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WorksheetsTLC and HPLC
Total questions: 24
Worksheet time: 16mins
What is HPLC?
A technique to separate components based on pH
A technique to separate components based on polarity
A technique to separate components based on liquidity
A technique to separate components based on temperatue
What is the function of degasser?
To add gas into the system of HPLC
To prevent gas into the system of HPLC
To change liquid into gas
To change gas into liquid
Which substance is used in normal phased HPLC?
stationary phase: high polar
mobile phase: low polar
Stationary phase: high polar
Mobile phase: high polar
Stationary phase: low polar
Mobile phase: low polar
Stationary phase: low polar
Mobile phase: high polar
Solid phase extraction is used to ...
purify the samples from unwanted components
purify the solvents from unwanted components
purify the samples using low elution strength
purify the samples using high elution strength
Water / methanol is used as a mobile phase in ...
Normal phase of HPLC
Reverse phase of HPLC
What happens to the given substance under reverse phase conditions when the pH of the mobile phase is raised?
Retention time doesn't change
Retention time will increase
Retention time will decrease
What is the most commonly used stationary phase in reversed phase chromatography?
C18
Silicagel
C8
Carbon
HPLC methods include
liquid/liquid (partition) chromatography
liquid/solid (adsorption) chromatography
ion exchange nd size exclusion chromatography
all of the option correct
Based on a normal chromatography, the stationary phase is polar.
TRUE
FALSE
Depends on the substance
Which one of these do not associate with the HPLC component?
UV detector
Pump
Degasser
Atomizer
Choose the wrong statement regarding the column.
Usually made of stainless steel material
Packing materials commonly used are silica or resin
Longer column will give longer time for the mixture to separate and thus gives better resolution
Higher column ID can provide higher separation sensitivity
What is the mechanism for HPLC?
I. The mobile phase
II. Auto sampler
III. Atomizer
IV. UV detector
I, II
III, IV
III
IV
What is HPLC consist of what?
Detector
CDS (Chromatography data system)
column
Pumping system
Cuvette of Colorimeter is made of-
Alkali halide
Quartz
Glass
All of these
How to calculate Rf?
Rf = distance travelled by sample / distance travelled by solvent
Rf = distance travelled by solvent / distance travelled by sample
The retention factor is used
to zero the chromatography scale
to identify unknown compounds in a sample
to retain solvent
as a starting tool for chromatography
Thin layer chromatography is
partition chromatography
electrical mobility of ionic species
adsorption chromatography
none of the above
The presence of more than one spot on the TLC plate shows that____
the Rf value is higher than it should be
the solvent used was too dilute
impurities are present
too much solvent was used
Which of the following is correct about chromatography?
It separates those solutes that dissolve in the same solvent.
It separates those solutes that dissolve in different solvents.
It separates a solute that dissolves in a solid solvent.
It separates gaseous solutes from liquid solvents.
chromatography separates the mixture of dyes on the basis of their ---------------------------
gravity
solubility
density
boiling point
The advantage of centrifugal TLC over preparative TLC is .......
Simpler collection of target compound
Adjustable thickness of silica
No need for a pumping system
No need for a rotor
Which of the following system has the thickest layer of stationary phase?
Ultra TLC
HP TLC
Preparative TLC
TLC-MS
