WorksheetsPBS 1.1.6 DNA and Biotech
Total questions: 60
Worksheet time: 35mins
ATG TGA CAG?
Which of the following join together to form the rungs of DNA?
amino acids
nucleotides
fatty acids
polysaccharides
cytosine
guanine
thymine
uracil
What pictured here?.
RNA
Codon
nucleotide
The following choices is the correct pairing to DNA with ATGCCTGAT
UACGTACTU
UACGGACUA
TACGGACT
TACGGACTA
The type of sugar found in DNA is
Ribose sugar
Deoxy sugar
Deoxyribose
A segment of DNA contains 45% of Thymine. How much Adenine should be present?
55%
5%
45%
Where is Uracil found?
In DNA
in UNA
in RNA
30% Cytosine is found in a strand of DNA. How much Thymine must there be in the same strand?
20%
30%
40%
If a strand of DNA contains 15% Guanine, then how much Cytosine is there?
35%
15%
70%
Which of the following choices is the correct base pairing for the following code? TTG ACG GTA?
TTG ACG GTA
AAC ACG CAT
AAC TGC CAT
Guanine makes up 20% of a strand of DNA. Which of the following choices make up 30% of the strand?
Cytosine and Thymine
Thymine and Guanine
Both Thymine and Adenine
What does a gene code for?
DNA
a protein
A nitrogenous base
What 3 parts of DNA make up a nucleotide?
a base pair and a sugar
A base pair and phosphate
Phosphate, sugar and a single base
During the Translation process,
DNA codons are used to create mRNA
A duplicate copy of DNA is made
mRNA codons are used to create the amino acid chain.
White blood cells and stomach cells
Have the same DNA, and different genes are expressed (turned on)
Have the same DNA, and the same genes are expressed (turned on)
Have different parts of DNA, so they are always turned on.
In DNA, the A goes with which of the following?
U
C
G
T
In DNA, the C goes with which of the following?
T
A
G
U
In DNA, the Thymine goes with which of the following?
Uracil
Cytosine
Guanine
Adenine
In RNA, the C goes with which of the following?
Uracil
Guanine
Thymine
Adenine
What are we looking at?
DNA segments that are separated by size
Chromosomes
Recessive genes
Dominant genes
In order to produce these fragments, DNA must first be cut. What is used to cut DNA?
(a)
What are some applications for this technology? Select all that apply.
To compare DNA in forensic cases.
Missing person cases.
To show evolutionary relationships (how species are related)
To locate genes of interest based on size.
According to the gel electrophoresis results shown, which suspect committed the crime?
A
B
C
D
According to the gel electrophoresis results shown, which suspect committed the crime?
Suspect 1
Suspect 2
Suspect 3
not enough data to tell
The genetic material of all living things
genes
chromosomes
alleles
DNA
A method of amplifying (duplicating) small amounts of DNA evidence for use in investigation, testing, etc.
STRs
PCR
Intron
Exon
Which of the following DNA fragment sizes would be closer to the bottom of the gel?
250 bp
500 bp
750 bp
1000 bp
Which of the following best describes gel electrophoresis?
a technique used to make many copies of a specific region of DNA
a technique used to make many copies of the entire DNA sequence
a technique used to separate DNA fragments based on size and visualize them
a technique used to purify DNA
Approximately how many base pairs (bp) would be in the DNA fragment visualized on the example gel below?
200 bp
300 bp
500 bp
1000 bp
what is the another name for restriction enzymes
endonucleases
exonucleases
what are endonucleases
protein
carbohydrate
what does endonucleases do?
Cut DNA at specific sites
destroy DNA
Blunt ended DNA means
DNA with no single strands at end
DNA with single strands at ends
sticky ends means
DNA WITH single strand at ends with complementary bases
DNA with double strands
EcoRI is what?
Restriction enzyme from bacetria E. Coli
E. coli bacteria
the sequence of DNA where EcoRI acts is
GGAATTCC
GAATTC
According to this gel, who is most likely the child of the dad?
Child 1
Child 2
Both children are his.
Neither child is his.
True or False: You need to use a new tip with each new sample of DNA.
True
False
The area on the gel where you load the DNA is called the ____.
wells
positive pole
digestion site
loading site
The larger fragments of DNA will ...
remain closer to the wells.
travel towards the + pole of the gel.
not move at all.
travel farther than the smaller fragments.
DNA has a _____ charge.
positive
negative
neutral
unknown
The process that separates the cut fragments of DNA by their size and compares them is called ____.
PCR
digestion
gel electrophoresis
restriction enzymes
If a restriction enzyme makes a cut that is straight up and down through the double strand of DNA, this is called creating ______ ends.
sticky
blunt
straight
organized
If a restriction enzyme cuts the DNA 3 times, how many fragments (RFLPs) were made?
1
2
3
4
If a restriction enzyme cuts the DNA 3 times, how many recognition sites are there on this DNA?
1
2
3
4
The job of restriction enzymes is to ...
copy the DNA.
cut the DNA at specific locations.
remove the DNA.
organize the DNA.
Restriction enzymes come from ...
viruses
bacteria
plants
animals
The process of ______ is used to create more copies of a specific sequence of DNA.
PCR
Gel Electrophoresis
Micropipetting
Digestion
What base is the orange nucleotide?
adenine
cytosine
guanine
thymine
Which two bases are pyrimidines?
adenine and thymine
adenine and guanine
cytosine and guanine
cytosine and thymine
