WorksheetsBasics of Microbiology- Common staining 1 & 2
Total questions: 46
Worksheet time: 23mins
What is the purpose of fixation and staining for microscopic examination of microorganisms?
increase visibility
highlight specific morphological feature
preservation
All of the above
What are the benefits of staining microorganisms?
increase visibility
highlight specific morphological features
preserves them for future use
all of the above
It is important to stain but not fix microorganisms before viewing them under a microscope?
true
false
What are the steps for the preparation of a stained bacterial blood smear?
slide preparation---smear preparation---fixation---staining---labelling
smear preparation---labelling---slide preparation---fixation---staining
slide preparation---labelling---smear preparation---fixation---staining
smear preparation---fixation---staining---slide preparation---labelling
What is a thin layer of bacteria placed on a slide for staining called?
bacterial inoculum
bacterial smear
bacterial fixation
labelling
Which of the following is not true about the slide preparation before staining?
grease or oil free slides are necessary
slides need not to be dried
slides should be washed with soap and water
slides should be wiped with spirit or alcohol
What is the name of the instrument used to make bacteria smear?
micropipette
inoculating loop
slide edge
coverslip
What is the motion used to make bacterial smear on slide?
motion of 8
circular motion
unidirectional
randomly
Which of the following statement is true?
the loop should always be sterilized (heating or red hot) before making the bacterial smear
this is done to prevent contamination
both are right
both are wrong
Which of the following statement is incorrect regarding heat fixation of bacterial smear?
routinely used to observe prokaryotes
done by passing the smear over flame two to three times with the smear side up
preserves over all morphology
it preserves the structures within cell
Smear fixation is done to inactivate enzymes that disrupt cell structures.
true
false
Which of the following is used to enhance contrast in the microscopic image?
slide preparation
smear preparation
staining
inoculation
How does the staining colour to cells?
stain adheres to slide
stain adheres to cell
stain adheres to microscope
none
Which of the following is not a simple stain?
gram stain
methylene blue
dilute carbon fuchsin
polychrome methylene blue
Which of the following is true about the following statement?
A chemical compound that reacts with the stain to form an insoluble , colored precipitate.
primary stain
secondary stain
mordant
decolorizer
What is the correct phrase for the following?
One step staining method using only one dye.
simple staining
differential staining
special stain
all of these
Which of the following statement is true?
1.Polychrome methylene blue is a special stain.
2.It is made up after ripening of Loeffler's methylene blue.
1
2
1&2
None
During preparation of carbol fuchsin , it is diluted to ---- by sing distilled water.
1/5
1/10
1/15
1/20
How much time does the ripening of Loeffler's methylene blue takes?
9 months
10 months
12 months
15 months
Which of the following is the correct order of staining using carbol fuschsin?
flood the smear/ wash with tap water after 30 sec./ gently blot dry
wash with tap water after 30 sec/ gently blot dry/flood the smear
gently blot dry/flood the smear/ wash with tap water after 30 sec
gently blot dry/ wash with tap water after 30 sec/ flood the smear
What is the purpose of differential staining?
to provide inflammation about flagella
to view the motility of bacteria
to allow the cells to be categorized into various groups or types
to view the nucleus better
Which of the following is among the differential stains?
gram stain
ziehl Neelsen stain
Both A & B
India ink stain
After gram staining, which colour will gram positive bacteria appear?
violet colour
pink
orange
yellow
Gram staining is done based in differences in chemical and physical properties of the cell wall?
true
false
Which of the following is the first step in the identification of a bacterial organism?
acid fast staining
gram staining
Giemsa staining
None
On What basis does the Gram Staining categorize bacteria into gram positive and gram negative bacteria?
absence or presence of flagella
differences in cell wall
pathogenecity
virulence characteristics
Which is the most important and widely used staining technique in bacteriology?
Romansky staining
pap staining
gram staining
Ziehl Neelsen staining
What is the use of iodine in gram staining?
fixative
mordant
solublizer
stain
What are the most common stain used in Gram staining?
crystal violet and methylene blue
crystal violet and safranin
crystal violet and carbol fuchsin
methylene blue and safranin
Which of the following is among the counterstain used in Gram staining?
crystal violet
methylene blue
safranin
carbol fuschin
Which of the following is the decolorizer used in Gram staining?
potassium iodide
safranin
95% ethanol
ammonium oxalate
What is the correct order of staining reagents in Gram staining?
crystal violet,alcohol, iodine solution, safranin
crystal violet, iodine solution, alcohol, safranin
crystal violet, safranin, alcohol, iodine solution
iodine solution,crystal violet, alcohol, safranin
What happens with the gram negative bacteria after ethanol treatment in Gram staining?
decolorisation
resist decolorisation
color intesifies
none
What color do gram positive and gram negative bacteria take respectively?
pink & purple
purple & pink
orange & yellow
yellow & orange
Which of the following is gram negative bacteria?
staphylococcus
streptococci pyogenes
salmonella
clostridium
Which of the following is a gram positive bacteria?
Escherichia coli
Salmonella typhi
Clostridia
Vibrio cholera
After gram staining , organisms that appear pink are ---- in nature?
gram positive
gram negative
none
both A& B
Which gram negative bacteria causes whooping cough?
Salmonella typhi
Bordetella pertusis
Vibrio cholera
Escherichia coli
What is the other name for acid fast stain?
Ziehl Neelsen stain
capsular stain
gram staining
Romansky staining
Which of the following used acid fast staining for its diagnosis?
Typhoid
Tuberculosis
Tetanus
All of the above
Which of the following is the primary stain used in the acid fast stain procedure?
acid alcohol
carbol fuchsin
Methylene blue
Malachite green
What percentage of acid alcohol is used as a reagent in case of acid fast staining?
1%
2%
3%
4%
What is the correct order of staining reagents in acid fast staining?
carbol fuschin----mordant---acid alcohol---methylene blue
methylene blue---mordant---acid alcohol---carbol fuschin
acid alcohol---methylene blue---carbol fuschin---mordant
acid alcohol---methylene blue---morgant---carbol fuschin
Which of the following is used to decolorize the carbol fuchsin in the acid fast stain procedure?
methylene blue
malachite green
distilled water
acid alcohol
What is the role of methylene blue in acid fast staining?
mordant
counterstain
primary stain
decoloriser
What is the colour of the following group of bacteria on acid fast staining?
1.AFB
2.Other
Red, blue
blue,red
red,red
red,colourless
