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BIO 121 Lab Exam 1

Total questions: 69

Worksheet time: 37mins

Name
Class
Date
1.

To what number would you pull your solution to transfer 2ml in this pipette?

a)

4

b)

3

c)

2

d)

1

2.

What is the solution called that is used to calibrate a spectrophotometer?

a)

Substrate

b)

Solvent blank

c)

Solute

d)

Solution

3.

Which letter represents the button you would push to calibrate a spectrophotometer?

a)

A

b)

E

c)

C

d)

D

4.

Given that a serial dilution was performed as shown in the picture and the concentration of the stock solution is 50mM, what is the concentration of the 3rd test tube?

a)

100

b)

25

c)

12.5

d)

6.25

5.

A solution consists of a (a)   dissolved into a solvent.

6.

Transmittance is a linear function of concentration.

a)

True

b)

False

c)

Only on Wednesdays

d)

Absorbance

7.

If the stock solution is test tube #1, and the test tube at the end on the right is #6, which test tube would have the greatest reading for %Transmittance after the serial dilution was completed?

a)

1

b)

2

c)

5

d)

6

8.

If test tube #1 is the stock solution and the test tube at the end on the right is #6, which test tube would have the highest Absorbance reading after the completion of the serial dilution?

a)

1

b)

2

c)

5

d)

6

9.

As the value for %T increases, what happens the value of absorbance?

a)

stays the same

b)

increases

c)

decreases

10.

What happens to %Transmittance readings as concentration increases?

a)

increase

b)

decrease

c)

stay the same

11.

What happens to Absorbance readings as concentration increases?

a)

increases

b)

decreases

c)

no effect

12.

If this graph depicts the relationship of %Transmittance and concentration, which value goes on the X-axis?

a)

%transmismittance

b)

absorbance

c)

concentration

13.

Given the following graph, what is the concentration of an unknown solution given a %Transmittance reading of 30?

a)

80mM

b)

80

c)

26

d)

65mM

14.

The graph depicted here is linear.

a)

True

b)

False

15.

The graph depicted here is linear.

a)

True

b)

False

16.

This test detects the presence of which biological molecule?

a)

glucose

b)

lipids

c)

proteins

d)

starches

17.

The name of this test is?

a)

Benedicts

b)

Lugol's iodine

c)

Biurets

d)

Sudan IV

18.

This test is used to detect which biological molecule?

a)

lipids

b)

glucose

c)

starch

d)

proteins

19.

What is the name of the reagent depicted here?

a)

Benedicts

b)

Lugol's iodine

c)

Biurets

d)

Sudan IV

20.

What biological molecule is in the positive tube?

a)

protein

b)

glucose

c)

starch

d)

lipid

21.

What is the name of the reagent used in this test?

a)

Benedicts

b)

Lugol's iodine

c)

Biurets

d)

Sudan IV

22.

What is the name of the reagent used in this test?

a)

Benedicts

b)

Lugol's iodine

c)

Biurets

d)

Sudan IV

23.

What biological molecule is present in the left tube, but not in the right?

a)

protein

b)

starch

c)

glucose

d)

lipid

24.

What is the category of enzymes that break down the substance detected by the reagent in the right most test tube?

a)

amylase

b)

lipase

c)

protease

d)

b-galactosidase

25.

What is the category of enzymes that break down the substance detected by the reagent in the left test tube?

a)

amylase

b)

protease

c)

lipase

d)

b-galactosidase

26.

What is the category of enzymes that break down the substance detected by the reagent in the left test tube?

a)

lipase

b)

b-galactosidase

c)

protease

d)

amylase

27.

Glucose is broken down by amylase enzymes.

a)

True

b)

False

28.

Polymer proteins are broken down into?

a)

monosaccharides

b)

a monoglycerol and 2 fatty acid tails

c)

amino acids

d)

nucleotides

29.

How many sets of lenses magnify an object in this type of microscope?

a)

1

b)

2

c)

3

d)

4

30.

What is the total magnification of the specimen when the 40x objective is in place on this microscope?

a)

40x

b)

400x

c)

50x

d)

4000x

31.

If this is how the letter "e" appears when you look at your prepared slide, it is also how it appears through the oculars?

a)

Yes, only larger

b)

No, the orientation of the image is flipped

32.

Name the part of the microscope labeled A.

a)

fine focus knob

b)

course focus knob

c)

ocular

d)

objective

33.

Name the part of the microscope labeled B.

a)

Coarse focus knob

b)

Fine focus knob

c)

Stage controls

d)

Condensor

34.

Identify the labeled part of the scope that moves the slide forwards/backwards/left/right.

a)

A

b)

G

c)

E

d)

C

35.

Which letter represents the part of the microscope you would use to adjust the depth of field?

a)

A

b)

B

c)

C

d)

D

36.

What is the name the specialized structures stained purple in this cell?

a)

Anabaena

b)

Parmecium

c)

Elodea

d)

Amyloplasts

37.

Is this organism prokaryotic or eukaryotic?

a)

Prokaryotic

b)

Eukaryotic

38.

Name?

a)

Lactobacilus

b)

Anabaena

c)

Saccharomyces

d)

Elodea

39.

Prokaryotic or Eukaryotic?

a)

Prokaryotic

b)

Eukaryotic

40.

Name?

a)

Amyloplast

b)

Elodea

c)

Anabaena

d)

Paramecium

41.

Prokaryotic or Eukaryotic?

a)

Prokaryotic

b)

Eukaryotic

42.

Name?

a)

Paramecium

b)

Cheek Cells

c)

Saccharomyces

d)

Lactobacilus

43.

If you were to measure this cell to be 20 ocular units and had previously determined that each ocular unit is 5 micrometers in length, what is the actual length of the cell?

a)

100 micrometers

b)

25 micrometers

c)

4 micrometers

d)

100 millimeters

44.

Would this cell have a SA/V ratio greater or less than a paramecium cell?

a)

Greater than

b)

Less than

45.

What affect on EA do we see here?

a)

Nothing (Normal)

b)

Temperature

c)

Enzyme

46.

What was added to this solution to lower the EA of the reaction?

a)

Nothing (normal)

b)

Temperature

c)

Enzyme

47.

The arrow is pointing to what on the enzyme?

a)

Allosteric

b)

Active Site

c)

Activation Energy

d)

Substrate

48.

What is happening here?

a)

Competitive Inhibition

b)

Activation Energy

c)

Allosteric Bonding

d)

Coenzyme interactions

49.

A or B- which plant has the greater turgor pressure?

a)

A

b)

B

50.

Which of the following would you predict to have the fastest rate of diffusion?

a)

Ammonia diffusing in gas

b)

A cell with 20% sucrose in a 22% sucrose solution

c)

K-Permangante diffusion in agar

d)

Malachite green diffusing through liquid

51.

tonicity

a)

Hypotonic

b)

Isotonic

c)

Hypertonic

52.

Which way will water move?

a)

Into the cell

b)

Out of the cell

c)

No net movement

53.

Linear or Log?

a)

Linear

b)

Log

54.

What is this equation?

a)

Photosynthesis

b)

Cellular Respiration

55.

What was measured in the respiration lab to determine metabolic rate?

a)

CO2 production

b)

Heat production

c)

Oxygen Consumption

d)

Glucose Consumption

56.

What was the purpose of the KOH packets in the volumetric respirometers?

a)

Measure the amount of oxygen consumed

b)

Absorb CO2

c)

Absorb moisture in the test tube

d)

Feed the mealworms

57.

You are reviewing the following data: Cow 1,764 kcal/day, Chipmunk 254 kcal/day. What type of metabolic rate is this?

a)

Total MR

b)

msMR

58.

Enzymes end in...?

a)

-ose

b)

-ase

c)

-ate

59.

In salty water, salt would be the ____ and water would be the ____

a)

solute; solvent

b)

solvent; solute

c)

solute; solution

60.

What is the big "A" called?

a)

objective image

b)

real image

c)

virtual image

61.

What part of the microscope is the lens system that aligns and focuses the light from the lamp onto the specimen?

a)

Condensor

b)

Iris diaphragm

c)

Rheostat

d)

Lamp

62.

True or false: the condensor is placed in the light path to alter the amount of light that reaches the condenser (for enhancing contrast in the image)

a)

true

b)

false

63.

What factors affect rate of diffusion?

a)

Temperature

b)

Molecular mass

c)

Steepness of the concentration gradient

d)

Type of medium

64.

As temperature increases, rate of diffusion _______

a)

decreases

b)

stays the same

c)

increases

d)

depends on the solution

65.

What is the term used to describe when an enzyme loses its shape due to high heat or extreme pH?

a)

Apoptosis

b)

Denaturation

c)

Destruction

66.

B-galactosidase breaks ONPG down into:

a)

galactose and glucose

b)

lactose and o-nitrophenol

c)

galactose and o-nitrophenol

d)

lactose and glucose

67.

What were the two substrates we discussed in the enzymes lab?

a)

o-nitrophenol

b)

galactose

c)

lactose

d)

ONPG

68.

Are enzymes consumed by a reaction?

a)

yes

b)

no

69.

Enzymes help overcome activation energy of a reaction by adding energy to the system.

a)

True

b)

False