonucleic acid

d)

Diribonucleic acid

9.

The picture below shows the steps of DNA replication beginning with step one and ending with step three.


​Because you are reusing the old, or parent, DNA strand DNA replication is said to be

a)

Semi- Conservative

b)

Semi-discontinuous

c)

Continuous

d)

Fully-Conserveative

10.

The picture below shows an enzyme unzipping DNA.

​

​What is the name of this enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

DNA topoisomerase

11.

Match the nucleotide with its complementary nucleotide.


Cytosine-

a)

Thymine

b)

Guanine

c)

Adenine

d)

Cytosine

12.

Which enzyme is responsible for adding the free nucleotides to the replicating DNA? ​

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase 3

d)

DNA polymerase 1

13.

Which is the first step of DNA replication?

a)

Topoisomerase unwinds original DNA strand

b)

RNA Primase lays down RNA primer for new nucleotide bases.

c)

DNA polymerase builds new strands by bring in new nucleotide bases.

d)

Helicase separates the DNA strand

14.

Which is the second step of DNA replication?

a)

DNA Helicase unzips original DNA strand

b)

RNA Primase lays down RNA primer for new nucleotide bases.

c)

DNA polymerase builds new strands by bring in new nucleotide bases.

d)

Ligase knits together fragments on lagging strand

15.

Which is the third step of DNA replication?

a)

DNA Helicase unwinds original DNA strand

b)

Primase lays down RNA primer for new nucleotide bases.

c)

DNA polymerase builds new strands by bring in new nucleotide bases.

d)

Ligase knits together fragments on lagging strand

16.

Which is the fourth step of DNA replication?

a)

DNA Helicase unwinds original DNA strand

b)

Primase lays down RNA primer for new nucleotide bases.

c)

DNA polymerase builds new strands by bring in new nucleotide bases.

d)

Ligase knits together fragments on lagging strand

17.

What is the fifth step in dna replication?

a)

Helicase unzips nucleotides

b)

Topoisomerase unwinds DNA

c)

Ligase glues nicks together

d)

DNA polymerase 3 adds nucleotides

18.

Which letter in the picture below depicts the leading strand in DNA replication?

a)

A

b)

C

c)

B

d)

Both A and B

19.

Which letter in the picture below depicts the lagging strand in DNA replication?

a)

A

b)

C

c)

B

d)

Both A and B

20.

If a person suffered from a disorder where their DNA fragments were unable to fill in the missing slots in between the fragments of the lagging strand, they would most likely have a disorder that affected which enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

21.

Okazaki fragments appear on which strand of DNA during DNA replication?

a)

lagging

b)

leading

22.
Which 2 molecules forms the sides (backbone) of the DNA ladder?
a)

deoxyribose and nitrogenous base

b)
deoxyribose and a hydrogen bond
c)

Nitrogenous base and phosphate

d)

deoxyribose sugar and phosphate

23.
Which sequence of DNA bases would pair with this partial strand
ATG TGA CAG
a)
ATG TGA CAG
b)
TAC ACT GTC
c)
GTA AGT GAC
d)
CAT TCA CTG
24.
Name this structure
a)
double helix
b)
nucelotide
c)

Nitrogenous base

d)
hydrogen bond
25.

What is the difference between a nitrogenous base and a nucleotide?

a)

A nitrogenous base is a part of a nucleotide; a nucleotide includes a nitrogenous base, sugar, and phosphate group.

b)

a nucleotide is a part of a nitrogenous base; a nitrogenous base includes a nucleotide, sugar, and phosphate group

c)
Nitrogenous bases are only found in RNA.
d)
A nucleotide is a type of nitrogenous base.
26.
A nucleotide includes...?
a)
pentose sugar
b)
nitrogenous base
c)
phosphate group
d)
all of the above
27.
In a molecule of double-stranded DNA, the amount of Adenine present is always equal to the amount of
a)
cytosine
b)
guanine
c)
thymine
d)
uracil
28.
Which organelle is DNA usually found in?
a)
cell membrane
b)
vacuole
c)
chloroplast
d)
nucleus
29.
order of replication
a)
3, 2, 1, 4
b)
2, 1, 4, 3
c)
2, 3, 1, 4
d)
3, 4, 1, 2
30.

Enzyme that fills in the gaps between nucleotides

a)

Ligase

b)

Polymerase

c)

Helicase

d)

Primase

31.

Enzyme that breaks hydrogen bonds (unzips)

a)

Ligase

b)

Polymerase

c)

Primase

d)

Helicase

32.

Site where replication begins

a)

Polymerase action site

b)

Replication fork

c)

Replication Origin

d)

In the front

33.

The new DNA strand formed after DNA replication is an exact copy of its parent strand

a)

True

b)

False

34.

Proteins that DNA winds itself around to create a Chromosome

a)

Histones

b)

Enzymes

c)

Chromatin

d)

DNA polymerase

35.
DNA codes for...
a)
cholesterol
b)
proteins
c)
fatty acids
d)
a sandwich
36.
Before the cell divides, what process needs to happen?
a)
Transcription
b)
Translation
c)
Replication
d)
Protein synthesis
37.

Which bases are purines?

a)

A

b)

G

c)

T

d)

C

38.

Which bases are pyramidines?

a)

C

b)

T

c)

G

d)

A

39.

The percentage of A and T are equal, and C and G are equal

a)

True

b)

False

40.

What is the purpose of SSBs?

a)

Snipping DNA

b)

Removes tension in strand

c)

Prevents the strands from making secondary structures (Bonding back together)

d)

Separates the bases

41.

What does topoisomerase do?

a)

It relives tension in the strand By unwinding

b)

It separates hydrogen bonds

c)

It glues the fragments together

d)

It prevents secondary structures from forming

42.

A template strand can be read either 3’ to 5’ or 5’ to 3’

a)

False, a template strand can only be read 5' to 3'.

b)

True, a template strand can be read in both directions simultaneously.

c)

False, a template strand cannot be read at all.

d)

False, a template strand can only be read 3' to 5'.

43.

A segment of DNA is shown in the diagram. Which of these identifies the part of the DNA segment labeled X?

a)

Nucleotide

b)

Phosphate group

c)

Hydrogen bond

d)

Deoxyribose

44.

What is a phosphodiester bond between?

a)
The 5' hydroxyl group of one nucleotide and the 3' phosphate group of another nucleotide.
b)
The 3' hydroxyl group of one nucleotide and the 5' phosphate group of another nucleotide.
c)
The 3' phosphate group of one nucleotide and the 5' hydroxyl group of another nucleotide.
d)
The 2' hydroxyl group of one nucleotide and the 4' phosphate group of another nucleotide.
45.

What way does a replication bubble open?

a)

<— and —>

b)

—> only

c)

<— only

d)

—> and <—

46.

What is an origin of replication?

a)
A specific sequence in DNA where replication begins.
b)
A region in RNA where transcription starts.
c)
A part of the cell where proteins are synthesized.
d)
A sequence in DNA that codes for a specific protein.
47.

What determines the leading strand vs the lagging strand?

a)
The type of nucleotides present in the DNA strand.
b)
The presence of RNA primers in the strand.
c)
The overall length of the DNA molecule.
d)
The direction of DNA synthesis relative to the replication fork.
48.

What is the correct way to label the carbons on a sugar?

a)

b)

c)

d)

49.

How many origins of replication can there be?

a)

Multiple origins of replication in eukaryotes

b)

No origins of replication in eukaryotes

c)

One origin of replication in eukaryotes

50.

What is the difference between DNA and RNA?

a)
DNA contains uracil; RNA contains thymine.
b)
DNA is single-stranded and contains ribose; RNA is double-stranded and contains deoxyribose.
c)
DNA is double-stranded and contains deoxyribose; RNA is single-stranded and contains ribose.
d)
DNA is found only in the nucleus; RNA is found only in the cytoplasm.
51.

What is the difference between a replication fork and a replication bubble?

a)

A replication fork is the point of separation of DNA strands during replication that occurs in one direction, while a replication bubble is a region where replication occurs in both directions, containing two forks.

b)
A replication fork contains two bubbles of DNA strands.
c)
A replication bubble is the point of separation of DNA strands during replication.
d)

A replication bubble is the point of separation of DNA strands during replication that occurs in one direction, while a replication fork is a region where replication occurs in both directions, containing two bubbles.

52.

Where is a nucleotide added to another nucleotide?

a)

At either the 3' end or the 5’ end

b)

At the 3' end

c)

At the 5' end

d)

In between the phosphodiester bond

53.

What is the correct order of enzymes used in DNA replication?

a)

Topoisomerase, Helicase, Primase, DNA Polymerase, Ligase

b)

DNA Polymerase, Helicase, Ligase, Primase, topoisomerase

c)

Primase, Ligase, Helicase, topoisomerase, DNA Polymerase

d)

Topoisomerase, DNA Ligase, Primase, Helicase, DNA Polymerase

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