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WorksheetsMCQ Microbiology
Total questions: 120
Worksheet time: 2hrs 0mins
What is bacteria ?
What is a virus?
Viruses are single-celled microorganisms.
Viruses are multicellular organisms.
Viruses are not living organisms.
Viruses are plants.
What is the function of mitochondria?
Mitochondria are responsible for photosynthesis.
Mitochondria are the powerhouse of the cell.
Mitochondria help in cell division.
Mitochondria store water in the cell.
What is the function of ribosomes?
Ribosomes are responsible for photosynthesis.
Ribosomes are the powerhouse of the cell.
Ribosomes help in protein synthesis.
Ribosomes store water in the cell.
What is a fungus?
Fungi are single-celled microorganisms.
Fungi are multicellular organisms.
Fungi are not living organisms.
Fungi are plants.
What is the role of vacuoles in cells?
Vacuoles help in respiration.
Vacuoles store water and nutrients.
Vacuoles provide structural support to the plant.
Vacuoles help in seed dispersal.
What is the function of the nucleus in a cell?
The nucleus is responsible for photosynthesis.
The nucleus is the powerhouse of the cell.
The nucleus contains the genetic material of the cell.
The nucleus helps in protein synthesis.
What is the function of the cell membrane?
The cell membrane is responsible for photosynthesis.
The cell membrane is the powerhouse of the cell.
The cell membrane protect the cell.
The cell membrane helps in cell division.
What is the function of the endoplasmic reticulum?
The endoplasmic reticulum is responsible for photosynthesis.
The endoplasmic reticulum is the powerhouse of the cell.
The endoplasmic reticulum helps in protein synthesis and lipid metabolism.
The endoplasmic reticulum stores water in the cell.
What is the function of the Golgi apparatus?
The Golgi apparatus is responsible for photosynthesis.
The Golgi apparatus is the powerhouse of the cell.
The Golgi apparatus helps in protein modification and sorting.
The Golgi apparatus stores water in the cell.
What is the role of lysosomes in animal cells?
Lysosomes help in respiration.
Lysosomes store water and nutrients.
Lysosomes break down waste materials and cellular debris.
Lysosomes help in seed dispersal.
Father of Microbiology
Robert Hook
Louis Pasteur
Antonie van Leeuwenhoek
Robert Brown
Which of these is a cocci occurring in pairs?
Streptococci
Diplococci
Streptococci
Staphylococci
To differentiate between Gram positive and Gram negative bacteria _____________________ procedure will be used.
AFB Stain
Geimsa Stain
Gram Iodine
A Gram negative cell wall is __________ than a Gram positive one.
Thick
Hard
None of these
Which of the following statement is TRUE regarding Gram positive bacteria?
Cell wall have high lipid content
Lipopolysacharides layer is present
crystal violet stain is retained
Have 2 layers of cytoplasmic membrane.
What is the function of Pilus in prokaryotic?
for swimming movement
Attachment to surface
Transfer of DNA to other cells
Serve as defense mechanism
Pleomorphic is the term used particularly for bacteria having
One shape
Rod
Spiral
Variable shape
Bacteria are best measured in nanometers?
True
False
Prokaryotic and eukaryotic cells have many differences, but they also share some common features. Which of the following may be found in either type of cell?
Golgi bodies
mitochondria
ribosomes
nucleus
What is the purpose of pili?
To generate energy and transport chemicals
To allow the cell to stick to surfaces and transfer genetic material
To synthesize proteins
None of the things mentioned
What are the three basic shapes of bacteria?
Circular, triangular, and square
Spherical, rod-shaped, and spiral
Flat, curved, and elongated
Pointed, angular, and irregular
Bacteria are single-celled organisms that exist only inside other organisms.
true
false
All bacteria are harmful to humans.
true
false
Anaerobic bacteria can only grow in the presence of oxygen.
true
false
What is the difference between nutrient agar and lactose broth?
Nutrient agar is solid and lactose broth is liquid
Both are liquid but lactose broth has added lactose sugar
Nutrient agar is a selective media and lactose broth is a derived media
there is no difference between the two types
What is the bet pH for most bacterial growth?
2
4
7
11
If you want to select for a specific type of bacteria, what type of media would you use?
Nutrient broth
Differential media
Selective media
an autoclave
Nutrient broth is turned into nutrient agar by the addition of what?
lactose
eggs
agar
peptone
The type of culture media that can turn bacteria different colors on a petri dish is called a ____ media
selective
differential
natural
broth
This type of complex media is more rich than nutrient agar and includes the nutrient hemin.
chocolate agar
blood agar
LB agar
MacConkey agar
This type of complex media contains lysed red blood cells and additional nutrients.
blood agar
MacConkey agar
nutrient agar
chocolate agar
This type of media is composed of precise amounts of pure chemicals. It is essential when studying nutritional requirements of bacteria.
selective media
differential media
chemically defined media
enrichment culture media
This type of media inhibits the growth of organisms other than the one being grown. Can be complex or chemically defined media.
selective media
diferential media
chemically defined media
enrichment culture media
This type of media contains specific ingredients that help us to visually distinguish which species do or do not carry out a specific biochemical process.
selective media
differential media
chemically defined media
enrichment culture media
This type of media inhibits the growth of organisms other than the one being grown. Can be complex or chemically defined media.
selective media
diferential media
chemically defined media
enrichment culture media
This type of media enhances the growth of one particular species in a mixed population.
selective media
differential media
chemically defined media
enrichment culture media
This media selects for gram-negative organisms and isolates lactose fermenters (pink) and non-lactose fermenters (clear).
choclate agar
Mannitol salt agar
Eosin-methylene blue (EMB) agar
MacConkey agar
What is the difference between nutrient agar and lactose broth?
Nutrient agar is solid and lactose broth is liquid
Both are liquid but lactose broth has added lactose sugar
Nutrient agar is a selective media and lactose broth is a derived media
there is no difference between the two types
If you want to select for a specific type of bacteria, what type of media would you use?
Nutrient broth
Differential media
Selective media
an autoclave
Nutrient broth is turned into nutrient agar by the addition of what?
lactose
eggs
agar
peptone
Peptone provides this nutritional need for bacteria.
water
oxygen
protein
iron
All of these are derived media except
blood
nutrient agar
lactose broth
brilliant green bile broth
What is the difference between nutrient agar and nutrient broth.
one is liquid and one is solid
one is sterile and one is not sterile
one is acidic and one is basic
one has extra sugar and one does not
All of these options characterize natural media except
Natural media is difficult to sterilize
Natural media does have a long shelf life
Natural media is more challenging to collect from its source
Natural media comes in powder form and has to be put into solution
The liquid media is also termed as
Broth
Solution
Fluid
Slant
Which one of the following is not a source of nutrients for bacteria?
Peptone
Yeast extract
Agar
Beef extract
The concentration of agar in culture media is
1.5%
1%
.15%
2.55
Father of Microbiology is
Louis Pasteur
Lister
Robert Koch
Leeuwenhoek
E.coli was first isolated by
Hooke
Pasteur
Koch
Escherich
Hanging drop method for mobility study was given by
Koch
Pasteur
Hooke
Lister
The bacteria were first observed in the year 1675 by
Carolus Linnaeus
Alexander Fleming
Anton Von Leenuwenhoek
Robert Brown
The study of Fungi is
Algalogy
Mycology
Bacteriology
Phycology
Counter stain used in Gram's Staining is
Crystal Violet
Ethanol
Safranin
Malachite green
Gram positive bacteria
E.coli
Lactobacillus sp.
Pseudomonas sp.
All of the these
Gram staining is an example of
Acid fast staining
Simple staining
Negative staining
Differential staining
Which of the following is not a prokaryote?
Bacteria
Paramecium
Cyanobacteria
Rickettsia
A 40X objective and 10X produce a magnification of
50X
100X
400X
4000X
The first physician to make practical application of the germ theory of disease to surgery was
Joseph Lister
Louis Pastuer
Robert Koch
Edward Jenner
The primary use of Koch postulates is to
Clearly identify and characterize a particular microorganism
Isolate microbes from diseased animals
demonstrate that disease is caused by microorganisms
develop vaccines for specific disease
Mordant used in Gram's Staining is
Crystal Violet
Safranin
Ethanol
Iodine
Lipid contents are more in
Gram negative bacteria
Gram positive bacteria
Both
None of the above
The ability of microscope to distinguish between two objects into two separate objects is called
Resolving power
Numerical aperture
Wavelegth
Distance
Microorganisms are measured in ________
Micron
mm
Meter
cm
Disease causing microorganisms are called_______
Pathogens
Virus
Bacteria
Protozoa
The protein coat surrounding the virus is called ________
Envelope
Membrane
Capsid
Network
Antibiotic Penicillin obtained from___________.
Agaricus
Fusarium
Penicillium notatum
Pyricularia
Yeast reproduce by______"
Fission
Budding
Spore formation
Fragmentation
Malaria is caused by ______.
Influenza
Plasmodium
Vibrio cholera
Rhabdo Viridae
Which of the following is NOT a physical method of microbial control?
Radiation
Filtration
Heat
Antibiotics
Which of the following bacterial cell structures is involved in attachment to surfaces?
Capsule
Pili
Flagella
Endospore
Which of the following is NOT a way that bacteria can acquire resistance to antibiotics?
Mutation
Transformation
Conjugation
Fermentation
Bacteria are single-celled organisms that exist only inside other organisms.
true
false
The cell wall gives the bacteria its shape.
true
false
Which of the following is a part of cell envelope
Ribosome
Capsule
Gram positive and gram negative organisms are different from each other with respect to
Cell envelope
cytoplasm
Which of the following is bacterial appendage
pili
Hair
what is shape of streptococcus
spiral
spherical
Shape of helicobacter pylori
Bacilli
Spiral
Bacilli are
Rod shape
Round shape
What do you understand by aerobic respiration
Do not require oxygen for respiration
Require oxygen for respiration
Which of the following is true about obligate anerobes
killed by normal oygen in atmosphere
require oxygen for metabolism
What is the term used for organism that is infected
Host
Pathogen
What is true about opportunistic pathogen
causes in healthy host
cause disease in immunocompromised host
What do you understand by the binary fission
Increase in number of bacteria by multiplication
decraese in size of bacteria
Which phase refers to maturation of individual bacteria without division
log phase
lag phase
What is the term used for the phase in which bacteria die and do not divide
log phase
death phase
Optimum pH for bacterial growth is
6.5 to 7
6.9 to 9
Bacteria are mostly
Thermophiles
Mesophiles
Name the bacterial staining method in which on dye is used
compound staining
simple staining
To identify mycobacterium, which stain is used
Romanowsky staining
Ziehl nielsen stain
Crystal violet during gram staining of bacteria is retained in
gram positive
gram negative
Ziehl nielsen stain is an example of
gram staining
Acid fast staining
Management of bacterial infection includes
Antibiotics
Antiviral
_____is a prokaryotic microorganisms.
Virus
Algae
Fungi
Bacteria
______shows both living and non-living characteristics.
Protozoa
Virus
Bacteria
Fungi
Based on shape, the bacteria are of ______types.
2
3
4
5
Disease causing microorganisms are called_______
Pathogens
Virus
Bacteria
Protozoa
A village has an outbreak of cholera. Which would be the easiest way for a neighboring village to prevent the cholera epidemic from spreading to their village?
Develop a cholera vaccination.
Quarantine all individuals infected with cholera.
Avoid contaminated water, or treat contaminated water before drinking it.
Restrict travel to other villages that are farther away.
protein shells are also known as
Capsid
Nuclei
Cell Envelope
Chemical Machinery
Who invented the microscope
Louis Pasteur
Lister
Robert Koch
Leeuwenhoek
Which one of the following is not a type of microscope
Confocal Microscope
Floresence Microscope
Dark field Microscope
UV Light Microscope
corkscrew shaped bacteia are called
Cocci
Bacilli
vibrios
spirochaetes
which of the following can be used as a methd of disinfection
Heat
Gas or Vapour
Liquid
All of them
Identify B in the given image
Eye piece
Slide
Holder
Stage
Identify A in the given Image
Slide
Holder
Platform
Stage
Identify C in the given image
focal Lense
Lower lense
light source
adjustment lense
